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    Mcf2l mcf.2 transforming sequence-like [ Mus musculus (house mouse) ]

    Gene ID: 17207, updated on 27-Nov-2024

    Summary

    Official Symbol
    Mcf2lprovided by MGI
    Official Full Name
    mcf.2 transforming sequence-likeprovided by MGI
    Primary source
    MGI:MGI:103263
    See related
    Ensembl:ENSMUSG00000031442 AllianceGenome:MGI:103263
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Dbs; Ost; mKIAA0362; C130040G20Rik
    Summary
    Enables 1-phosphatidylinositol binding activity and guanyl-nucleotide exchange factor activity. Acts upstream of or within Rho protein signal transduction and positive regulation of transcription by RNA polymerase II. Located in lamellipodium and membrane. Is expressed in several structures, including brain; liver; retina; right atrium; and testis. Orthologous to human MCF2L (MCF.2 cell line derived transforming sequence like). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Broad expression in CNS E18 (RPKM 14.7), frontal lobe adult (RPKM 13.1) and 22 other tissues See more
    Orthologs
    NEW
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    Try the new Transcript table

    Genomic context

    See Mcf2l in Genome Data Viewer
    Location:
    8 A1.1; 8 5.73 cM
    Exon count:
    37
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (12923806..13070502)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (12873806..13020502)

    Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_20748 Neighboring gene predicted gene, 33587 Neighboring gene ATPase, class VI, type 11A Neighboring gene predicted gene 15350 Neighboring gene predicted gene 15353 Neighboring gene STARR-seq mESC enhancer starr_20752 Neighboring gene predicted gene 15351 Neighboring gene STARR-seq mESC enhancer starr_20754 Neighboring gene microRNA 9768 Neighboring gene predicted gene, 53398 Neighboring gene CD24a antigen pseudogene Neighboring gene coagulation factor VII

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Gene trapped (1) 
    • Targeted (1) 

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables 1-phosphatidylinositol binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables guanyl-nucleotide exchange factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables guanyl-nucleotide exchange factor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables guanyl-nucleotide exchange factor activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables phosphatidylinositol binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
     
    located_in cytoplasm ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in endomembrane system ISO
    Inferred from Sequence Orthology
    more info
     
    located_in extrinsic component of cytoplasmic side of plasma membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in lamellipodium IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    guanine nucleotide exchange factor DBS
    Names
    DBL's big sister
    MCF2-transforming sequence-like protein
    Ost gamma
    exchange factor for RhoA and Cdc42
    guanine nucleotide exchange factor OSTIII

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001159485.2NP_001152957.1  guanine nucleotide exchange factor DBS isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR and 5' and 3' coding regions, compared to variant 1, resulting in an isoform (3) that has distinct N- and C-termini and is shorter than isoform 1.
      Source sequence(s)
      AC127308
      Consensus CDS
      CCDS52483.1
      UniProtKB/TrEMBL
      E9PY13, Q003U3
      Related
      ENSMUSP00000106490.3, ENSMUST00000110866.9
      Conserved Domains (6) summary
      cd11857
      Location:10071061
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:24169
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:581758
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:300502
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:765896
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:779893
      PH; PH domain
    2. NM_001159486.2NP_001152958.1  guanine nucleotide exchange factor DBS isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (2) that has a distinct N-terminus and is shorter than isoform 1.
      Source sequence(s)
      AC127308
      Consensus CDS
      CCDS52482.1
      UniProtKB/TrEMBL
      E9PY12, Q003U3
      Related
      ENSMUSP00000106491.3, ENSMUST00000110867.9
      Conserved Domains (6) summary
      cd11857
      Location:10071061
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:24169
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:581758
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:300502
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:765896
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:779893
      PH; PH domain
    3. NM_001371043.1NP_001357972.1  guanine nucleotide exchange factor DBS isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC127308, AC134581
      Consensus CDS
      CCDS90372.1
      UniProtKB/Swiss-Prot
      E9PV70, Q64096
      UniProtKB/TrEMBL
      E9QPM7
      Related
      ENSMUSP00000093108.4, ENSMUST00000095456.10
      Conserved Domains (5) summary
      cd11857
      Location:10591113
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:76221
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:633810
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:352554
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:817948
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    4. NM_001371044.1NP_001357973.1  guanine nucleotide exchange factor DBS isoform 5

      Status: VALIDATED

      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      E9PXE1
      Related
      ENSMUSP00000106503.3, ENSMUST00000110879.9
      Conserved Domains (5) summary
      cd11857
      Location:10541108
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:46191
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:603780
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:322524
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:787918
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    5. NM_001371045.1NP_001357974.1  guanine nucleotide exchange factor DBS isoform 6

      Status: VALIDATED

      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      Q003U3
      Related
      ENSMUSP00000106495.2, ENSMUST00000110871.8
      Conserved Domains (5) summary
      cd11857
      Location:10271081
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:44189
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:601778
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:320522
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:785916
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    6. NM_001371046.1NP_001357975.1  guanine nucleotide exchange factor DBS isoform 7

      Status: VALIDATED

      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      E9PXE1
      Conserved Domains (5) summary
      cd11857
      Location:10251079
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:17162
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:574751
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:293495
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:758889
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    7. NM_001371047.1NP_001357976.1  guanine nucleotide exchange factor DBS isoform 8

      Status: VALIDATED

      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      Q6PDM6
      Conserved Domains (5) summary
      cd11857
      Location:10541108
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:46191
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:603780
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:322524
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:787918
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    8. NM_001371048.1NP_001357977.1  guanine nucleotide exchange factor DBS isoform 9

      Status: VALIDATED

      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      Q6PDM6
      Conserved Domains (5) summary
      cd11857
      Location:10831137
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:44189
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:601778
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:320522
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:785916
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    9. NM_001371049.1NP_001357978.1  guanine nucleotide exchange factor DBS isoform 10

      Status: VALIDATED

      Description
      Transcript Variant: This variant (10) encodes the longest isoform (10).
      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      E9PXE1
      Related
      ENSMUSP00000133577.2, ENSMUST00000145067.8
      Conserved Domains (5) summary
      cd11857
      Location:10821136
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:46191
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:603780
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:322524
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:787918
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    10. NM_001371050.1NP_001357979.1  guanine nucleotide exchange factor DBS isoform 11

      Status: VALIDATED

      Source sequence(s)
      AC127308
      UniProtKB/TrEMBL
      Q003U3
      Related
      ENSMUSP00000096528.4, ENSMUST00000098927.10
      Conserved Domains (5) summary
      cd11857
      Location:10331087
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:50195
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:607784
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:326528
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:791922
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    11. NM_178076.4NP_835177.2  guanine nucleotide exchange factor DBS isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes isoform 1.
      Source sequence(s)
      AC127308
      Consensus CDS
      CCDS40225.2
      UniProtKB/TrEMBL
      E9PXE2, Q6PDM6
      Related
      ENSMUSP00000106500.3, ENSMUST00000110876.9
      Conserved Domains (6) summary
      cd11857
      Location:10851139
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:46191
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:603780
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:322524
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:787918
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:801915
      PH; PH domain

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000074.7 Reference GRCm39 C57BL/6J

      Range
      12923806..13070502
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)