Rnf216 ring finger protein 216 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rnf216provided by MGI
Official Full Name: ring finger protein 216provided by MGI
Primary source: MGI:MGI:1344349
See related: Ensembl:ENSMUSG00000045078 AllianceGenome:MGI:1344349
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: UIP83; TRIAD3; Ubce7ip1; 2810055G22Rik; F830018F18Rik
Summary: Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in several processes, including protein K48-linked ubiquitination; protein catabolic process, modulating synaptic transmission; and regulation of postsynaptic neurotransmitter receptor internalization. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in several cellular components, including Schaffer collateral - CA1 synapse; glutamatergic synapse; and postsynaptic endocytic zone. Human ortholog(s) of this gene implicated in Gordon Holmes syndrome. Orthologous to human RNF216 (ring finger protein 216). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in testis adult (RPKM 22.7), adrenal adult (RPKM 18.3) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 81.86 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (142976648..143098793, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (142990893..143113041, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9 impairs mitochondrial bioenergetics and exacerbates diabetic cardiomyopathy.
Title: Triad3A-Mediated K48-Linked ubiquitination and degradation of TLR9 impairs mitochondrial bioenergetics and exacerbates diabetic cardiomyopathy.
RNF216 affects the stability of STAU2 in the hypothalamus.
Title: RNF216 affects the stability of STAU2 in the hypothalamus.
RNF216 regulates meiosis and PKA stability in the testes.
Title: RNF216 regulates meiosis and PKA stability in the testes.
Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis.
Title: Triad3A displays a critical role in suppression of cerebral ischemic/reperfusion (I/R) injury by regulating necroptosis.
Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9.
Title: Triad3A attenuates pathological cardiac hypertrophy involving the augmentation of ubiquitination-mediated degradation of TLR4 and TLR9.
An essential function of RNF216 in spermatogenesis and male fertility and suggest a critical role for RNF216 in human gonadal development.
Title: RNF216 is essential for spermatogenesis and male fertility†.
Triad3a promotes the negative feedback loop independently of typical RipK1 ubiquitin editing enzymes
Title: Triad3a induces the degradation of early necrosome to limit RipK1-dependent cytokine production and necroptosis.
Low RNF216 expression is associated with obesity-induced inflammation.
Title: Green Tea Polyphenol Epigallocatechin-3-gallate Suppresses Toll-like Receptor 4 Expression via Up-regulation of E3 Ubiquitin-protein Ligase RNF216.
missense mutations in TRIAD3 abolished the interaction of TRIAD3A with Arc, disrupting Arc ubiquitination, and consequently Arc degradation.
Title: TRIAD3/RNF216 mutations associated with Gordon Holmes syndrome lead to synaptic and cognitive impairments via Arc misregulation.
identifies a novel negative regulator of autophagy and suggests that RNF216 may be a target for treatment of inflammatory diseases
Title: Regulation of autophagy by E3 ubiquitin ligase RNF216 through BECN1 ubiquitination.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

C86502 (e-PCR)
- UniSTS:207908

AU015632 (e-PCR)
- UniSTS:167461

D5Mit62 (e-PCR), detects polymorphism
- UniSTS:130600

AU015632 (e-PCR)
- UniSTS:179311

AI647468 (e-PCR)
- UniSTS:179313

D1S3690 (e-PCR)
- UniSTS:474268

RH136404 (e-PCR)
- UniSTS:210394

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein K48-linked ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein K48-linked ubiquitination	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein catabolic process, modulating synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in protein catabolic process, modulating synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in regulation of defense response to virus by host	ISO Inferred from Sequence Orthology more info
involved_in regulation of defense response to virus by host	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of interferon-beta production	ISO Inferred from Sequence Orthology more info
involved_in regulation of interferon-beta production	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of postsynaptic neurotransmitter receptor internalization	IEA Inferred from Electronic Annotation more info
involved_in regulation of postsynaptic neurotransmitter receptor internalization	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Schaffer collateral - CA1 synapse	IEA Inferred from Electronic Annotation more info
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
located_in clathrin-coated vesicle	IEA Inferred from Electronic Annotation more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in postsynaptic endocytic zone	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic endocytic zone	ISO Inferred from Sequence Orthology more info
located_in presynapse	IEA Inferred from Electronic Annotation more info
is_active_in presynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RNF216

Names: E3 ubiquitin ligase; RING-type E3 ubiquitin transferase RNF216; triad domain-containing protein 3; ubcM4-interacting protein 83; ubiquitin-conjugating enzyme 7-interacting protein 1

NP_001346780.1

EC 2.3.2.27

NP_542128.2

EC 2.3.2.27

NP_996993.1

EC 2.3.2.27

XP_006504707.1

EC 2.3.2.27

XP_006504708.1

EC 2.3.2.27

XP_006504709.1

EC 2.3.2.27

XP_017176080.1

EC 2.3.2.27

XP_017176081.1

EC 2.3.2.27

XP_017176082.1

EC 2.3.2.27

XP_030109902.1

EC 2.3.2.27

XP_036020615.1

EC 2.3.2.27

XP_036020616.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001359851.1 → NP_001346780.1 E3 ubiquitin-protein ligase RNF216 isoform C

Status: VALIDATED

Description

Transcript Variant: This variant (C) differs in the 5' UTR and coding sequence compared to variant B. The resulting isoform (C) is shorter at the N-terminus compared to isoform B.

Source sequence(s)

AC113281

Conserved Domains (1) summary

cd16630
Location:310 → 366

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
NM_080561.4 → NP_542128.2 E3 ubiquitin-protein ligase RNF216 isoform A

See identical proteins and their annotated locations for NP_542128.2

Status: VALIDATED

Description

Transcript Variant: This variant (A) uses an alternate in-frame splice junction compared to variant B. The resulting isoform (A) has the same N- and C-termini but is shorter compared to isoform B.

Source sequence(s)

BB854046, BC065066

Consensus CDS

CCDS80455.1

UniProtKB/Swiss-Prot

P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3

Related

ENSMUSP00000052563.10, ENSMUST00000053498.13

Conserved Domains (2) summary

cd16630
Location:501 → 557

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:643 → 693

IBR; IBR domain, a half RING-finger domain
NM_207110.1 → NP_996993.1 E3 ubiquitin-protein ligase RNF216 isoform B

See identical proteins and their annotated locations for NP_996993.1

Status: VALIDATED

Description

Transcript Variant: This variant (B) represents the longest transcript and encodes the longest isoform (B).

Source sequence(s)

AC113281, AK147861, BB854046, BC065066

Consensus CDS

CCDS57400.1

UniProtKB/Swiss-Prot

P58283

Related

ENSMUSP00000143705.2, ENSMUST00000200607.5

Conserved Domains (2) summary

cd16630
Location:558 → 614

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:700 → 750

IBR; IBR domain, a half RING-finger domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

142976648..143098793 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030254042.1 → XP_030109902.1 E3 ubiquitin-protein ligase RNF216 isoform X4

Conserved Domains (2) summary

cd16630
Location:416 → 472

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:558 → 608

IBR; IBR domain, a half RING-finger domain
XM_006504646.4 → XP_006504709.1 E3 ubiquitin-protein ligase RNF216 isoform X3

Conserved Domains (2) summary

cd16630
Location:473 → 529

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:615 → 665

IBR; IBR domain, a half RING-finger domain
XM_006504644.5 → XP_006504707.1 E3 ubiquitin-protein ligase RNF216 isoform X1

See identical proteins and their annotated locations for XP_006504707.1

UniProtKB/Swiss-Prot

P58283

Conserved Domains (2) summary

cd16630
Location:558 → 614

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:700 → 750

IBR; IBR domain, a half RING-finger domain
XM_006504645.5 → XP_006504708.1 E3 ubiquitin-protein ligase RNF216 isoform X2

See identical proteins and their annotated locations for XP_006504708.1

UniProtKB/Swiss-Prot

P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3

Conserved Domains (2) summary

cd16630
Location:501 → 557

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:643 → 693

IBR; IBR domain, a half RING-finger domain
XM_017320593.3 → XP_017176082.1 E3 ubiquitin-protein ligase RNF216 isoform X5

Conserved Domains (1) summary

cd16630
Location:310 → 366

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
XM_017320592.3 → XP_017176081.1 E3 ubiquitin-protein ligase RNF216 isoform X5

Conserved Domains (1) summary

cd16630
Location:310 → 366

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins
XM_036164722.1 → XP_036020615.1 E3 ubiquitin-protein ligase RNF216 isoform X1

Conserved Domains (2) summary

cd16630
Location:558 → 614

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:700 → 750

IBR; IBR domain, a half RING-finger domain
XM_017320591.3 → XP_017176080.1 E3 ubiquitin-protein ligase RNF216 isoform X1

UniProtKB/Swiss-Prot

P58283

Conserved Domains (2) summary

cd16630
Location:558 → 614

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:700 → 750

IBR; IBR domain, a half RING-finger domain
XM_036164723.1 → XP_036020616.1 E3 ubiquitin-protein ligase RNF216 isoform X2

UniProtKB/Swiss-Prot

P58283, Q3U493, Q3UE56, Q3UGM3, Q68FN0, Q6P1H8, Q6PWY5, Q8BN27, Q8C1U3

Conserved Domains (2) summary

cd16630
Location:501 → 557

RING-HC_RBR_RNF216; RING finger, HC subclass, found in RING finger protein 216 (RNF216) and similar proteins

pfam01485
Location:643 → 693

IBR; IBR domain, a half RING-finger domain