gld-3 Defective in germ line development protein 3 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: gld-3
Official Full Name: Defective in germ line development protein 3
Primary source: WormBase:WBGene00001597
Locus tag: CELE_T07F8.3
See related: AllianceGenome:WB:WBGene00001597
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables polynucleotide adenylyltransferase activator activity and protein domain specific binding activity. Involved in several processes, including cytosolic mRNA polyadenylation; masculinization of hermaphroditic germ-line; and positive regulation of meiotic nuclear division. Located in P granule. Part of RNA-directed RNA polymerase complex. Is expressed in germ line and in male. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See gld-3 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 20

Sequence:: Chromosome: II; NC_003280.10 (7132643..7137522)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

picture that emerges from the structural and biochemical data are that GLD-3 activates GLD-2 both indirectly by stabilizing the enzyme and directly by contributing positively charged residues near the RNA-binding cleft
Title: Structural basis for the activation of the C. elegans noncanonical cytoplasmic poly(A)-polymerase GLD-2 by GLD-3.
Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding.
Title: A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex.
report the 2.8-A-resolution crystal structure of a proteolytically stable fragment encompassing the KH2, KH3, KH4, and KH5 domains of C. elegans GLD-3
Title: Four KH domains of the C. elegans Bicaudal-C ortholog GLD-3 form a globular structural platform.
In C. elegans hermaphrodites, gamete production begins with spermatogenesis and transitions later to oogenesis; the combinatorial use of GLD-2 contributes to this transition, as GLD-2/GLD-3 promotes spermatogenesis, and GLD-2/RNP-8 specifies oogenesis.
Title: Antagonism between GLD-2 binding partners controls gamete sex.
In germ cells, GLD-3 promotes the transition from mitosis to meiosis primarily via its interaction with GLD-2, and it promotes spermatogenesis primarily via its interaction with FBF.
Title: GLD-3 and control of the mitosis/meiosis decision in the germline of Caenorhabditis elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T07F8.4 (e-PCR)
- UniSTS:306270

T07F8.3 (e-PCR)
- UniSTS:310726

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables polynucleotide adenylyltransferase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cytosolic mRNA polyadenylation	IDA Inferred from Direct Assay more info	PubMed
involved_in embryo development ending in birth or egg hatching	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-line sex determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-line stem cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in masculinization of hermaphroditic germ-line	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nematode larval development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of meiotic nuclear division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vulval development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
part_of RNA-directed RNA polymerase complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info