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    Tspan5 tetraspanin 5 [ Mus musculus (house mouse) ]

    Gene ID: 56224, updated on 9-Dec-2024

    Summary

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    Official Symbol
    Tspan5provided by MGI
    Official Full Name
    tetraspanin 5provided by MGI
    Primary source
    MGI:MGI:1928096
    See related
    Ensembl:ENSMUSG00000028152 AllianceGenome:MGI:1928096
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    NET-4; Tm4sf9; 2810455A09Rik; 4930505M03Rik
    Summary
    Enables enzyme binding activity. Involved in protein maturation. Predicted to be located in actin cytoskeleton and nucleoplasm. Predicted to be active in plasma membrane. Is expressed in several structures, including brain; heart; inner ear; limb; and physiological umbilical hernia. Orthologous to human TSPAN5 (tetraspanin 5). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Broad expression in cortex adult (RPKM 21.1), frontal lobe adult (RPKM 20.6) and 23 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Tspan5 in Genome Data Viewer
    Location:
    3 H1; 3 64.42 cM
    Exon count:
    11
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (138447609..138610194)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (138741848..138904433)

    Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_09111 Neighboring gene peptidylprolyl isomerase A pseudogene 3_1437.1 Neighboring gene STARR-seq mESC enhancer starr_09114 Neighboring gene RIKEN cDNA 4930425O10 gene Neighboring gene STARR-seq mESC enhancer starr_09115 Neighboring gene STARR-seq mESC enhancer starr_09116 Neighboring gene CapStarr-seq enhancer MGSCv37_chr3:138406051-138406234 Neighboring gene STARR-seq mESC enhancer starr_09117 Neighboring gene STARR-positive B cell enhancer ABC_E11223 Neighboring gene STARR-positive B cell enhancer mm9_chr3:138527106-138527407 Neighboring gene CapStarr-seq enhancer MGSCv37_chr3:138539885-138540140 Neighboring gene STARR-seq mESC enhancer starr_09124 Neighboring gene predicted gene, 54195 Neighboring gene RAP1, GTP-GDP dissociation stimulator 1 Neighboring gene synaptic nuclear envelope 1 pseudogene Neighboring gene STARR-seq mESC enhancer starr_09125 Neighboring gene STARR-positive B cell enhancer ABC_E6140 Neighboring gene STARR-seq mESC enhancer starr_09128 Neighboring gene predicted gene 4862

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Mouse Tspan-5, a member of the tetraspanin superfamily, is highly expressed in brain cortical structures. García-Frigola C, et al. Neuroreport, 2000 Sep 28. PMID 11043545
    2. Pattern of expression of the tetraspanin Tspan-5 during brain development in the mouse. García-Frigola C, et al. Mech Dev, 2001 Aug. PMID 11472858
    3. Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation. Colbert JD, et al. Proc Natl Acad Sci U S A, 2022 Oct 18. PMID 36215490, Free PMC Article
    4. New insights into the tetraspanin Tspan5 using novel monoclonal antibodies. Saint-Pol J, et al. J Biol Chem, 2017 Jun 9. PMID 28428248, Free PMC Article
    5. Downregulation of Notch modulators, tetraspanin 5 and 10, inhibits osteoclastogenesis in vitro. Zhou J, et al. Calcif Tissue Int, 2014 Sep. PMID 24935633, Free PMC Article

    See all (33) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation.
      Title: Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation.
    2. two TspanC8-specific motifs in the large extracellular domain of Tspan5 that are important for ADAM10 interaction and exit from the endoplasmic reticulum. One of the anti-Tspan5 mAbs does not recognize Tspan5 associated with ADAM10, providing a convenient way to measure the fraction of Tspan5 not associated with ADAM10.
      Title: New insights into the tetraspanin Tspan5 using novel monoclonal antibodies.
    3. Tspan-5 and Tspan-10, are up-regulated during osteoclast differentiation and knockdown of their expression dramatically inhibits osteoclastogenesis
      Title: Downregulation of Notch modulators, tetraspanin 5 and 10, inhibits osteoclastogenesis in vitro.
    4. Up-regulated in pre-osteoclasts during cell differentiation.
      Title: Expression and function of transmembrane-4 superfamily (tetraspanin) proteins in osteoclasts: reciprocal roles of Tspan-5 and NET-6 during osteoclastogenesis.
    5. The level of Tspan-5 expression might be important for distinct phases of neuronal maturation.
      Title: Tetraspanin-5 (Tm4sf9) mRNA expression parallels neuronal maturation in the cerebellum of normal and L7En-2 transgenic mice.
    6. This protein is expressed during brain development from embryonic stages to adulthood.
      Title: Pattern of expression of the tetraspanin Tspan-5 during brain development in the mouse.
    Submit: New GeneRIF Correction

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (2) 
    • Gene trapped (1) 

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables enzyme binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables enzyme binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in positive regulation of Notch signaling pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in positive regulation of Notch signaling pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein localization to plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein localization to plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein maturation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein maturation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of membrane protein ectodomain proteolysis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of membrane protein ectodomain proteolysis ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Component Evidence Code Pubs
    located_in actin cytoskeleton IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in actin cytoskeleton ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  

    General protein information

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    Preferred Names
    tetraspanin-5
    Names
    tetraspan NET-4
    tetraspanin Tspan-5
    transmembrane 4 superfamily member 9

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001428885.1 → NP_001415814.1  tetraspanin-5 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC110574, AC114621
      UniProtKB/TrEMBL
      D3Z641

    2. NM_019571.6 → NP_062517.1  tetraspanin-5 isoform 1

      See identical proteins and their annotated locations for NP_062517.1

      Status: VALIDATED

      Source sequence(s)
      AC110574, AC114621
      Consensus CDS
      CCDS17870.1
      UniProtKB/Swiss-Prot
      P62080
      UniProtKB/TrEMBL
      Q9CYT0
      Related
      ENSMUSP00000029800.3, ENSMUST00000029800.9
      Conserved Domains (2) summary
      cd03159
      Location:113 → 233
      TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...
      pfam00335
      Location:15 → 257
      Tetraspannin; Tetraspanin family

    RNA

    1. NR_121195.2 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AC110574, AC114621
      Related
      ENSMUST00000142890.8

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000069.7 Reference GRCm39 C57BL/6J

      Range
      138447609..138610194
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_030252697.1 → XP_030108557.1  tetraspanin-5 isoform X2

      Related
      ENSMUSP00000113230.2, ENSMUST00000119993.8
      Conserved Domains (1) summary
      cd03159
      Location:42 → 162
      TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...

    2. XM_006501737.5 → XP_006501800.1  tetraspanin-5 isoform X2

      See identical proteins and their annotated locations for XP_006501800.1

      UniProtKB/TrEMBL
      D3Z641
      Conserved Domains (1) summary
      cd03159
      Location:42 → 162
      TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...

    3. XM_006501736.5 → XP_006501799.1  tetraspanin-5 isoform X1

      UniProtKB/TrEMBL
      Q9CYT0
      Conserved Domains (1) summary
      cd03159
      Location:156 → 276
      TM4SF9_like_LEL; Tetraspanin, extracellular domain or large extracellular loop (LEL), TM4SF9_like subfamily. Tetraspanins are trans-membrane proteins with 4 trans-membrane segments. Both the N- and C-termini lie on the intracellular side of the membrane. This alignment ...

    RNA

    1. XR_375551.5 RNA Sequence

    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P62080.1 GenPept UniProtKB/Swiss-Prot:P62080

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