fbf-1 Fem-3 mRNA-binding factor 1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: fbf-1
Official Full Name: Fem-3 mRNA-binding factor 1
Primary source: WormBase:WBGene00001401
Locus tag: CELE_H12I13.4
See related: AllianceGenome:WB:WBGene00001401
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables mRNA 3'-UTR binding activity. Involved in several processes, including germline cell cycle switching, mitotic to meiotic cell cycle; olfactory learning; and regulation of gene expression. Located in germ cell nucleus and perinuclear region of cytoplasm. Is expressed in germ line. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See fbf-1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 8

Sequence:: Chromosome: II; NC_003280.10 (6078263..6080843, complement)

Chromosome II - NC_003280.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003280.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.
Title: COP9 signalosome component CSN-5 stabilizes PUF proteins FBF-1 and FBF-2 in Caenorhabditis elegans germline stem and progenitor cells.
Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2.
Title: Antagonistic control of Caenorhabditis elegans germline stem cell proliferation and differentiation by PUF proteins FBF-1 and FBF-2.
Authors conclude that a "PUF hub," comprising four PUF proteins and two PUF partners, constitutes the intrinsic self-renewal node of the C. elegans GSC RNA regulatory network.
Title: A PUF Hub Drives Self-Renewal in Caenorhabditis elegans Germline Stem Cells.
Block III (21 RNAs) was enriched for RNA-binding proteins, including previously validated FBF targets gld-1 and htp-1 We suggest that Block I RNAs belong to the FBF network for spermatogenesis, and that Blocks II and III are associated with stem cell functions.
Title: Toward Identifying Subnetworks from FBF Binding Landscapes in Caenorhabditis Spermatogenic or Oogenic Germlines.
It was concluded that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery.
Title: Transition fibre protein FBF1 is required for the ciliary entry of assembled intraflagellar transport complexes.
Data indicate that a 10-amino-acid region in germline development defective-3 (GLD-3) is required for fem-3 binding factor (FBF) binding, while a 7-amino-acid loop in FBF between PUF repeats 7 and 8 is necessary for GLD-3 binding.
Title: A protein.protein interaction platform involved in recruitment of GLD-3 to the FBF.fem-3 mRNA complex.
Data show that CPB-1 (cytoplasmic polyadenylation element binding protein homolog-1) alters the affinity of FBF (fem-3 mRNA binding factor) for specific RNA sequences.
Title: Biochemical characterization of the Caenorhabditis elegans FBF.CPB-1 translational regulation complex identifies conserved protein interaction hotspots.
Here, the authors report that FBF/Pumilio, a conserved RNA-binding protein, promotes self-renewal of germline stem cells by repressing CKI-2(Cip/Kip), a Cyclin E/Cdk2 inhibitor.
Title: FBF represses the Cip/Kip cell-cycle inhibitor CKI-2 to promote self-renewal of germline stem cells in C. elegans.
The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
Title: The Puf RNA-binding proteins FBF-1 and FBF-2 inhibit the expression of synaptonemal complex proteins in germline stem cells.
FBF can affect polyadenylation either negatively by its CCF-1 interaction or positively by its GLD-2 interaction in control of GLD1 expression.
Title: FBF and its dual control of gld-1 expression in the Caenorhabditis elegans germline.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

H12I13.a (e-PCR)
- UniSTS:322575

H12I13.e (e-PCR)
- UniSTS:322579

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in germline cell cycle switching, mitotic to meiotic cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in olfactory learning	IGI Inferred from Genetic Interaction more info	PubMed
involved_in olfactory learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-transcriptional regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in post-transcriptional regulation of gene expression	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sex differentiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in germ cell nucleus	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Fem-3 mRNA-binding factor 1

NP_495216.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003280.10 Reference assembly

Range

6078263..6080843 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_062815.8 → NP_495216.1 Fem-3 mRNA-binding factor 1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_495216.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9N5M6

Conserved Domains (1) summary

cl21719
Location:180 → 560

Pumilio; Pumilio-family RNA binding domain