Cul5 cullin 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cul5provided by MGI
Official Full Name: cullin 5provided by MGI
Primary source: MGI:MGI:1922967
See related: Ensembl:ENSMUSG00000032030 AllianceGenome:MGI:1922967
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: VACM1; VACM-1; 4921514I20Rik; 8430423K24Rik; C030032G03Rik; C330021I08Rik
Summary: Enables ubiquitin ligase complex scaffold activity. Involved in proteasome-mediated ubiquitin-dependent protein catabolic process and regulation of neuron migration. Acts upstream of with a negative effect on reelin-mediated signaling pathway. Acts upstream of or within cerebral cortex radially oriented cell migration; protein ubiquitination; and radial glia guided migration of Purkinje cell. Part of Cul5-RING ubiquitin ligase complex. Is expressed in several structures, including brain; eye; genitourinary system; gut; and limb bud. Orthologous to human CUL5 (cullin 5). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 7.1), CNS E18 (RPKM 5.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A5.3; 9 29.12 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (53525881..53578807, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (53614581..53667507, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8[+] T cells.
Title: The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8(+) T cells.
Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity.
Title: Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity.
The ubiquitin ligase Cul5 regulates CD4(+) T cell fate choice and allergic inflammation.
Title: The ubiquitin ligase Cul5 regulates CD4(+) T cell fate choice and allergic inflammation.
work identifies the essential role of CUL5- and SOCS3-mediated integrin beta1 turnover in controlling small-cell lung cancer metastasis, which might have therapeutic implications.
Title: Cullin5 deficiency promotes small-cell lung cancer metastasis by stabilizing integrin β1.
r results show that Cul5, but not Rbx2, is required during early embryogenesis and suggests that Cul5 has Rbx2-independent functions in early development.
Title: Comparative Analysis of cul5 and rbx2 Expression in the Developing and Adult Murine Brain and Their Essentiality During Mouse Embryogenesis.
neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 is required for its interaction with TRAF6.
Title: Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.
Our results show that endogenous Cul5 suppresses epithelial cell transformation by several pathways, including inhibition of Src-Cas-induced ruffling through SOCS6
Title: Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.
The expression of cullin5 on retinal cells and reduction in the percentage of dead retinal cells induced by L-glutamate suggest that cullin5 has neuroprotective properties in retinal cells.
Title: Cullin5 reduces retinal cell death induced by glutamate toxicity.
SOCS box acts as an independent binding domain capable of recruiting elonginBC and cullin5 to promote E3 ligase formation.
Title: The SOCS box domain of SOCS3: structure and interaction with the elonginBC-cullin5 ubiquitin ligase.
The role of Cul5 is to degrade disabled homolog 1 (Dab1) and thus control neuron migration speed and neuron insertion at the top of the cortical plate.
Title: Cullin 5 regulates cortical layering by modulating the speed and duration of Dab1-dependent neuronal migration.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (2)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:236129

AI852817 (e-PCR)
- UniSTS:165630

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin ligase complex scaffold activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cerebral cortex radially oriented cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein ubiquitination	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within radial glia guided migration of Purkinje cell	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_negative_effect reelin-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
involved_in regulation of neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cul5-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul5-RING ubiquitin ligase complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of Cul5-RING ubiquitin ligase complex	ISO Inferred from Sequence Orthology more info
part_of SCF ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in site of DNA damage	ISO Inferred from Sequence Orthology more info
located_in site of DNA damage	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: cullin-5

Names: CUL-5; vasopressin-activated calcium-mobilizing receptor protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001161618.2 → NP_001155090.2 cullin-5 isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

Source sequence(s)

AC158384

Consensus CDS

CCDS52798.1

UniProtKB/TrEMBL

E9PV12, G3X914

Related

ENSMUSP00000133144.3, ENSMUST00000166367.8

Conserved Domains (2) summary

pfam00888
Location:19 → 645

Cullin; Cullin family

pfam10557
Location:686 → 745

Cullin_Nedd8; Cullin protein neddylation domain
NM_027807.4 → NP_082083.3 cullin-5 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC158384

Consensus CDS

CCDS40638.2

UniProtKB/Swiss-Prot

Q8BMQ6, Q8BV53, Q8C098, Q9D5V5

UniProtKB/TrEMBL

G3X914, Q2M4H5

Related

ENSMUSP00000034529.8, ENSMUST00000034529.14

Conserved Domains (2) summary

pfam00888
Location:19 → 672

Cullin; Cullin family

pfam10557
Location:713 → 772

Cullin_Nedd8; Cullin protein neddylation domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

53525881..53578807 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017313680.3 → XP_017169169.1 cullin-5 isoform X1

UniProtKB/TrEMBL

E9Q6Z0

Related

ENSMUSP00000113490.2, ENSMUST00000120122.8

Conserved Domains (2) summary

pfam00888
Location:6 → 543

Cullin; Cullin family

pfam10557
Location:584 → 641

Cullin_Nedd8; Cullin protein neddylation domain