Htr4 5 hydroxytryptamine (serotonin) receptor 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Htr4provided by MGI
Official Full Name: 5 hydroxytryptamine (serotonin) receptor 4provided by MGI
Primary source: MGI:MGI:109246
See related: Ensembl:ENSMUSG00000026322 AllianceGenome:MGI:109246
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: 5-HT4; 5HTR4; 5-HT-4; 5-HT<4L>
Summary: Enables G protein-coupled serotonin receptor activity. Involved in several processes, including maintenance of gastrointestinal epithelium; mucus secretion; and regulation of postsynapse assembly. Is active in glutamatergic synapse; plasma membrane; and postsynapse. Is expressed in several structures, including brain; gallbladder; genitourinary system; gut; and spinal cord. Used to study anorexia nervosa. Orthologous to human HTR4 (5-hydroxytryptamine receptor 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in bladder adult (RPKM 1.5), colon adult (RPKM 1.5) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18 E1; 18 35.1 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (62456618..62636098)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (62323546..62503027)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Activation of the 5-hydroxytryptamine 4 receptor ameliorates tight junction barrier dysfunction in the colon of type 1 diabetic mice.
Title: Activation of the 5-hydroxytryptamine 4 receptor ameliorates tight junction barrier dysfunction in the colon of type 1 diabetic mice.
Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine.
Title: Expression of serotonin receptor HTR4 in glucagon-like peptide-1-positive enteroendocrine cells of the murine intestine.
Serotonin 5-HT4 receptor boosts functional maturation of dendritic spines via RhoA-dependent control of F-actin.
Title: Serotonin 5-HT(4) receptor boosts functional maturation of dendritic spines via RhoA-dependent control of F-actin.
Findings of the present in vitro study enrich the understanding and provide insight roles of 5-HT4R in embryonic brain development by promoting the growth of hippocampal neurons.
Title: Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice.
Synaptic potentiation is mediated by 5-HT4 receptors and that systemic modulation of 5-HT4 receptor function can bidirectionally impact memory formation.
Title: Hippocampal 5-HT Input Regulates Memory Formation and Schaffer Collateral Excitation.
Impeding serotonergic signaling preferentially gates spike-timing-dependent long-term depression (t-LTD) at thalamostriatal synapses. This t-LTD requires dampened activity of the 5-HT4 receptor subtype.
Title: Serotonergic Signaling Controls Input-Specific Synaptic Plasticity at Striatal Circuits.
Gut microbiota-produced Tryptamine activates epithelial 5-HT4R serotonin receptor to increase cAMP level and drive colonic fluid secretion.
Title: Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion.
Prefrontal cortex 5-HT4Rs mediate hypophagia following stress.
Title: Adaptive Control of Dorsal Raphe by 5-HT4 in the Prefrontal Cortex Prevents Persistent Hypophagia following Stress.
Knock down serotonin receptors 5-HTR3 and 5-HTR4 in neonatal cardiomyocytes resulted in significant increase of cell damage in response to hypoxia, and also led to alternation in heart beating.
Title: 5-HTR3 and 5-HTR4 located on the mitochondrial membrane and functionally regulated mitochondrial functions.
Study shows that in the murine gastrointestinal tract, activation of 5-HT4 receptors with prucalopride enhances cholinergic contractions, illustrating facilitation of myenteric cholinergic neurotransmission.
Title: 5-HT(4) receptors facilitate cholinergic neurotransmission throughout the murine gastrointestinal tract.

Submit: New GeneRIF Correction See all GeneRIFs (45)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (4) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:494136

D18Mit228 (e-PCR), detects polymorphism
- UniSTS:116804

Htr4 (e-PCR), detects polymorphism
- UniSTS:495967

NoName (e-PCR)
- UniSTS:489070

38.MMHAP81FRE11.seq (e-PCR)
- UniSTS:124298

D18Mit139 (e-PCR), detects polymorphism
- UniSTS:128161

Htr4 (e-PCR), detects polymorphism
- UniSTS:532457

Htr4 (e-PCR), detects polymorphism
- UniSTS:532458

Htr4 (e-PCR), detects polymorphism
- UniSTS:532459

Htr4 (e-PCR), detects polymorphism
- UniSTS:532460

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein-coupled serotonin receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled serotonin receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables G protein-coupled serotonin receptor activity	ISO Inferred from Sequence Orthology more info
enables G protein-coupled serotonin receptor activity	ISS Inferred from Sequence or Structural Similarity more info
enables neurotransmitter receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables serotonin binding	IBA Inferred from Biological aspect of Ancestor more info
enables serotonin receptor activity	IEA Inferred from Electronic Annotation more info
enables serotonin receptor activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	IBA Inferred from Biological aspect of Ancestor more info
involved_in G protein-coupled serotonin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in adenylate cyclase-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in adenylate cyclase-activating serotonin receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in adenylate cyclase-inhibiting serotonin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in gamma-aminobutyric acid signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in large intestinal transit	IEA Inferred from Electronic Annotation more info
involved_in large intestinal transit	ISO Inferred from Sequence Orthology more info
involved_in maintenance of gastrointestinal epithelium	IDA Inferred from Direct Assay more info	PubMed
involved_in mucus secretion	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of appetite	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynapse assembly	EXP Inferred from Experiment more info	PubMed
involved_in regulation of postsynapse assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 5-hydroxytryptamine receptor 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001364956.1 → NP_001351885.1 5-hydroxytryptamine receptor 4 isoform 2

Status: VALIDATED

Source sequence(s)

AC131714, AC152454

Consensus CDS

CCDS89255.1

UniProtKB/TrEMBL

A0A494BA82

Related

ENSMUSP00000157982.2, ENSMUST00000237114.2

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_001364957.1 → NP_001351886.1 5-hydroxytryptamine receptor 4 isoform 3

Status: VALIDATED

Source sequence(s)

AC131714, AC152454

Consensus CDS

CCDS89257.1

Related

ENSMUSP00000157967.2, ENSMUST00000238081.2

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_001364958.1 → NP_001351887.1 5-hydroxytryptamine receptor 4 isoform 4

Status: VALIDATED

Source sequence(s)

AC131714, AC152454

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_001364959.1 → NP_001351888.1 5-hydroxytryptamine receptor 4 isoform 5

Status: VALIDATED

Source sequence(s)

AC131714, AC152454

Consensus CDS

CCDS89256.1

Related

ENSMUSP00000157470.2, ENSMUST00000236691.2

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
NM_008313.4 → NP_032339.2 5-hydroxytryptamine receptor 4 isoform 1

See identical proteins and their annotated locations for NP_032339.2

Status: VALIDATED

Source sequence(s)

AC131714, AC152454

Consensus CDS

CCDS29291.1

UniProtKB/Swiss-Prot

O89003, O89004, P97288, Q3URB5, Q9R2A4

Related

ENSMUSP00000027560.7, ENSMUST00000027560.8

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

62456618..62636098

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030250347.2 → XP_030106207.1 5-hydroxytryptamine receptor 4 isoform X6

Conserved Domains (2) summary

cd14964
Location:21 → 47

7tm_GPCRs; TM helix 1 [structural motif]

cl28897
Location:20 → 118

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily
XM_006525682.5 → XP_006525745.1 5-hydroxytryptamine receptor 4 isoform X3

See identical proteins and their annotated locations for XP_006525745.1

UniProtKB/TrEMBL

A0A494BA82

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_006525681.5 → XP_006525744.1 5-hydroxytryptamine receptor 4 isoform X3

See identical proteins and their annotated locations for XP_006525744.1

UniProtKB/TrEMBL

A0A494BA82

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_006525683.5 → XP_006525746.1 5-hydroxytryptamine receptor 4 isoform X4

See identical proteins and their annotated locations for XP_006525746.1

UniProtKB/Swiss-Prot

P97288

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_030250345.2 → XP_030106205.1 5-hydroxytryptamine receptor 4 isoform X2

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_011246848.4 → XP_011245150.1 5-hydroxytryptamine receptor 4 isoform X5

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_006525680.5 → XP_006525743.1 5-hydroxytryptamine receptor 4 isoform X2

See identical proteins and their annotated locations for XP_006525743.1

UniProtKB/Swiss-Prot

P97288

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_030250344.2 → XP_030106204.1 5-hydroxytryptamine receptor 4 isoform X1

UniProtKB/Swiss-Prot

O89003, O89004, P97288, Q3URB5, Q9R2A4

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_030250343.2 → XP_030106203.1 5-hydroxytryptamine receptor 4 isoform X1

UniProtKB/Swiss-Prot

O89003, O89004, P97288, Q3URB5, Q9R2A4

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_006525676.5 → XP_006525739.1 5-hydroxytryptamine receptor 4 isoform X1

See identical proteins and their annotated locations for XP_006525739.1

UniProtKB/Swiss-Prot

O89003, O89004, P97288, Q3URB5, Q9R2A4

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors
XM_006525678.5 → XP_006525741.1 5-hydroxytryptamine receptor 4 isoform X1

See identical proteins and their annotated locations for XP_006525741.1

UniProtKB/Swiss-Prot

O89003, O89004, P97288, Q3URB5, Q9R2A4

Conserved Domains (1) summary

cd15056
Location:20 → 323

7tmA_5-HT4; serotonin receptor subtype 4, member of the class A family of seven-transmembrane G protein-coupled receptors