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    Mcf2l MCF.2 cell line derived transforming sequence-like [ Rattus norvegicus (Norway rat) ]

    Gene ID: 117020, updated on 9-Dec-2024

    Summary

    Symbol
    Mcf2lprovided by RGD
    Full Name
    MCF.2 cell line derived transforming sequence-likeprovided by RGD
    Primary source
    RGD:619782
    See related
    EnsemblRapid:ENSRNOG00000028426 AllianceGenome:RGD:619782
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    Ost; mcf 2l
    Summary
    Enables guanyl-nucleotide exchange factor activity. Involved in intracellular signal transduction. Located in cytoplasm. Orthologous to human MCF2L (MCF.2 cell line derived transforming sequence like). [provided by Alliance of Genome Resources, Dec 2024]
    Expression
    Biased expression in Brain (RPKM 86.9), Kidney (RPKM 46.2) and 8 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See Mcf2l in Genome Data Viewer
    Location:
    16q12.5
    Exon count:
    38
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 16 NC_086034.1 (83209277..83355683, complement)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 16 NC_051351.1 (76507133..76652893, complement)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 16 NC_005115.4 (81839686..81986007, complement)

    Chromosome 16 - NC_086034.1Genomic Context describing neighboring genes Neighboring gene small ribosomal subunit protein eS19-like Neighboring gene coagulation factor X Neighboring gene uncharacterized LOC134482421 Neighboring gene coagulation factor VII Neighboring gene ATPase phospholipid transporting 11A Neighboring gene uncharacterized LOC102548949 Neighboring gene uncharacterized LOC134482533

    Genomic regions, transcripts, and products

    Expression

    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    General gene information

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables 1-phosphatidylinositol binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables guanyl-nucleotide exchange factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables guanyl-nucleotide exchange factor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables guanyl-nucleotide exchange factor activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables guanyl-nucleotide exchange factor activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables phosphatidylinositol binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables phosphatidylinositol binding ISO
    Inferred from Sequence Orthology
    more info
     
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
     
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
     
    located_in endomembrane system ISO
    Inferred from Sequence Orthology
    more info
     
    located_in extrinsic component of cytoplasmic side of plasma membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in lamellipodium ISO
    Inferred from Sequence Orthology
    more info
     
    located_in membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    guanine nucleotide exchange factor DBS
    Names
    DBL's big sister
    MCF2-transforming sequence-like protein
    OST oncogene
    mcf.2 transforming sequence-like

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001413769.1NP_001400698.1  guanine nucleotide exchange factor DBS isoform 2

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000016
      UniProtKB/TrEMBL
      D4A7F3
      Related
      ENSRNOP00000083374.2, ENSRNOT00000100402.2
    2. NM_053951.2NP_446403.2  guanine nucleotide exchange factor DBS isoform 1

      See identical proteins and their annotated locations for NP_446403.2

      Status: VALIDATED

      Source sequence(s)
      CH473970
      UniProtKB/TrEMBL
      A6IWK6, D4A7F3
      Conserved Domains (6) summary
      cd11857
      Location:10821134
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:46191
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:603780
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:322524
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:787918
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:801915
      PH; PH domain

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086034.1 Reference GRCr8

      Range
      83209277..83355683 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_039094165.2XP_038950093.1  guanine nucleotide exchange factor DBS isoform X8

      UniProtKB/TrEMBL
      A0A8I5Y6G8
      Related
      ENSRNOP00000077897.2, ENSRNOT00000101752.2
      Conserved Domains (5) summary
      cd11857
      Location:11151167
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:76221
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:633810
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:352554
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:817948
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    2. XM_039094166.2XP_038950094.1  guanine nucleotide exchange factor DBS isoform X12

      UniProtKB/Swiss-Prot
      Q63406
      UniProtKB/TrEMBL
      D4A7F3
      Related
      ENSRNOP00000068993.2, ENSRNOT00000079096.3
      Conserved Domains (5) summary
      cd11857
      Location:10591111
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:76221
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:633810
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:352554
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:817948
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    3. XM_063275044.1XP_063131114.1  guanine nucleotide exchange factor DBS isoform X16

      UniProtKB/TrEMBL
      D4A7F3
    4. XM_017599979.3XP_017455468.1  guanine nucleotide exchange factor DBS isoform X11

      UniProtKB/TrEMBL
      A0A8I5Y6G8
    5. XM_006253421.5XP_006253483.1  guanine nucleotide exchange factor DBS isoform X6

      UniProtKB/TrEMBL
      A0A8I5ZZZ7, D4A7F3
      Conserved Domains (6) summary
      cd11857
      Location:11281180
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:145290
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:702879
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:421623
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:8861017
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:9001014
      PH; PH domain
    6. XM_039094163.2XP_038950091.1  guanine nucleotide exchange factor DBS isoform X7

      UniProtKB/TrEMBL
      D4A7F3
      Conserved Domains (5) summary
      cd11857
      Location:11281180
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:145290
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:702879
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:421623
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:8861017
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    7. XM_039094162.2XP_038950090.1  guanine nucleotide exchange factor DBS isoform X4

      UniProtKB/TrEMBL
      A0A8I5Y6G8, A0A8I5ZWU6
      Conserved Domains (5) summary
      cd11857
      Location:11531205
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:145290
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:702879
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:421623
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:8861017
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    8. XM_039094160.2XP_038950088.1  guanine nucleotide exchange factor DBS isoform X3

      UniProtKB/TrEMBL
      A0A8I5Y6G8
      Conserved Domains (5) summary
      cd11857
      Location:11811233
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:145290
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:702879
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:421623
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:8861017
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    9. XM_006253416.5XP_006253478.1  guanine nucleotide exchange factor DBS isoform X1

      UniProtKB/TrEMBL
      A0A8I5Y6G8
      Related
      ENSRNOP00000085354.2, ENSRNOT00000111168.2
      Conserved Domains (6) summary
      cd11857
      Location:11841236
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:145290
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:702879
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:421623
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:8861017
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:9001014
      PH; PH domain
    10. XM_039094159.2XP_038950087.1  guanine nucleotide exchange factor DBS isoform X2

      UniProtKB/TrEMBL
      A0A8I5Y6G8
      Conserved Domains (5) summary
      cd11857
      Location:11841236
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:145290
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:702879
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:421623
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:8861017
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    11. XM_006253423.5XP_006253485.1  guanine nucleotide exchange factor DBS isoform X9

      UniProtKB/TrEMBL
      A0A8I5Y6G8
      Conserved Domains (6) summary
      cd11857
      Location:10891141
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:50195
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:607784
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:326528
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:791922
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:805919
      PH; PH domain
    12. XM_039094167.2XP_038950095.1  guanine nucleotide exchange factor DBS isoform X13

      UniProtKB/TrEMBL
      A0A8L2QMZ3, D4A7F3
      Related
      ENSRNOP00000047024.6, ENSRNOT00000043170.8
      Conserved Domains (5) summary
      cd11857
      Location:10331085
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:50195
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:607784
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:326528
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:791922
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
    13. XM_063275040.1XP_063131110.1  guanine nucleotide exchange factor DBS isoform X5

      UniProtKB/TrEMBL
      A0A8I5Y6G8
    14. XM_006253424.5XP_006253486.1  guanine nucleotide exchange factor DBS isoform X10

      UniProtKB/TrEMBL
      A0A0G2KA20, A0A8I5Y6G8
      Related
      ENSRNOP00000075224.2, ENSRNOT00000087095.3
      Conserved Domains (6) summary
      cd11857
      Location:10831135
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      smart00516
      Location:44189
      SEC14; Domain in homologues of a S. cerevisiae phosphatidylinositol transfer protein (Sec14p)
      cd00160
      Location:601778
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:320522
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:785916
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      pfam00169
      Location:799913
      PH; PH domain
    15. XM_063275042.1XP_063131112.1  guanine nucleotide exchange factor DBS isoform X15

      UniProtKB/TrEMBL
      D4A7F3
    16. XM_063275041.1XP_063131111.1  guanine nucleotide exchange factor DBS isoform X14

      UniProtKB/TrEMBL
      A0A8I5Y6G8
    17. XM_039094169.2XP_038950097.1  guanine nucleotide exchange factor DBS isoform X17

      UniProtKB/TrEMBL
      A0A8I5Y6G8
      Conserved Domains (5) summary
      cd11857
      Location:926978
      SH3_DBS; Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
      cd00160
      Location:444621
      RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
      cd00176
      Location:163365
      SPEC; Spectrin repeats, found in several proteins involved in cytoskeletal structure; family members include spectrin, alpha-actinin and dystrophin; the spectrin repeat forms a three helix bundle with the second helix interrupted by proline in some sequences; ...
      cd01227
      Location:628759
      PH_Dbs; DBL's big sister protein pleckstrin homology (PH) domain
      cl15787
      Location:535
      SEC14; Sec14p-like lipid-binding domain. Found in secretory proteins, such as S. cerevisiae phosphatidylinositol transfer protein (Sec14p), and in lipid regulated proteins such as RhoGAPs, RhoGEFs and neurofibromin (NF1). SEC14 domain of Dbl is known to ...