Dgkh diacylglycerol kinase, eta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Dgkhprovided by MGI
Official Full Name: diacylglycerol kinase, etaprovided by MGI
Primary source: MGI:MGI:2444188
See related: Ensembl:ENSMUSG00000034731 AllianceGenome:MGI:2444188
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DGK; D130015C16; 5930402B05Rik
Summary: Enables ATP-dependent diacylglycerol kinase activity. Involved in diacylglycerol metabolic process; lipid phosphorylation; and phosphatidic acid biosynthetic process. Located in actin cytoskeleton; cytoplasm; and plasma membrane. Is expressed in nervous system and neural retina. Orthologous to human DGKH (diacylglycerol kinase eta). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in testis adult (RPKM 3.4), cortex adult (RPKM 2.7) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 D3; 14 41.56 cM

Exon count:: 40

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (78796789..78970169, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (78559349..78732729, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Diacylglycerol kinase eta regulates cell proliferation and its levels are elevated by glucocorticoids in undifferentiated neuroblastoma cells.
Title: Diacylglycerol kinase η regulates cell proliferation and its levels are elevated by glucocorticoids in undifferentiated neuroblastoma cells.
Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase eta-knockout mouse brain.
Title: Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase η-knockout mouse brain.
Diacylglycerol kinase eta regulates C2C12 myoblast proliferation through the mTOR signaling pathway.
Title: Diacylglycerol kinase η regulates C2C12 myoblast proliferation through the mTOR signaling pathway.
Mania- and anxiety-like behavior and impaired maternal care in female diacylglycerol kinase eta and iota double knockout mice.
Title: Mania- and anxiety-like behavior and impaired maternal care in female diacylglycerol kinase eta and iota double knockout mice.
This studies revealed the dgkh expressional changes during mouse brain development.
Title: Expressional profile of the diacylglycerol kinase eta gene DGKH.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (5) 1 citation
Gene trapped (1)
Targeted (1) 1 citation

General gene information

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Markers

RH143051 (e-PCR)
- UniSTS:232298

Dgkh (e-PCR), detects polymorphism
- UniSTS:546734

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables SAM domain binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of catalytic activity	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: diacylglycerol kinase eta

Names: DAG kinase eta; DGK-eta; diglyceride kinase eta

NP_001074805.2

EC 2.7.1.107

NP_001240695.1

EC 2.7.1.107

NP_001268723.1

EC 2.7.1.107

XP_011243402.1

EC 2.7.1.107

XP_030103727.1

EC 2.7.1.107

XP_036014587.1

EC 2.7.1.107

XP_036014588.1

EC 2.7.1.107

XP_036014589.1

EC 2.7.1.107

XP_036014590.1

EC 2.7.1.107

XP_036014591.1

EC 2.7.1.107

XP_036014592.1

EC 2.7.1.107

XP_036014593.1

EC 2.7.1.107

XP_036014594.1

EC 2.7.1.107

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081336.2 → NP_001074805.2 diacylglycerol kinase eta isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

Source sequence(s)

AC124715, AC126251, AC161608

Consensus CDS

CCDS36986.2

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

UniProtKB/TrEMBL

A0A2I3BQ48

Related

ENSMUSP00000154036.3, ENSMUST00000226342.3

Conserved Domains (5) summary

smart00109
Location:269 → 313

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:786 → 943

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:355 → 477

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:197 → 246

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd13274
Location:88 → 184

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain
NM_001253766.2 → NP_001240695.1 diacylglycerol kinase eta isoform 2

See identical proteins and their annotated locations for NP_001240695.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and has multiple differences in the coding region, one of which results in a translational frameshift, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus, a distinct C-terminus, and is shorter than isoform 1.

Source sequence(s)

AC161608, AK083819, AV276089, CK780920

Consensus CDS

CCDS88719.1

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

UniProtKB/TrEMBL

A0JP53

Related

ENSMUSP00000154554.2, ENSMUST00000228362.2

Conserved Domains (5) summary

cd09576
Location:1006 → 1070

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:629 → 786

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:198 → 320

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20848
Location:11 → 96

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:107 → 168

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins
NM_001281794.2 → NP_001268723.1 diacylglycerol kinase eta isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) differs in both UTRs and has multiple differences in the coding region, one of which results in a translational frameshift, compared to variant 1. The resulting protein (isoform 3) has a shorter N-terminus, a distinct C-terminus, and is shorter than isoform 1.

Source sequence(s)

AC126251, AC161608, AK083819

Consensus CDS

CCDS88720.1

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

UniProtKB/TrEMBL

A0A2I3BQ43

Related

ENSMUSP00000154031.2, ENSMUST00000227767.2

Conserved Domains (5) summary

smart00109
Location:112 → 156

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:629 → 786

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:198 → 320

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:40 → 89

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cl17171
Location:1 → 27

PH-like; Pleckstrin homology-like domain

RNA

NR_104044.2 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses an alternate 5' exon structure and lacks an alternate internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most supported translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AC126251, AC161608, AK133314

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

78796789..78970169 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011245100.3 → XP_011243402.1 diacylglycerol kinase eta isoform X2

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (6) summary

smart00109
Location:269 → 313

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:786 → 943

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:355 → 477

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:197 → 246

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd13274
Location:88 → 184

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cl15755
Location:1163 → 1227

SAM_superfamily; SAM (Sterile alpha motif )
XM_036158694.1 → XP_036014587.1 diacylglycerol kinase eta isoform X1

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (6) summary

cd09576
Location:1174 → 1238

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:786 → 954

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:355 → 477

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd13274
Location:88 → 184

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cd20848
Location:168 → 253

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:264 → 325

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins
XM_036158695.1 → XP_036014588.1 diacylglycerol kinase eta isoform X4

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (6) summary

cd09576
Location:1150 → 1214

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:762 → 930

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:331 → 453

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd13274
Location:64 → 160

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain

cd20848
Location:144 → 229

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:240 → 301

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins
XM_036158700.1 → XP_036014593.1 diacylglycerol kinase eta isoform X9

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

UniProtKB/TrEMBL

A0JP53

Conserved Domains (5) summary

cd09576
Location:1006 → 1070

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:629 → 786

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:198 → 320

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20848
Location:11 → 96

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:107 → 168

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins
XM_036158699.1 → XP_036014592.1 diacylglycerol kinase eta isoform X8

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (5) summary

cd09576
Location:1017 → 1081

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:629 → 797

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:198 → 320

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20848
Location:11 → 96

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:107 → 168

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins
XM_036158698.1 → XP_036014591.1 diacylglycerol kinase eta isoform X7

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (6) summary

cd09576
Location:1029 → 1093

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:641 → 809

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:210 → 332

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20848
Location:23 → 108

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:119 → 180

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cl17171
Location:3 → 39

PH-like; Pleckstrin homology-like domain
XM_036158696.1 → XP_036014589.1 diacylglycerol kinase eta isoform X5

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (6) summary

cd09576
Location:1047 → 1111

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:659 → 827

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:228 → 350

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20848
Location:41 → 126

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:137 → 198

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cl17171
Location:22 → 57

PH-like; Pleckstrin homology-like domain
XM_036158697.1 → XP_036014590.1 diacylglycerol kinase eta isoform X6

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

Conserved Domains (6) summary

cd09576
Location:1037 → 1101

SAM_DGK-eta; SAM domain of diacylglycerol kinase eta

smart00045
Location:649 → 817

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:218 → 340

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20848
Location:31 → 116

C1_DGKeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cd20894
Location:127 → 188

C1_DGKeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase eta (DAG kinase eta) and similar proteins

cl17171
Location:11 → 47

PH-like; Pleckstrin homology-like domain
XM_036158701.1 → XP_036014594.1 diacylglycerol kinase eta isoform X10

Conserved Domains (2) summary

smart00045
Location:313 → 481

DAGKa; Diacylglycerol kinase accessory domain (presumed)

cl15755
Location:701 → 765

SAM_superfamily; SAM (Sterile alpha motif )
XM_030247867.2 → XP_030103727.1 diacylglycerol kinase eta isoform X3

UniProtKB/Swiss-Prot

A0A0B5JC35, D3YXJ0

UniProtKB/TrEMBL

A0A2I3BQ48

Conserved Domains (5) summary

smart00109
Location:269 → 313

C1; Protein kinase C conserved region 1 (C1) domains (Cysteine-rich domains)

smart00045
Location:786 → 943

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:355 → 477

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd00029
Location:197 → 246

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd13274
Location:88 → 184

PH_DGK_type2; Type 2 Diacylglycerol kinase Pleckstrin homology (PH) domain