KIP3 tubulin-dependent ATPase KIP3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: KIP3
Official Full Name: tubulin-dependent ATPase KIP3
Primary source: SGD:S000003184
Locus tag: YGL216W
See related: AllianceGenome:SGD:S000003184; FungiDB:YGL216W; VEuPathDB:YGL216W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables plus-end-directed microtubule motor activity and tubulin-dependent ATPase activity. Involved in several processes, including meiotic sister chromatid segregation; microtubule cytoskeleton organization; and regulation of organelle organization. Acts upstream of or within microtubule nucleation. Located in cytoplasmic microtubule and nuclear microtubule. Orthologous to several human genes including KIF18A (kinesin family member 18A). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See KIP3 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (84885..87302)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Random Sideward Steps of the Kinesin-8 Kip3
Title: Three-Dimensional Optical Tweezers Tracking Resolves Random Sideward Steps of the Kinesin-8 Kip3.
The single kinesin-8 motor Kip3 alternately mediates both catastrophe and rescue of the bud microtubule (MT). Kip3 accumulates in a length-dependent manner along the lattice of MTs within the bud, yet induces catastrophe spatially near the bud tip.
Title: Spatial control of microtubule length and lifetime by opposing stabilizing and destabilizing functions of Kinesin-8.
Kip3 directly suppresses spindle microtubule polymerization, limiting midzone length.
Title: The kinesin-8 Kip3 scales anaphase spindle length by suppression of midzone microtubule polymerization.
the motion of yeast (Kip3) and human (Kif18A) kinesin-8s
Title: Kinesin-8 is a low-force motor protein with a weakly bound slip state.
antiparallel microtubule-sliding activity of the budding yeast kinesin-8, Kip3
Title: Microtubule-sliding activity of a kinesin-8 promotes spindle assembly and spindle-length control.
Bud6p and Kip3p play antagonistic roles in controlling the length of microtubules contacting the bud.
Title: Mechanism for astral microtubule capture by cortical Bud6p priming spindle polarity in S. cerevisiae.
Studies indicate that the motor protein Kip3 (in the kinesin-8 family) is an ideal model system to reconstitute high-density traffic on microtubules.
Title: Molecular crowding creates traffic jams of kinesin motors on microtubules.
A model in which TORC1 and Clb5/CDK act coordinately to promote both spindle assembly via a pathway involving Kip3 and S phase progression.
Title: TORC1 kinase and the S-phase cyclin Clb5 collaborate to promote mitotic spindle assembly and DNA replication in S. cerevisiae.
Study found that after binding at a random position on a microtubule and walking to the plus end, an individual Kip3p molecule pauses there until an incoming Kip3p molecule bumps it off.
Title: Kinesin-8 motors act cooperatively to mediate length-dependent microtubule depolymerization.
characterization of frictional drag force of KIP3(kinesin-8 motor proteins) interacting with microtubule tracks; argue these frictional forces arise from breaking bonds between motor domains & the microtubule & they limit speed & efficiency of kinesin
Title: Protein friction limits diffusive and directed movements of kinesin motors on microtubules.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables plus-end-directed microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables plus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables tubulin-dependent ATPase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule depolymerization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within microtubule nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic sister chromatid segregation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of microtubule polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration along microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in plus-end specific microtubule depolymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of mitotic spindle elongation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of kinesin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of kinesin complex	TAS Traceable Author Statement more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
is_active_in mitotic spindle astral microtubule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in mitotic spindle midzone	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: tubulin-dependent ATPase KIP3

NP_011299.1

Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

84885..87302

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181081.1 → NP_011299.1 TPA: tubulin-dependent ATPase KIP3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011299.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTU0, P53086

UniProtKB/TrEMBL

G2WDP3

Conserved Domains (1) summary

cd01370
Location:82 → 438

KISc_KIP3_like; Kinesin motor domain, KIP3-like subgroup