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    Usp8 ubiquitin specific peptidase 8 [ Mus musculus (house mouse) ]

    Gene ID: 84092, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Usp8provided by MGI
    Official Full Name
    ubiquitin specific peptidase 8provided by MGI
    Primary source
    MGI:MGI:1934029
    See related
    Ensembl:ENSMUSG00000027363 AllianceGenome:MGI:1934029
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    Ubpy; mKIAA0055
    Summary
    Enables cysteine-type deubiquitinase activity. Involved in endosome organization and protein deubiquitination. Acts upstream of or within Ras protein signal transduction. Located in cytoplasmic vesicle membrane; cytosol; and midbody. Is expressed in several structures, including brain; genitourinary system; gut; hemolymphoid system gland; and sensory organ. Human ortholog(s) of this gene implicated in ACTH-secreting pituitary adenoma. Orthologous to human USP8 (ubiquitin specific peptidase 8). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in testis adult (RPKM 45.2), bladder adult (RPKM 14.4) and 25 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Usp8 in Genome Data Viewer
    Location:
    2 F1; 2 61.76 cM
    Exon count:
    19
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (126549280..126601234)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (126707201..126759314)

    Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene GA repeat binding protein, beta 1 Neighboring gene zinc finger, BED domain containing 4 pseudogene Neighboring gene RIKEN cDNA A630026N12 gene Neighboring gene STARR-seq mESC enhancer starr_05607 Neighboring gene STARR-seq mESC enhancer starr_05610 Neighboring gene polymerase (RNA) II (DNA directed) polypeptide L pseudogene Neighboring gene ubiquitin specific peptidase 50 Neighboring gene transient receptor potential cation channel, subfamily M, member 7 Neighboring gene STARR-positive B cell enhancer ABC_E11158

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages. Joshi K, et al. Infect Immun, 2024 Feb 13. PMID 38174929, Free PMC Article
    2. The ESCRT-deubiquitinating enzyme USP8 in the cervical spinal cord of wild-type and Vps54-recessive (wobbler) mutant mice. Paiardi C, et al. Histochem Cell Biol, 2014 Jan. PMID 23615794
    3. Expression and localization of the deubiquitinating enzyme mUBPy in wobbler mouse testis during spermiogenesis. Chianese R, et al. Gen Comp Endocrinol, 2010 Apr 1. PMID 19800341
    4. Regulation of ubiquitin-specific processing protease 8 suppresses neuroinflammation. Zhu L, et al. Mol Cell Neurosci, 2015 Jan. PMID 24861766
    5. Expression of the deubiquitinating enzyme mUBPy in the mouse brain. Bruzzone F, et al. Brain Res, 2008 Feb 21. PMID 18206859

    See all (62) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.
      Title: USP8-governed GPX4 homeostasis orchestrates ferroptosis and cancer immunotherapy.
    2. Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages.
      Title: Brucella targets the host ubiquitin-specific protease, Usp8, through the effector protein, TcpB, for facilitating infection of macrophages.
    3. Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.
      Title: Inhibition of ubiquitin specific peptidase 8 is effective against 5-fluorouracil resistance in colon cancer via suppressing EGFR and EGFR-mediated signaling pathways.
    4. Central role of ubiquitin-specific protease 8 in leptin signaling pathway in pulmonary arterial hypertension.
      Title: Central role of ubiquitin-specific protease 8 in leptin signaling pathway in pulmonary arterial hypertension.
    5. USP8 mitigates cognitive dysfunction of mice with sepsis-associated encephalopathy.
      Title: USP8 mitigates cognitive dysfunction of mice with sepsis-associated encephalopathy.
    6. Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious Osteomyelitis by Targeting RIPK2 Ubiquitination.
      Title: Ubiquitin-Specific Peptidase 8 Is Critical for the Onset of Infectious Osteomyelitis by Targeting RIPK2 Ubiquitination.
    7. Ubiquitinspecific protease 8 ameliorates lipopolysaccharideinduced spleen injury via suppression of NFkappaB and MAPK signaling pathways.
      Title: Ubiquitin‑specific protease 8 ameliorates lipopolysaccharide‑induced spleen injury via suppression of NF‑κB and MAPK signaling pathways.
    8. USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.
      Title: USP8 inhibition reshapes an inflamed tumor microenvironment that potentiates the immunotherapy.
    9. Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
      Title: Deubiquitinating Enzyme USP8 Is Essential for Skeletogenesis by Regulating Wnt Signaling.
    10. Deubiquitination of EPG5 by USP8 guards the autophagic flux in embryonic stem cells (ESCs) to maintain their stemness. This work uncovers a novel crosstalk pathway between ubiquitination and autophagy through USP8-EPG5 interaction to regulate the stemness of ESCs.
      Title: USP8 maintains embryonic stem cell stemness via deubiquitination of EPG5.
    Submit: New GeneRIF Correction See all GeneRIFs (27)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (3) 
    • Gene trapped (1) 
    • Targeted (2)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables K48-linked deubiquitinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables K63-linked deubiquitinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables SH3 domain binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables cysteine-type deubiquitinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cysteine-type deubiquitinase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cysteine-type deubiquitinase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in Ras protein signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within Ras protein signal transduction IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    involved_in cellular response to nerve growth factor stimulus ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in endosome organization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in endosome organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within mitotic cytokinesis ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in negative regulation of lysosomal protein catabolic process ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of amyloid fibril formation ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in positive regulation of canonical Wnt signaling pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K48-linked deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein K63-linked deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    acts_upstream_of_or_within protein deubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein deubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein deubiquitination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in protein deubiquitination ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of protein catabolic process at postsynapse, modulating synaptic transmission ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein localization ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein localization ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in regulation of protein stability ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in regulation of protein stability ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    Component Evidence Code Pubs
    located_in acrosomal membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in cytosol ISO
    Inferred from Sequence Orthology
    more info
    		  
    is_active_in dendritic spine IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in dendritic spine ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in early endosome ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in endosome membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in glutamatergic synapse ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in intracellular anatomical structure IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in midbody IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in midbody IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in midbody ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in postsynaptic density IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in postsynaptic density ISO
    Inferred from Sequence Orthology
    more info
    		  
    located_in postsynaptic density ISO
    Inferred from Sequence Orthology
    more info
    		  

    General protein information

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    Preferred Names
    ubiquitin carboxyl-terminal hydrolase 8
    Names
    deubiquitinating enzyme 8
    putative deubiquitinating enzyme
    ubiquitin isopeptidase Y
    ubiquitin thioesterase 8
    ubiquitin thiolesterase 8
    ubiquitin-specific-processing protease 8
    NP_001239509.1
    NP_001409160.1
    NP_001409161.1
    NP_001409162.1
    NP_001409163.1
    NP_062703.2

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001252580.2 → NP_001239509.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
      Source sequence(s)
      AL732330
      Consensus CDS
      CCDS57185.1
      UniProtKB/TrEMBL
      A2AI52, Q3UZ62
      Related
      ENSMUSP00000106046.3, ENSMUST00000110416.3
      Conserved Domains (4) summary
      smart00450
      Location:210 → 321
      RHOD; Rhodanese Homology Domain
      cd02674
      Location:751 → 1080
      Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:749 → 1079
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam08969
      Location:6 → 126
      USP8_dimer; USP8 dimerization domain

    2. NM_001422231.1 → NP_001409160.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AL732330

    3. NM_001422232.1 → NP_001409161.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AL732330

    4. NM_001422233.1 → NP_001409162.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 5

      Status: VALIDATED

      Source sequence(s)
      AL732330

    5. NM_001422234.1 → NP_001409163.1  ubiquitin carboxyl-terminal hydrolase 8 isoform 6

      Status: VALIDATED

      Source sequence(s)
      AL732330

    6. NM_019729.4 → NP_062703.2  ubiquitin carboxyl-terminal hydrolase 8 isoform 2

      See identical proteins and their annotated locations for NP_062703.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
      Source sequence(s)
      AL732330
      Consensus CDS
      CCDS16687.1
      UniProtKB/Swiss-Prot
      A2AI53, Q80U87, Q80YP2, Q8R0D3, Q9EQU1, Q9WVP5
      UniProtKB/TrEMBL
      Q3UZ62
      Related
      ENSMUSP00000028841.8, ENSMUST00000028841.14
      Conserved Domains (4) summary
      smart00450
      Location:199 → 310
      RHOD; Rhodanese Homology Domain
      cd02674
      Location:740 → 1069
      Peptidase_C19R; A subfamily of peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
      pfam00443
      Location:738 → 1068
      UCH; Ubiquitin carboxyl-terminal hydrolase
      pfam08969
      Location:6 → 115
      USP8_dimer; USP8 dimerization domain

    RNA

    1. NR_185498.1 RNA Sequence

      Status: VALIDATED

      Source sequence(s)
      AL732330

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000068.8 Reference GRCm39 C57BL/6J

      Range
      126549280..126601234
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q80U87.2 GenPept UniProtKB/Swiss-Prot:Q80U87

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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