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    ctp1 CtIP family endonuclease [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2538706, updated on 18-Sep-2024

    Summary

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    Official Symbol
    ctp1
    Official Full Name
    CtIP family endonuclease
    Primary source
    PomBase:SPCC338.08
    Locus tag
    SPOM_SPCC338.08
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    mug38; nip1; slr9
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    Genomic context

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    See ctp1 in Genome Data Viewer
    Location:
    chromosome: III
    Exon count:
    2
    Sequence:
    Chromosome: III; NC_003421.2 (1358737..1360232, complement)

    Chromosome III - NC_003421.2Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene methyltransferase Rrg1 Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene cytochrome c oxidase subunit V Neighboring gene ncRNA Neighboring gene tRNA-Thr

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_003421.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Ctp1 protein-DNA filaments promote DNA bridging and DNA double-strand break repair. Andres SN, et al. J Biol Chem, 2019 Mar 1. PMID 30626735, Free PMC Article
    2. Two separable functions of Ctp1 in the early steps of meiotic DNA double-strand break repair. Ma L, et al. Nucleic Acids Res, 2015 Sep 3. PMID 26130711, Free PMC Article
    3. Phosphorylation-regulated binding of Ctp1 to Nbs1 is critical for repair of DNA double-strand breaks. Dodson GE, et al. Cell Cycle, 2010 Apr 15. PMID 20421724, Free PMC Article
    4. Molecular characterization of the role of the Schizosaccharomyces pombe nip1+/ctp1+ gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex. Akamatsu Y, et al. Mol Cell Biol, 2008 Jun. PMID 18378696, Free PMC Article
    5. Ctp1 and the MRN-complex are required for endonucleolytic Rec12 removal with release of a single class of oligonucleotides in fission yeast. Rothenberg M, et al. PLoS Genet, 2009 Nov. PMID 19911044, Free PMC Article

    See all (40) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity.
      Title: A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity.
    2. an important role for Ctp1-regulated DNA strand coordination required for DNA DSB repair in S. pombe
      Title: Ctp1 protein-DNA filaments promote DNA bridging and DNA double-strand break repair.
    3. CtIP/Ctp1/Sae2/Com1 role in removal of DNA double strand breaks through DSB repair by homologous recombination is reviewed.
      Title: CtIP/Ctp1/Sae2, molecular form fit for function.
    4. a mre11 mutant defective in endonucleolytic activity, the same mutant lacking Ctp1, or the triple mutant also lacking the putative hairpin nuclease Pso2 showed wild-type levels of repair. Thus, MRN (Mre11-Rad50-Nbs1)may act to recruit the hairpin opening activity that allows subsequent repair
      Title: Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe.
    5. The authors concludes that the intracellular Ctp1 C-terminal portion is essential for clipping, while the N-terminal portion is sufficient for DNA double-strand break end-resection.
      Title: Two separable functions of Ctp1 in the early steps of meiotic DNA double-strand break repair.
    6. Tetrameric Ctp1 coordinates DNA binding and DNA bridging in DNA double-strand-break repair.
      Title: Tetrameric Ctp1 coordinates DNA binding and DNA bridging in DNA double-strand-break repair.
    7. these data propose that release of the MRN complex and Ku from DNA ends by Mre11 nuclease activity and Ctp1 is a critical step required to expose ssDNA for replication protein A localization and ensuing homologous recombination repair.
      Title: Release of Ku and MRN from DNA ends by Mre11 nuclease activity and Ctp1 is required for homologous recombination repair of double-strand breaks.
    8. In fission yeast, found only one class of oligonucleotides bound to Rec12 ranging in length from 17 to 27 nucleotides. Ctp1, Rad50, and the nuclease activity of Rad32, the fission yeast homolog of Mre11, are required for endonucleolytic Rec12 removal.
      Title: Ctp1 and the MRN-complex are required for endonucleolytic Rec12 removal with release of a single class of oligonucleotides in fission yeast.
    9. Rad32 nuclease has the catalytic site for Rec12-oligonucleotide generation and is activated by Ctp1, which plays an additional role in meiotic recombination.
      Title: Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.
    10. Tethering of Ctp1 to a flexible Nbs1 arm suggests a mechanism for restricting DNA end processing and homologous recombination activities of Sae2/Ctp1/CtIP to the immediate vicinity of double-strand breaks.
      Title: Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.
    Submit: New GeneRIF Correction See all GeneRIFs (15)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables DNA end binding EXP
    Inferred from Experiment
    more info
    		 PubMed 
    enables DNA-DNA tethering activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables Y-form DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables bubble DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables double-strand/single-strand DNA junction binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables double-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT enables double-stranded DNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables endodeoxyribonuclease activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables flap-structured DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables single-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA double-strand break processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in double-strand break repair involved in meiotic recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in double-strand break repair via homologous recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in double-strand break repair via homologous recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in double-strand break repair via nonhomologous end joining IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in double-strand break repair via nonhomologous end joining IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in gene conversion at mating-type locus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiotic DNA double-strand break processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic DNA replication checkpoint signaling IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitotic recombination-dependent replication fork processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in replication fork processing IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in stalled replication fork localization to nuclear periphery IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in site of double-strand break IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    CtIP family endonuclease

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003421.2 Reference assembly

      Range
      1358737..1360232 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001023148.4 → NP_588159.2  CtIP family endonuclease [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_588159.2

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      A9LJ21, O74986
      Conserved Domains (1) summary
      pfam08573
      Location:210 → 292
      SAE2; DNA repair protein endonuclease SAE2/CtIP C-terminus
    Nucleotide Protein
    Heading Accession and Version

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