Klf3 Kruppel-like transcription factor 3 (basic) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Klf3provided by MGI
Official Full Name: Kruppel-like transcription factor 3 (basic)provided by MGI
Primary source: MGI:MGI:1342773
See related: Ensembl:ENSMUSG00000029178 AllianceGenome:MGI:1342773
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Bklf; Tef-2; 9930027G08Rik
Summary: Enables RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleoplasm. Is expressed in several structures, including alimentary system; brain; early conceptus; genitourinary system; and integumental system. Orthologous to human KLF3 (KLF transcription factor 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in colon adult (RPKM 33.8), thymus adult (RPKM 20.9) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 C3.1; 5 33.49 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (64960864..64990246)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (64803521..64832901)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

KLF3 Transcription Activates WNT1 and Promotes the Growth and Metastasis of Gastric Cancer via Activation of the WNT/beta-Catenin Signaling Pathway.
Title: KLF3 Transcription Activates WNT1 and Promotes the Growth and Metastasis of Gastric Cancer via Activation of the WNT/β-Catenin Signaling Pathway.
miR-324-5p promotes adipocyte differentiation and lipid droplet accumulation by targeting Krueppel-like factor 3 (KLF3).
Title: miR-324-5p promotes adipocyte differentiation and lipid droplet accumulation by targeting Krueppel-like factor 3 (KLF3).
Kruppel-like factor 3 (KLF3) suppresses NF-kappaB-driven inflammation in mice.
Title: Krüppel-like factor 3 (KLF3) suppresses NF-κB-driven inflammation in mice.
KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.
Title: KLF3 promotes the 8-cell-like transcriptional state in pluripotent stem cells.
miR-20a-5p promotes adipocyte differentiation from bone marrow-derived stromal cells by targeting and negatively regulating Klf3 in the early phase during the procedure of adipogenesis.
Title: miR-20a-5p promotes adipogenic differentiation of murine bone marrow stromal cells via targeting Kruppel-like factor 3.
KLF1 and KLF3 bind common as well as unique sites within the erythroid cell genome.
Title: Krüppel-like factors compete for promoters and enhancers to fine-tune transcription.
mechanistic insights into the regulation of Lgals3, demonstrating that C-terminal binding protein (CtBP) is required to drive optimal KLF3-mediated silencing.
Title: Krüppel-like Factor 3 (KLF3/BKLF) Is Required for Widespread Repression of the Inflammatory Modulator Galectin-3 (Lgals3).
Cineromycin B inhibited adipocyte differentiation in 3T3-L1 cells mainly via upregulation of KLF2 and KLF3 mRNA expression at an early stage of the differentiation.
Title: Cineromycin B isolated from Streptomyces cinerochromogenes inhibits adipocyte differentiation of 3T3-L1 cells via Krüppel-like factors 2 and 3.
Phosphorylation of KLF3 and CtBP2 by HIPK2 strengthens the interaction between these two factors and increases transcriptional repression by KLF3.
Title: Phosphorylation of Krüppel-like factor 3 (KLF3/BKLF) and C-terminal binding protein 2 (CtBP2) by homeodomain-interacting protein kinase 2 (HIPK2) modulates KLF3 DNA binding and activity.
KLF3 acts as transcriptional repressor for the leukocyte-specific integrin beta7 (Itgb7, Ly69) by binding to the beta7 promoter, as revealed by chromatin immunoprecipitation.
Title: Leukocyte β7 integrin targeted by Krüppel-like factors.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (2)
Gene trapped (2)
Targeted (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH141772 (e-PCR)
- UniSTS:223765

Klf3 (e-PCR), detects polymorphism
- UniSTS:143697

Klf3 (e-PCR), detects polymorphism
- UniSTS:502664

NoName (e-PCR)
- UniSTS:492506

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	IC Inferred by Curator more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific double-stranded DNA binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to peptide	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: Krueppel-like factor 3

Names: CACCC-box-binding protein BKLF; Kruppel-like factor 3 (basic); basic krueppel-like factor

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008453.6 → NP_032479.1 Krueppel-like factor 3

See identical proteins and their annotated locations for NP_032479.1

Status: VALIDATED

Source sequence(s)

AC130535

Consensus CDS

CCDS19301.1

UniProtKB/Swiss-Prot

Q60980

UniProtKB/TrEMBL

Q545J5

Related

ENSMUSP00000129363.2, ENSMUST00000165536.8

Conserved Domains (4) summary

sd00017
Location:261 → 283

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:259 → 283

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:305 → 330

zf-H2C2_2; Zinc-finger double domain

cd21577
Location:49 → 259

KLF3_N; N-terminal domain of Kruppel-like factor 3

RNA

NR_165035.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC130535

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

64960864..64990246

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006503751.5 → XP_006503814.1 Krueppel-like factor 3 isoform X1

See identical proteins and their annotated locations for XP_006503814.1

UniProtKB/Swiss-Prot

Q60980

UniProtKB/TrEMBL

Q545J5

Conserved Domains (4) summary

sd00017
Location:261 → 283

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam00096
Location:259 → 283

zf-C2H2; Zinc finger, C2H2 type

pfam13465
Location:305 → 330

zf-H2C2_2; Zinc-finger double domain

cd21577
Location:49 → 259

KLF3_N; N-terminal domain of Kruppel-like factor 3