Gls2 glutaminase 2 (liver, mitochondrial) [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gls2provided by MGI
Official Full Name: glutaminase 2 (liver, mitochondrial)provided by MGI
Primary source: MGI:MGI:2143539
See related: Ensembl:ENSMUSG00000044005 AllianceGenome:MGI:2143539
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: GA; GLS; Lga; A330074B06Rik
Summary: Predicted to enable glutaminase activity. Predicted to be involved in glutamate biosynthetic process and glutamine catabolic process. Predicted to act upstream of or within reactive oxygen species metabolic process and regulation of apoptotic process. Located in mitochondrion. Is expressed in several structures, including alimentary system; limb; nervous system; skeleton; and urinary system. Orthologous to human GLS2 (glutaminase 2). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in liver adult (RPKM 304.4) and liver E18 (RPKM 21.8) See more
Orthologs: human all
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Genomic context

Location:: 10 D3; 10 76.44 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (128030504..128045873)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (128194635..128210004)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LRH-1 activation alleviates diabetes-induced podocyte injury by promoting GLS2-mediated glutaminolysis.
Title: LRH-1 activation alleviates diabetes-induced podocyte injury by promoting GLS2-mediated glutaminolysis.
Role of Glutaminase 2 in Promoting CD4+ T Cell Production of Interleukin-2 by Supporting Antioxidant Defense in Systemic Lupus Erythematosus.
Title: Role of Glutaminase 2 in Promoting CD4+ T Cell Production of Interleukin-2 by Supporting Antioxidant Defense in Systemic Lupus Erythematosus.
Glutaminase 2 knockdown reduces hyperammonemia and associated lethality of urea cycle disorder mouse model.
Title: Glutaminase 2 knockdown reduces hyperammonemia and associated lethality of urea cycle disorder mouse model.
Data (including data from studies in knockout/transgenic mice) suggest Ppp2ca supports cortical neuronal growth and cognitive function via regulating p73/Gls2 signal transduction in neurons of hippocampus. Ppp2ca gene knock-out results in embryonic cortical atrophy with learning/memory deficits. (Ppp2ca = protein phosphatase 2a catalytic subunit alpha isoform; p73 = transformation related protein 73; Gls2 = glutaminase-2)
Title: Protein phosphatase 2ACα gene knock-out results in cortical atrophy through activating hippo cascade in neuronal progenitor cells.
Gls2 expression is regulated by the dietary protein/carbohydrate ratio. This effect was not observed in Ppara-null mice.
Title: PPARα Downregulates Hepatic Glutaminase Expression in Mice Fed Diets with Different Protein:Carbohydrate Ratios.
Study demonstrated expression of alternative transcripts of the mammalian Gls2 gene. Transcriptional mechanisms giving rise to GLS2 variants and isolation of novel GLS2 transcripts in human, rat and mouse are presented.
Title: Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.
Phosphate-activated glutaminase (GLS2) is a p53-inducible regulator of glutamine metabolism and reactive oxygen species
Title: Phosphate-activated glutaminase (GLS2), a p53-inducible regulator of glutamine metabolism and reactive oxygen species.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH124744 (e-PCR)
- UniSTS:161566

AA408693 (e-PCR)
- UniSTS:160042

AI195532 (e-PCR)
- UniSTS:160043

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

Gls2 (e-PCR), detects polymorphism
- UniSTS:545939

RH125813 (e-PCR)
- UniSTS:162559

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables glutaminase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutaminase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glutamate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutamine metabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of apoptotic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutaminase liver isoform, mitochondrial

Names: L-glutaminase; L-glutamine amidohydrolase; liver mitochondrial glutaminase

NP_001028436.2

EC 3.5.1.2

NP_001272706.1

EC 3.5.1.2

NP_001272708.1

EC 3.5.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001033264.3 → NP_001028436.2 glutaminase liver isoform, mitochondrial isoform 1 precursor

See identical proteins and their annotated locations for NP_001028436.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC135859, AK220231

Consensus CDS

CCDS24263.1

UniProtKB/Swiss-Prot

B2RUF2, Q3TMJ6, Q571F8

Related

ENSMUSP00000047376.7, ENSMUST00000044776.13

Conserved Domains (4) summary

sd00045
Location:490 → 516

ANK; ANK repeat [structural motif]

pfam12796
Location:490 → 572

Ank_2; Ankyrin repeats (3 copies)

pfam17959
Location:72 → 155

EF-hand_14; EF-hand domain

pfam04960
Location:177 → 463

Glutaminase
NM_001285777.1 → NP_001272706.1 glutaminase liver isoform, mitochondrial isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AC135859

Conserved Domains (4) summary

cd00204
Location:301 → 389

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:17 → 274

Glutaminase; Glutaminase

pfam13637
Location:331 → 383

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:301 → 327

ANK; ANK repeat [structural motif]
NM_001285779.1 → NP_001272708.1 glutaminase liver isoform, mitochondrial isoform 3

See identical proteins and their annotated locations for NP_001272708.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' region and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1.

Source sequence(s)

AC135859

UniProtKB/TrEMBL

Q3TGF1

Conserved Domains (4) summary

cd00204
Location:225 → 313

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:1 → 198

Glutaminase; Glutaminase

pfam13637
Location:255 → 307

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:225 → 251

ANK; ANK repeat [structural motif]