Slc22a5 solute carrier family 22 member 5 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc22a5provided by RGD
Official Full Name: solute carrier family 22 member 5provided by RGD
Primary source: RGD:3702
See related: EnsemblRapid:ENSRNOG00000008432 AllianceGenome:RGD:3702
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CT1; OCTN2; UST2r
Summary: Enables (R)-carnitine transmembrane transporter activity. Involved in (R)-carnitine transmembrane transport. Located in basolateral plasma membrane and cytosol. Biomarker of congestive heart failure. Human ortholog(s) of this gene implicated in Crohn's disease; cardiomyopathy; inherited metabolic disorder; and systemic primary carnitine deficiency disease. Orthologous to human SLC22A5 (solute carrier family 22 member 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Kidney (RPKM 643.8), Testes (RPKM 98.3) and 7 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q22

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (38509072..38536240, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (38008303..38035474, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (39201101..39228090, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

OCTN2 plays a positive role in epididymal epithelial cells, and might be useful in the clinical treatment of male infertility by serving as a key regulatory factor.
Title: Carnitine/organic cation transporter 2 (OCTN2) contributes to rat epididymal epithelial cell growth and proliferation.
PKC activation resulted in an augmented Octn2 presence in cholesterol/sphingolipid-rich microdomains of plasma membrane (rafts) and increased co-precipitation of Octn2 with raft-proteins, caveolin-1 and flotillin-1.
Title: Caveolin-1--a novel interacting partner of organic cation/carnitine transporter (Octn2): effect of protein kinase C on this interaction in rat astrocytes.
HgCl2 and methylmercury inhibit OCTN2 carnitine transporter in the proteoliposome experimental model.
Title: Inhibition of the OCTN2 carnitine transporter by HgCl2 and methylmercury in the proteoliposome experimental model: insights in the mechanism of toxicity.
The present study showed that muscle contraction facilitated carnitine uptake in skeletal muscles, possibly via the contraction-induced translocation of its specific transporter OCTN2 to the plasma membrane.
Title: Muscle contraction increases carnitine uptake via translocation of OCTN2.
Data suggests that Slc22a5 plays a role in kidney in reabsorption of carnitine from urine; with training in physical activity, expression of Slc22a5 mRNA is up-regulated in kidney.
Title: The effects of exercise training on γ-butyrobetaine hydroxylase and novel organic cation transporter-2 gene expression in the rat.
Lipopolysaccharide-induced inflammation generally downregulates glucose, fatty acid, and L-carnitine transporter expression; whether this leads to reductions in substrate availability to epithelial cells requires investigation.
Title: LPS-induced inflammation downregulates mammary gland glucose, fatty acid, and L-carnitine transporter expression at different lactation stages.
OCTN2 is functionally expressed in skeletal muscles and is involved in the import of carnitine for fatty acid oxidation, especially in highly oxidative muscles.
Title: OCTN2 is associated with carnitine transport capacity of rat skeletal muscles.
Present study demonstrates expression of octn2 and octn3 genes coding high affinity carnitine transporters, as well as presence of both proteins in neurons obtained from suckling and adult rats.
Title: High affinity carnitine transporters from OCTN family in neural cells.
This is the first report showing a functional apical OCTN2 in the renal medulla and a functional apical OCTN3 in both renal cortex and medulla.
Title: Expression of OCTN2 and OCTN3 in the apical membrane of rat renal cortex and medulla.
OCTN2 is involved in the transport of acetyl-L-carnitine from the circulating blood to the retina across the inner BRB.
Title: Involvement of OCTN2 in the transport of acetyl-L-carnitine across the inner blood-retinal barrier.

Submit: New GeneRIF Correction See all GeneRIFs (18)

General gene information

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Markers

Slc22a5 (e-PCR), detects polymorphism
- UniSTS:463372

AU047818 (e-PCR)
- UniSTS:110144

RH131079 (e-PCR)
- UniSTS:214362

RH137025 (e-PCR)
- UniSTS:219114

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables (R)-carnitine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
NOT enables (R)-carnitine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables (R)-carnitine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables (R)-carnitine transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables PDZ domain binding	ISO Inferred from Sequence Orthology more info
enables amino-acid betaine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables amino-acid betaine transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables carnitine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables carnitine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables carnitine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables quaternary ammonium group transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables quaternary ammonium group transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables quaternary ammonium group transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables quaternary ammonium group transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables symporter activity	IEA Inferred from Electronic Annotation more info
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in (R)-carnitine transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in (R)-carnitine transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in (R)-carnitine transmembrane transport	ISO Inferred from Sequence Orthology more info
NOT involved_in (R)-carnitine transport	ISO Inferred from Sequence Orthology more info
involved_in (R)-carnitine transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within adult heart development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within carnitine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in carnitine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in carnitine transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within carnitine transport	ISO Inferred from Sequence Orthology more info
involved_in carnitine transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of localization in cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within locomotory behavior	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mitochondrion organization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intestinal epithelial structure maintenance	ISO Inferred from Sequence Orthology more info
involved_in quaternary ammonium group transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in quaternary ammonium group transport	IDA Inferred from Direct Assay more info	PubMed
involved_in quaternary ammonium group transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within quaternary ammonium group transport	ISO Inferred from Sequence Orthology more info
involved_in quaternary ammonium group transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within reproductive structure development	ISO Inferred from Sequence Orthology more info
involved_in response to tumor necrosis factor	ISO Inferred from Sequence Orthology more info
involved_in response to tumor necrosis factor	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to type II interferon	ISO Inferred from Sequence Orthology more info
involved_in response to type II interferon	ISS Inferred from Sequence or Structural Similarity more info
involved_in sodium ion transport	IEA Inferred from Electronic Annotation more info
involved_in sodium-dependent organic cation transport	ISO Inferred from Sequence Orthology more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in transport across blood-brain barrier	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in brush border membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
NOT located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: organic cation/carnitine transporter 2

Names: high-affinity carnitine transporter; high-affinity sodium-dependent carnitine cotransporter; integral membrane transport protein; solute carrier family 22 (organic cation transporter), member 5; solute carrier family 22 (organic cation/carnitine transporter), member 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019269.2 → NP_062142.1 organic cation/carnitine transporter 2

See identical proteins and their annotated locations for NP_062142.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

O70594, Q9QWL0

UniProtKB/TrEMBL

A6HEF4, B2GUV4

Related

ENSRNOP00000011340.5, ENSRNOT00000011340.6

Conserved Domains (2) summary

cd06174
Location:123 → 510

MFS; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of ...

TIGR00898
Location:12 → 520

2A0119; cation transport protein

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

38509072..38536240 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039085795.2 → XP_038941723.1 organic cation/carnitine transporter 2 isoform X3

UniProtKB/TrEMBL

A6HEF5

Conserved Domains (1) summary

TIGR00898
Location:1 → 277

2A0119; cation transport protein
XM_039085794.1 → XP_038941722.1 organic cation/carnitine transporter 2 isoform X3

UniProtKB/TrEMBL

A6HEF5

Conserved Domains (1) summary

TIGR00898
Location:1 → 277

2A0119; cation transport protein
XM_039085793.1 → XP_038941721.1 organic cation/carnitine transporter 2 isoform X3

UniProtKB/TrEMBL

A6HEF5

Conserved Domains (1) summary

TIGR00898
Location:1 → 277

2A0119; cation transport protein
XM_039085791.2 → XP_038941719.1 organic cation/carnitine transporter 2 isoform X1

Conserved Domains (1) summary

TIGR00898
Location:12 → 322

2A0119; cation transport protein
XM_039085792.2 → XP_038941720.1 organic cation/carnitine transporter 2 isoform X2

Conserved Domains (1) summary

TIGR00898
Location:12 → 317

2A0119; cation transport protein