Mlst8 MTOR associated protein, LST8 homolog (S. cerevisiae) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mlst8provided by MGI
Official Full Name: MTOR associated protein, LST8 homolog (S. cerevisiae)provided by MGI
Primary source: MGI:MGI:1929514
See related: Ensembl:ENSMUSG00000024142 AllianceGenome:MGI:1929514
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gbl; 0610033N12Rik
Summary: Predicted to enable protein serine/threonine kinase activator activity. Acts upstream of or within positive regulation of actin filament polymerization and positive regulation of peptidyl-tyrosine phosphorylation. Part of TORC1 complex. Is expressed in central nervous system and retina. Orthologous to human MLST8 (MTOR associated protein, LST8 homolog). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in thymus adult (RPKM 14.9), large intestine adult (RPKM 14.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 A3.3; 17 12.39 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (24692524..24698722, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (24473550..24479114, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the crosstalk between AMPK and mTORC1 signaling is a highly regulated way to control changes in muscle growth and metabolic rate imposed by external cues.
Title: Antagonistic control of muscle cell size by AMPK and mTORC1.
The mutant, Gbeta1(W332A) protein, although inactive, retains its ability to bind Kir3 and prevents the wild type Gbeta from activating the channel.
Title: Dominant negative effects of a Gbeta mutant on G-protein coupled inward rectifier K+ channel.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125846 (e-PCR)
- UniSTS:162589

NoName (e-PCR)
- UniSTS:492327

AA409454 (e-PCR)
- UniSTS:160623

RH124862 (e-PCR)
- UniSTS:161684

NoName (e-PCR)
- UniSTS:235185

AI505104 (e-PCR)
- UniSTS:180976

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein serine/threonine kinase activator activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response	NAS Non-traceable Author Statement more info	PubMed
involved_in TOR signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in TORC1 signaling	IEA Inferred from Electronic Annotation more info
involved_in TORC1 signaling	ISO Inferred from Sequence Orthology more info
involved_in TORC2 signaling	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to hypoxia	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular response to nutrient levels	NAS Non-traceable Author Statement more info	PubMed
involved_in cellular response to osmotic stress	NAS Non-traceable Author Statement more info	PubMed
involved_in cytoskeleton organization	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of apoptotic process	NAS Non-traceable Author Statement more info	PubMed
involved_in negative regulation of autophagy	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of TOR signaling	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of TOR signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell growth	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of glycolytic process	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of lipid biosynthetic process	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of pentose-phosphate shunt	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within positive regulation of peptidyl-tyrosine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of TORC1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TORC1 complex	IDA Inferred from Direct Assay more info	PubMed
part_of TORC1 complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of TORC2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TORC2 complex	ISO Inferred from Sequence Orthology more info	PubMed
part_of TORC2 complex	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in lysosomal membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: target of rapamycin complex subunit LST8

Names: TORC subunit LST8; mammalian lethal with SEC13 protein 8; protein GbetaL; transducin (beta)-like 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001252463.1 → NP_001239392.1 target of rapamycin complex subunit LST8 isoform 1

See identical proteins and their annotated locations for NP_001239392.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the longer isoform (1).

Source sequence(s)

AC154237, AK002751, AK040100

Consensus CDS

CCDS28484.1

UniProtKB/Swiss-Prot

Q8BNG8, Q8C882, Q9DCJ1, Q9JKK6

UniProtKB/TrEMBL

A0A3Q4L2V5

Related

ENSMUSP00000157301.2, ENSMUST00000234335.2

Conserved Domains (3) summary

COG2319
Location:10 → 305

WD40; WD40 repeat [General function prediction only]

cd00200
Location:14 → 297

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:47 → 83

7WD40; WD40 repeat [structural motif]
NM_001252464.1 → NP_001239393.1 target of rapamycin complex subunit LST8 isoform 1

See identical proteins and their annotated locations for NP_001239393.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the longer isoform (1).

Source sequence(s)

AC154237, AK040100, AK083731, CF750475

Consensus CDS

CCDS28484.1

UniProtKB/Swiss-Prot

Q8BNG8, Q8C882, Q9DCJ1, Q9JKK6

UniProtKB/TrEMBL

A0A3Q4L2V5

Related

ENSMUSP00000136287.2, ENSMUST00000179163.3

Conserved Domains (3) summary

COG2319
Location:10 → 305

WD40; WD40 repeat [General function prediction only]

cd00200
Location:14 → 297

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:47 → 83

7WD40; WD40 repeat [structural motif]
NM_001252465.1 → NP_001239394.1 target of rapamycin complex subunit LST8 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (4) lacks two internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

AC154237, AK040100, BM947104

Consensus CDS

CCDS89006.1

UniProtKB/TrEMBL

A0A3Q4EC26, A0A3Q4L2V5

Related

ENSMUSP00000157225.2, ENSMUST00000234543.2

Conserved Domains (3) summary

COG2319
Location:1 → 257

WD40; WD40 repeat [General function prediction only]

sd00039
Location:23 → 61

7WD40; WD40 repeat [structural motif]

cl02567
Location:5 → 231

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
NM_019988.5 → NP_064372.2 target of rapamycin complex subunit LST8 isoform 1

See identical proteins and their annotated locations for NP_064372.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the longer isoform (1).

Source sequence(s)

AC154237, AK040100

Consensus CDS

CCDS28484.1

UniProtKB/Swiss-Prot

Q8BNG8, Q8C882, Q9DCJ1, Q9JKK6

UniProtKB/TrEMBL

A0A3Q4L2V5

Related

ENSMUSP00000065004.7, ENSMUST00000070888.14

Conserved Domains (3) summary

COG2319
Location:10 → 305

WD40; WD40 repeat [General function prediction only]

cd00200
Location:14 → 297

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:47 → 83

7WD40; WD40 repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

24692524..24698722 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017317586.2 → XP_017173075.1 target of rapamycin complex subunit LST8 isoform X1

UniProtKB/TrEMBL

A0A3Q4L2V5

Conserved Domains (2) summary

cd00200
Location:14 → 299

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:47 → 83

7WD40; WD40 repeat [structural motif]
XM_006524679.5 → XP_006524742.1 target of rapamycin complex subunit LST8 isoform X3

UniProtKB/TrEMBL

A0A3Q4L2V5

Conserved Domains (2) summary

cd00200
Location:14 → 296

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:46 → 82

7WD40; WD40 repeat [structural motif]
XM_006524678.3 → XP_006524741.1 target of rapamycin complex subunit LST8 isoform X2

UniProtKB/TrEMBL

A0A3Q4L2V5

Conserved Domains (2) summary

cd00200
Location:14 → 298

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:46 → 82

7WD40; WD40 repeat [structural motif]
XM_030249921.1 → XP_030105781.1 target of rapamycin complex subunit LST8 isoform X2

UniProtKB/TrEMBL

A0A3Q4L2V5

Conserved Domains (2) summary

cd00200
Location:14 → 298

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:46 → 82

7WD40; WD40 repeat [structural motif]
XM_017317587.1 → XP_017173076.1 target of rapamycin complex subunit LST8 isoform X1

UniProtKB/TrEMBL

A0A3Q4L2V5

Conserved Domains (2) summary

cd00200
Location:14 → 299

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:47 → 83

7WD40; WD40 repeat [structural motif]
XM_030249920.2 → XP_030105780.1 target of rapamycin complex subunit LST8 isoform X1

UniProtKB/TrEMBL

A0A3Q4L2V5

Conserved Domains (2) summary

cd00200
Location:14 → 299

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:47 → 83

7WD40; WD40 repeat [structural motif]