GORASP1 golgi reassembly stacking protein 1 [Homo sapiens (human)] - Gene

Summary

Official Symbol: GORASP1provided by HGNC
Official Full Name: golgi reassembly stacking protein 1provided by HGNC
Primary source: HGNC:HGNC:16769
See related: Ensembl:ENSG00000114745 MIM:606867; AllianceGenome:HGNC:16769
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: P65; GOLPH5; GRASP65
Summary: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Expression: Ubiquitous expression in testis (RPKM 12.9), brain (RPKM 12.1) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 3p22.2

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	3	NC_000003.12 (39096599..39107627, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	3	NC_060927.1 (39108763..39119791, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	3	NC_000003.11 (39138090..39149118, complement)

Chromosome 3 - NC_000003.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

GRASP depletion-mediated Golgi fragmentation impairs glycosaminoglycan synthesis, sulfation, and secretion.
Title: GRASP depletion-mediated Golgi fragmentation impairs glycosaminoglycan synthesis, sulfation, and secretion.
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Title: Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Biophysical characterization of intrinsically disordered human Golgi matrix protein GRASP65.
Title: Biophysical characterization of intrinsically disordered human Golgi matrix protein GRASP65.
Markers of malignant prostate cancer cells: Golgi localization of alpha-mannosidase 1A at GM130-GRASP65 site and appearance of high mannose N-glycans on cell surface.
Title: Markers of malignant prostate cancer cells: Golgi localization of α-mannosidase 1A at GM130-GRASP65 site and appearance of high mannose N-glycans on cell surface.
GRASP65 modulates Golgi structure and microtubule organization during cell division.
Title: GRASP65 controls Golgi position and structure during G2/M transition by regulating the stability of microtubules.
DjA1 interacts with GRASP65 to enhance Golgi structure formation through the promotion of GRASP65 trans-oligomerization.
Title: DjA1 maintains Golgi integrity via interaction with GRASP65.
In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells.
Title: Shifted Golgi targeting of glycosyltransferases and α-mannosidase IA from giantin to GM130-GRASP65 results in formation of high mannose N-glycans in aggressive prostate cancer cells.
Results demonstrate a critical role for GRASP55 and GRASP65 in maintaining the stacked structure of the Golgi, which is required for accurate posttranslational modifications in the Golgi. Additionally, the GRASP knockout cell lines developed in this study will be useful tools for studying the role of GRASP proteins in other important cellular processes.
Title: Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.
The authors determined that Golgi membrane ribbon fragmentation increased during the early cytoplasmic phase of cytomegalovirus virion assembly and that Golgi membrane fragmentation in infected cells was dependent on the phosphorylation of an integral cis-Golgi protein, Grasp65.
Title: Phosphorylation of Golgi Peripheral Membrane Protein Grasp65 Is an Integral Step in the Formation of the Human Cytomegalovirus Cytoplasmic Assembly Compartment.
In cells, Mena and actin were required for Golgi ribbon formation after nocodazole washout; in vitro, Mena and microfilaments enhanced GRASP65 oligomerization and Golgi membrane fusion. Thus Mena interacts with GRASP65 to promote local actin polymerization, which facilitates Golgi ribbon linking.
Title: Mena-GRASP65 interaction couples actin polymerization to Golgi ribbon linking.

Submit: New GeneRIF Correction See all GeneRIFs (27)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Variability in the common genetic architecture of social-communication spectrum phenotypes during childhood and adolescence. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Nef	nef	HIV-1 Nef causes the FMS N-glycosylation defect and induces relocalization of the GM130 by activating the p56Hck/MEK/ERK/GRASP65 phosphorylation cascade in the Golgi	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D3S2482 (e-PCR)
- UniSTS:31239

RH12775 (e-PCR)
- UniSTS:55818

G19825 (e-PCR)
- UniSTS:41508

G20726 (e-PCR)
- UniSTS:12581

A001Z36 (e-PCR)
- UniSTS:41509

A006D28 (e-PCR)
- UniSTS:12582

SHGC-76850 (e-PCR)
- UniSTS:24411

SHGC-30755 (e-PCR)
- UniSTS:49251

MARC_16103-16104:1018028206:1 (e-PCR)
- UniSTS:267961

SHGC-30045 (e-PCR)
- UniSTS:40934

GORASP1__6636 (e-PCR)
- UniSTS:463591

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in Golgi organization	NAS Non-traceable Author Statement more info	PubMed
involved_in Golgi ribbon formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein N-linked glycosylation	IEA Inferred from Electronic Annotation more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi membrane	TAS Traceable Author Statement more info
located_in cis-Golgi network	NAS Non-traceable Author Statement more info	PubMed
located_in endoplasmic reticulum-Golgi intermediate compartment membrane	TAS Traceable Author Statement more info

General protein information

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Preferred Names: Golgi reassembly-stacking protein 1

Names: Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; Golgi reassembly and stacking protein 1; golgi reassembly stacking protein 1, 65kDa; golgi reassembly-stacking protein of 65 kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001278789.2 → NP_001265718.1 Golgi reassembly-stacking protein 1 isoform 2

See identical proteins and their annotated locations for NP_001265718.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks two alternate in-frame exons in the 5' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal segment and is shorter than isoform 1.

Source sequence(s)

AI824080, AK001574, AK057043, AK124755, AK303847

Consensus CDS

CCDS63597.1

UniProtKB/TrEMBL

B4E1H8

Related

ENSP00000419123.1, ENST00000479927.6

Conserved Domains (2) summary

cl00117
Location:3 → 110

PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...

cl27544
Location:99 → 203

Herpes_glycop_D; Herpesvirus glycoprotein D/GG/GX domain
NM_001278790.2 → NP_001265719.1 Golgi reassembly-stacking protein 1 isoform 3

See identical proteins and their annotated locations for NP_001265719.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks three alternate exons and uses an alternate splice site in the 5' coding region, compared to variant 1. It encodes isoform 3, which lacks two internal segments and is shorter than isoform 1.

Source sequence(s)

AI824080, AK057043, BC008928

Consensus CDS

CCDS63596.1

UniProtKB/TrEMBL

B3KPY8

Related

ENSP00000395709.2, ENST00000422110.7

Conserved Domains (1) summary

cl00117
Location:3 → 50

PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
NM_001410726.1 → NP_001397655.1 Golgi reassembly-stacking protein 1 isoform 4

Status: REVIEWED

Source sequence(s)

AC138124

Consensus CDS

CCDS93246.1

UniProtKB/TrEMBL

A0A8Q3SHU6

Related

ENSP00000511895.1, ENST00000695414.1
NM_001410731.1 → NP_001397660.1 Golgi reassembly-stacking protein 1 isoform 5

Status: REVIEWED

Source sequence(s)

AC138124

Consensus CDS

CCDS93245.1

UniProtKB/TrEMBL

A0A8Q3WL08

Related

ENSP00000511911.1, ENST00000695430.1
NM_031899.4 → NP_114105.1 Golgi reassembly-stacking protein 1 isoform 1

See identical proteins and their annotated locations for NP_114105.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AI824080, AK001574, AK057043, AK124755

Consensus CDS

CCDS2681.1

UniProtKB/Swiss-Prot

B3KWC8, Q3SYG7, Q8N272, Q96H42, Q9BQQ3

UniProtKB/TrEMBL

A0A8Q3WKF9, C9J9V9

Related

ENSP00000313869.3, ENST00000319283.8

Conserved Domains (3) summary

pfam04495
Location:69 → 205

GRASP55_65; GRASP55/65 PDZ-like domain

cl00117
Location:3 → 100

PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...

cl27544
Location:194 → 298

Herpes_glycop_D; Herpesvirus glycoprotein D/GG/GX domain

RNA

NR_103866.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (4) uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AI824080, AK124755, BC008928, BM470731, DB031221

Related

ENST00000452389.7
NR_103867.2 RNA Sequence

Status: REVIEWED

Description

Transcript Variant: This variant (5) uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AI824080, AK057043, BC064624

Related

ENST00000453680.6

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000003.12 Reference GRCh38.p14 Primary Assembly

Range

39096599..39107627 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047448726.1 → XP_047304682.1 Golgi reassembly-stacking protein 1 isoform X5
XM_006713301.5 → XP_006713364.1 Golgi reassembly-stacking protein 1 isoform X1

UniProtKB/TrEMBL

A0A8Q3WKF9

Related

ENSP00000392993.2, ENST00000437458.4

Conserved Domains (2) summary

pfam04495
Location:74 → 210

GRASP55_65; GRASP55/65 PDZ-like domain

cl00117
Location:26 → 105

PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
XM_011534021.4 → XP_011532323.1 Golgi reassembly-stacking protein 1 isoform X3

UniProtKB/TrEMBL

A0A8Q3WKF9

Conserved Domains (1) summary

cl00117
Location:74 → 150

PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
XM_011534020.4 → XP_011532322.1 Golgi reassembly-stacking protein 1 isoform X2

UniProtKB/TrEMBL

A0A8Q3WKF9

Conserved Domains (2) summary

pfam04495
Location:74 → 180

GRASP55_65; GRASP55/65 PDZ-like domain

cl00117
Location:26 → 105

PDZ; PDZ domain, also called DHR (Dlg homologous region) or GLGF (after a conserved sequence motif). Many PDZ domains bind C-terminal polypeptides, though binding to internal (non-C-terminal) polypeptides and even to lipids has been demonstrated. ...
XM_047448729.1 → XP_047304685.1 Golgi reassembly-stacking protein 1 isoform X8

Related

ENST00000695594.1
XM_047448730.1 → XP_047304686.1 Golgi reassembly-stacking protein 1 isoform X9

Related

ENST00000695416.1
XM_047448728.1 → XP_047304684.1 Golgi reassembly-stacking protein 1 isoform X7
XM_047448725.1 → XP_047304681.1 Golgi reassembly-stacking protein 1 isoform X4
XM_047448727.1 → XP_047304683.1 Golgi reassembly-stacking protein 1 isoform X6