Fxyd1 FXYD domain-containing ion transport regulator 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Fxyd1provided by RGD
Official Full Name: FXYD domain-containing ion transport regulator 1provided by RGD
Primary source: RGD:69306
See related: EnsemblRapid:ENSRNOG00000021079 AllianceGenome:RGD:69306
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Plm
Summary: This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. [provided by RefSeq, Jul 2008]
Expression: Biased expression in Heart (RPKM 972.9), Muscle (RPKM 830.7) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q21

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (95414556..95418645, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (86287163..86291478, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (89484197..89488279, complement)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest Ncx1 (sodium-calcium exchanger 1) is substrate-specifying Pp1c (protein phosphatase 1c) regulator protein, regulating Ncx1 activity via Ser-68-Plm dephosphorylation in cardiomyocytes; Plm/Ncx1/Pp1c complex is up-regulated in heart failure.
Title: Protein Phosphatase 1c Associated with the Cardiac Sodium Calcium Exchanger 1 Regulates Its Activity by Dephosphorylating Serine 68-phosphorylated Phospholemman.
PLM exists as a pump-inhibiting monomer and an unassociated oligomer.
Title: A separate pool of cardiac phospholemman that does not regulate or associate with the sodium pump: multimers of phospholemman in ventricular muscle.
Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.
Title: Chronic nicotine modifies skeletal muscle Na,K-ATPase activity through its interaction with the nicotinic acetylcholine receptor and phospholemman.
role of protein phosphatase-1 on phospholemman phosphorylation and Na/K-ATPase activity in cardiomyocytes
Title: Phospholemman-dependent regulation of the cardiac Na/K-ATPase activity is modulated by inhibitor-1 sensitive type-1 phosphatase.
palmitoylation of PLM controls its turnover, and palmitoylated PLM inhibits the sodium pump.
Title: The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation.
Report altered expression of Na(+)-K(+) pump subunits, FXYD1 and NHE1 depending on training status, intensity and muscle type.
Title: Exercise-induced regulation of muscular Na+-K+ pump, FXYD1, and NHE1 mRNA and protein expression: importance of training status, intensity, and muscle type.
Conclude that threonine 69 is a previously undescribed protein kinase c phosphorylation site in FXYD1.
Title: FXYD1 phosphorylation in vitro and in adult rat cardiac myocytes: threonine 69 is a novel substrate for protein kinase C.
These results suggest that FXYD1 contributes to facilitating the advent of puberty by maintaining GnRH neuronal excitability to incoming transsynaptic stimulatory inputs.
Title: FXYD1, a modulator of Na,K-ATPase activity, facilitates female sexual development by maintaining gonadotrophin-releasing hormone neuronal excitability.
PLM interacts with the intracellular loop of NCX1, most likely at residues 218-358
Title: Cytoplasmic tail of phospholemman interacts with the intracellular loop of the cardiac Na+/Ca2+ exchanger.
The functional consequences of direct association of PLM with NCX1 was the inhibition of NCX1 activity.
Title: Identification of an endogenous inhibitor of the cardiac Na+/Ca2+ exchanger, phospholemman.

Submit: New GeneRIF Correction See all GeneRIFs (16)

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATPase binding	IDA Inferred from Direct Assay more info	PubMed
enables ion channel regulator activity	IEA Inferred from Electronic Annotation more info
enables myosin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sodium channel regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables sodium channel regulator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in negative regulation of protein glutathionylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein glutathionylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of inorganic anion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of organic acid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of sodium ion export across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of sodium ion export across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sodium ion export across plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
involved_in potassium ion transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of P-type sodium:potassium-exchanging transporter activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of cardiac muscle cell contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cardiac muscle cell membrane potential	ISO Inferred from Sequence Orthology more info
involved_in regulation of cytosolic calcium ion concentration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of sodium ion transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
involved_in sodium ion transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in T-tubule	IDA Inferred from Direct Assay more info	PubMed
located_in T-tubule	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in caveola	IDA Inferred from Direct Assay more info	PubMed
located_in caveola	IEA Inferred from Electronic Annotation more info
located_in intercalated disc	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
part_of sodium:potassium-exchanging ATPase complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: phospholemman

Names: sodium/potassium-transporting ATPase subunit FXYD1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031648.2 → NP_113836.2 phospholemman precursor

Status: VALIDATED

Source sequence(s)

CB712570, FM109156

UniProtKB/TrEMBL

A0A0H2UHS8, A0A8I5ZW12, A6JA55

Related

ENSRNOP00000028624.2, ENSRNOT00000028624.6

Conserved Domains (1) summary

pfam02038
Location:27 → 72

ATP1G1_PLM_MAT8; ATP1G1/PLM/MAT8 family

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

95414556..95418645 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039088782.2 → XP_038944710.1 phospholemman isoform X4

Conserved Domains (1) summary

cl40453
Location:63 → 101

FXYD; phenylalanine-X-tyrosine-aspartate (FXYD) family
XM_063272192.1 → XP_063128262.1 phospholemman isoform X6
XM_017589631.3 → XP_017445120.1 phospholemman isoform X5

UniProtKB/Swiss-Prot

O08589

UniProtKB/TrEMBL

A0A8I5ZW12, A6JA62

Conserved Domains (1) summary

pfam02038
Location:24 → 69

ATP1G1_PLM_MAT8; ATP1G1/PLM/MAT8 family
XM_039088778.2 → XP_038944706.1 phospholemman isoform X2
XM_063272174.1 → XP_063128244.1 phospholemman isoform X1

UniProtKB/TrEMBL

A0A8I6G4X1
XM_017589629.3 → XP_017445118.1 phospholemman isoform X1

UniProtKB/TrEMBL

A0A8I6G4X1
XM_063272185.1 → XP_063128255.1 phospholemman isoform X5

UniProtKB/Swiss-Prot

O08589

UniProtKB/TrEMBL

A6JA62
XM_006228839.5 → XP_006228901.1 phospholemman isoform X5

UniProtKB/Swiss-Prot

O08589

UniProtKB/TrEMBL

A0A8I5ZW12, A6JA62

Related

ENSRNOP00000083421.2, ENSRNOT00000102674.2

Conserved Domains (1) summary

pfam02038
Location:24 → 69

ATP1G1_PLM_MAT8; ATP1G1/PLM/MAT8 family
XM_017589630.3 → XP_017445119.1 phospholemman isoform X3

UniProtKB/TrEMBL

A0A8I5ZW12, A6JA56

Conserved Domains (1) summary

pfam02038
Location:46 → 91

ATP1G1_PLM_MAT8; ATP1G1/PLM/MAT8 family
XM_063272182.1 → XP_063128252.1 phospholemman isoform X5

UniProtKB/Swiss-Prot

O08589

UniProtKB/TrEMBL

A6JA62