Mical Molecule interacting with CasL [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Micalprovided by FlyBase
Official Full Name: Molecule interacting with CasLprovided by FlyBase
Primary source: FLYBASE:FBgn0053208
Locus tag: Dmel_CG33208
See related: AllianceGenome:FB:FBgn0053208
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG11685; CG11687; CG18484; CG18667; CG18668; CG33186; CG33190; CG33208; Dmel\CG33208; mdcds_49226; mical; MICAL; milch
Summary: Enables F-actin monooxygenase activity; FAD binding activity; and actin binding activity. Involved in several processes, including actin cytoskeleton organization; neuron development; and synapse assembly involved in innervation. Located in terminal bouton. Colocalizes with plasma membrane. Is expressed in several structures, including adult head; embryonic/larval midgut; embryonic/larval midgut primordium; larval muscle system; and larval somatic muscle cell. Orthologous to human MICAL2 (microtubule associated monooxygenase, calponin and LIM domain containing 2) and MICAL3 (microtubule associated monooxygenase, calponin and LIM domain containing 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 85F5-85F7; 3-49 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (10001554..10042895, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (5827276..5868617, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mical modulates Tau toxicity via cysteine oxidation in vivo.
Title: Mical modulates Tau toxicity via cysteine oxidation in vivo.
Profilin and Mical combine to impair F-actin assembly and promote disassembly and remodeling.
Title: Profilin and Mical combine to impair F-actin assembly and promote disassembly and remodeling.
Mical and cofilin, therefore, form a redox-dependent synergistic pair that promotes F-actin instability by rapidly dismantling F-actin and generating post-translationally modified actin that has altered assembly properties
Title: F-actin dismantling through a redox-driven synergy between Mical and cofilin.
Results identify Mical-like as a novel regulator of synaptic growth and FasII endocytic recycling.
Title: MICAL-like Regulates Fasciclin II Membrane Cycling and Synaptic Development.
Mical oxidizes actin stereospecifically to generate actin Met-44-R-sulfoxide.
Title: SelR reverses Mical-mediated oxidation of actin to regulate F-actin dynamics.
actin filament subunits were directly modified by Mical on their conserved pointed-end, critical for filament assembly; Mical posttranslationally oxidized the methionine 44 residue within D-loop of actin, severing filaments and decreasing polymerization
Title: Direct redox regulation of F-actin assembly and disassembly by Mical.
Mical is both necessary and sufficient for semaphorin-plexin-mediated F-actin reorganization in vivo
Title: Mical links semaphorins to F-actin disassembly.
a previously unknown pathway composed of Sox14 and its cytoskeletal target Mical governs dendrite severing during development
Title: A genetic pathway composed of Sox14 and Mical governs severing of dendrites during pruning.
MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion.
Title: MICALs, a family of conserved flavoprotein oxidoreductases, function in plexin-mediated axonal repulsion.
Transgenic expression of interfering RNA molecules demonstrates that MICAL is required in muscles for the higher order arrangement of myofilaments.
Title: Drosophila MICAL regulates myofilament organization and synaptic structure.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables F-actin monooxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables F-actin monooxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables FAD binding	IDA Inferred from Direct Assay more info	PubMed
enables actin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin modification	IDA Inferred from Direct Assay more info	PubMed
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	TAS Traceable Author Statement more info	PubMed
involved_in dendrite morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron remodeling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sarcomere organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synapse assembly involved in innervation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	NAS Non-traceable Author Statement more info	PubMed
colocalizes_with plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: molecule interacting with CasL

Names: CG33208-PA; CG33208-PB; CG33208-PC; CG33208-PD; CG33208-PE; CG33208-PF; CG33208-PG; CG33208-PI; CG33208-PK; CG33208-PL; CG33208-PN; CG33208-PO; CG33208-PP; CG33208-PQ; Mical-PA; Mical-PB; Mical-PC; Mical-PD; Mical-PE; Mical-PF; Mical-PG; Mical-PI; Mical-PK; Mical-PL; Mical-PN; Mical-PO; Mical-PP; Mical-PQ; mical; milchstrasse; molecule that interacts with CasL

NP_001138032.1

EC 1.14.13.-

NP_001247014.1

EC 1.14.13.-

NP_001247015.1

EC 1.14.13.-

NP_001247016.2

EC 1.14.13.-

NP_001303502.1

EC 1.14.13.-

NP_001303503.1

EC 1.14.13.-

NP_788620.1

EC 1.14.13.-

NP_788621.1

EC 1.14.13.-

NP_788622.1

EC 1.14.13.-

NP_788623.1

EC 1.14.13.-

NP_788624.1

EC 1.14.13.-

NP_788625.1

EC 1.14.13.-

NP_788626.2

EC 1.14.13.-

NP_788627.1

EC 1.14.13.-

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

10001554..10042895 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001260086.3 → NP_001247015.1 molecule interacting with CasL, isoform L [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247015.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K6D5

Conserved Domains (5) summary

pfam09528
Location:2081 → 2479

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1097 → 1153

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4592 → 4705

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_001260085.2 → NP_001247014.1 molecule interacting with CasL, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001247014.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K703

Conserved Domains (5) summary

pfam09528
Location:2081 → 2479

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1097 → 1153

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4581 → 4694

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176443.2 → NP_788620.1 molecule interacting with CasL, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788620.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86BA1

Related

FBpp0081686

Conserved Domains (4) summary

cd09439
Location:806 → 862

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:2583 → 2696

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_001316574.1 → NP_001303503.1 molecule interacting with CasL, isoform Q [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A126GUS6

Conserved Domains (4) summary

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:2840 → 2953

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176444.2 → NP_788621.1 molecule interacting with CasL, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788621.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86BA1

Related

FBpp0081685

Conserved Domains (4) summary

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:2851 → 2964

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_001260087.3 → NP_001247016.2 molecule interacting with CasL, isoform N [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K6N6

Conserved Domains (4) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_001316573.1 → NP_001303502.1 molecule interacting with CasL, isoform P [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A0A126GUS4

Conserved Domains (5) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4538 → 4651

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176447.2 → NP_788624.1 molecule interacting with CasL, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788624.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B7Z0U5, F2FB99, Q86BA1, Q86BA2, Q86BA3, Q8MTA1, Q8MUJ8, Q8MUJ9, Q8MUK0, Q9VH41

Related

FBpp0081687

Conserved Domains (5) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4572 → 4685

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176445.2 → NP_788622.1 molecule interacting with CasL, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788622.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B7Z0U5, F2FB99, Q86BA1, Q86BA2, Q86BA3, Q8MTA1, Q8MUJ8, Q8MUJ9, Q8MUK0, Q9VH41

Related

FBpp0081688

Conserved Domains (5) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4572 → 4685

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176449.3 → NP_788626.2 molecule interacting with CasL, isoform O [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86BA1

Conserved Domains (5) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4549 → 4662

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176448.2 → NP_788625.1 molecule interacting with CasL, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788625.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B7Z0U5, F2FB99, Q86BA1, Q86BA2, Q86BA3, Q8MTA1, Q8MUJ8, Q8MUJ9, Q8MUK0, Q9VH41

Related

FBpp0081690

Conserved Domains (5) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4572 → 4685

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176446.2 → NP_788623.1 molecule interacting with CasL, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788623.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B7Z0U5, F2FB99, Q86BA1, Q86BA2, Q86BA3, Q8MTA1, Q8MUJ8, Q8MUJ9, Q8MUK0, Q9VH41

Related

FBpp0081691

Conserved Domains (5) summary

pfam09528
Location:2061 → 2459

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

cd09439
Location:1074 → 1130

LIM_Mical; The LIM domain of Mical (molecule interacting with CasL)

pfam12130
Location:4572 → 4685

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
NM_176450.1 → NP_788627.1 molecule interacting with CasL, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788627.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86BA1

Related

FBpp0081684, FBtr0082206

Conserved Domains (3) summary

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family

cl21454
Location:131 → 255

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins
NM_001144560.3 → NP_001138032.1 molecule interacting with CasL, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001138032.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q86BA1

Conserved Domains (3) summary

cl00030
Location:563 → 629

CH; Calponin homology domain; actin-binding domain which may be present as a single copy or in tandem repeats (which increases binding affinity). The CH domain is found in cytoskeletal and signal transduction proteins, including actin-binding proteins like ...

cl16912
Location:114 → 160

MDR; Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family

cl21454
Location:131 → 255

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A0A0B4K6D5		A0A0B4K6D5
A0A0B4K6N6		A0A0B4K6N6
A0A0B4K703		A0A0B4K703
A0A126GUS4		A0A126GUS4
A0A126GUS6		A0A126GUS6
Q86BA1.1		Q86BA1