PKN3 protein kinase N3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: PKN3provided by HGNC
Official Full Name: protein kinase N3provided by HGNC
Primary source: HGNC:HGNC:17999
See related: Ensembl:ENSG00000160447 MIM:610714; AllianceGenome:HGNC:17999
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: UTDP4-1
Summary: Predicted to enable protein serine/threonine kinase activity. Involved in epithelial cell migration. Predicted to be located in Golgi apparatus; cytosol; and nucleus. [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in fat (RPKM 3.9), lung (RPKM 3.1) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 9q34.11

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	9	NC_000009.12 (128702503..128720916)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	9	NC_060933.1 (140907265..140925683)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	9	NC_000009.11 (131464782..131483195)

Chromosome 9 - NC_000009.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18.
Title: A Screen for PKN3 Substrates Reveals an Activating Phosphorylation of ARHGAP18.
a novel interaction of p130Cas with Ser/Thr kinase PKN3, is reported.
Title: The interaction of p130Cas with PKN3 promotes malignant growth.
Steady-state kinetic analysis revealed that PKN1-3 follows a sequential ordered Bi-Bi kinetic mechanism, where peptide substrate binding is preceded by ATP binding. This kinetic mechanism was confirmed by additional kinetic studies for product inhibition and affinity of small molecule inhibitors.
Title: Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.
PKN3 is the major regulator of angiogenesis in humans and mice and tumor metastasis in mice.
Title: PKN3 is the major regulator of angiogenesis and tumor metastasis in mice.
PKN3 can be considered a novel protein implicated in remodeling the actin-adherens junction, possibly by linking ICAM-1-signaling with actin/AJ dynamics.
Title: Depletion of protein kinase N3 (PKN3) impairs actin and adherens junctions dynamics and attenuates endothelial cell activation.
PKN isoforms are not simply redundant in supporting migration, but appear to be linked through isoform specific regulatory domain properties to selective upstream signals. It
Title: Regulatory domain selectivity in the cell-type specific PKN-dependence of cell migration.
PKN3 might represent a preferred target for therapeutic intervention in cancers that lack tumor suppressor PTEN function or depend on chronic activation of phosphoinositide 3-kinase
Title: PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase.

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Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genetic variants associated with disordered eating. EBI GWAS Catalog EBI GWAS CatalogPubMed
Nine loci for ocular axial length identified through genome-wide association studies, including shared loci with refractive error. EBI GWAS Catalog EBI GWAS CatalogPubMed
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PKN3_9611 (e-PCR)
- UniSTS:468602

STS-N29855 (e-PCR)
- UniSTS:7411

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	TAS Traceable Author Statement more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in epithelial cell migration	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	TAS Traceable Author Statement more info	PubMed
involved_in signal transduction	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	TAS Traceable Author Statement more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase N3

Names: protein kinase PKN-beta; protein-kinase C-related kinase 3

NP_001304855.1

EC 2.7.11.13

NP_037487.2

EC 2.7.11.13

XP_005252003.1

EC 2.7.11.13

XP_006717143.1

EC 2.7.11.13

XP_016870138.1

EC 2.7.11.13

XP_016870139.1

EC 2.7.11.13

XP_047279234.1

EC 2.7.11.13

XP_047279235.1

EC 2.7.11.13

XP_054218798.1

EC 2.7.11.13

XP_054218799.1

EC 2.7.11.13

XP_054218800.1

EC 2.7.11.13

XP_054218801.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001317926.2 → NP_001304855.1 serine/threonine-protein kinase N3 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the penultimate exon, compared to variant 1, resulting in a novel 3' coding region and shorter 3' UTR. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AL441992

Conserved Domains (6) summary

cd11622
Location:13 → 78

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11632
Location:97 → 170

HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3

smart00220
Location:559 → 811

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl00087
Location:170 → 230

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:328 → 410

C2; C2 domain

cl21453
Location:559 → 815

PKc_like; Protein Kinases, catalytic domain
NM_013355.5 → NP_037487.2 serine/threonine-protein kinase N3 isoform 1

See identical proteins and their annotated locations for NP_037487.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AL441992

Consensus CDS

CCDS6908.1

UniProtKB/Swiss-Prot

Q6P5Z2, Q9UM03

Related

ENSP00000291906.4, ENST00000291906.5

Conserved Domains (6) summary

PTZ00263
Location:555 → 879

PTZ00263; protein kinase A catalytic subunit; Provisional

cd11622
Location:13 → 78

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11632
Location:97 → 170

HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3

cd05589
Location:559 → 884

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:170 → 230

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:328 → 410

C2; C2 domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000009.12 Reference GRCh38.p14 Primary Assembly

Range

128702503..128720916

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017014650.2 → XP_016870139.1 serine/threonine-protein kinase N3 isoform X4
XM_047423278.1 → XP_047279234.1 serine/threonine-protein kinase N3 isoform X4
XM_005251946.4 → XP_005252003.1 serine/threonine-protein kinase N3 isoform X2

Conserved Domains (6) summary

PTZ00263
Location:552 → 876

PTZ00263; protein kinase A catalytic subunit; Provisional

cd11622
Location:10 → 75

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11632
Location:94 → 167

HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3

cl00087
Location:167 → 227

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:325 → 407

C2; C2 domain

cl21453
Location:556 → 881

PKc_like; Protein Kinases, catalytic domain
XM_006717080.4 → XP_006717143.1 serine/threonine-protein kinase N3 isoform X1

Conserved Domains (6) summary

cd11622
Location:20 → 85

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11632
Location:104 → 177

HR1_PKN3_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N3

smart00220
Location:566 → 825

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05589
Location:566 → 891

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:177 → 237

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

cl14603
Location:335 → 417

C2; C2 domain
XM_017014649.3 → XP_016870138.1 serine/threonine-protein kinase N3 isoform X3
XM_047423279.1 → XP_047279235.1 serine/threonine-protein kinase N3 isoform X4