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    CUE1 Cue1p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855306, updated on 9-Dec-2024

    Summary

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    Official Symbol
    CUE1
    Official Full Name
    Cue1p
    Primary source
    SGD:S000004877
    Locus tag
    YMR264W
    See related
    AllianceGenome:SGD:S000004877; FungiDB:YMR264W; VEuPathDB:YMR264W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    KIS4
    Summary
    Enables ubiquitin binding activity and ubiquitin-protein transferase activator activity. Involved in ERAD pathway and establishment of protein localization to endoplasmic reticulum membrane. Located in endoplasmic reticulum membrane. Part of Doa10p ubiquitin ligase complex and Hrd1p ubiquitin ligase ERAD-L complex. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See CUE1 in Genome Data Viewer
    Location:
    chromosome: XIII
    Exon count:
    1
    Sequence:
    Chromosome: XIII; NC_001145.3 (795805..796416)

    Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene putative endodeoxyribonuclease Neighboring gene Sap30p Neighboring gene uncharacterized protein Neighboring gene Rsn1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001145.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae. Buchanan BW, et al. J Vis Exp, 2016 Apr 18. PMID 27167179, Free PMC Article
    2. Determinants of RING-E2 fidelity for Hrd1p, a membrane-anchored ubiquitin ligase. Bazirgan OA, et al. J Biol Chem, 2006 Dec 22. PMID 17035235
    3. Autoregulation of an E2 enzyme by ubiquitin-chain assembly on its catalytic residue. Ravid T, et al. Nat Cell Biol, 2007 Apr. PMID 17310239
    4. A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms. Metzger MB, et al. Mol Cell, 2013 May 23. PMID 23665230, Free PMC Article
    5. A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation. Kostova Z, et al. J Cell Sci, 2009 May 1. PMID 19366730, Free PMC Article

    See all (85) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Cue1 positions Ubc7 and the distal acceptor ubiquitin for rapid polyubiquitination.
      Title: The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation.
    2. Data indicate the crystal structure of the Ubc7p-binding region (U7BR) of Cue1p with Ubc7p.
      Title: A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms.
    3. Data indicate that efficient formation of longer ubiquitin chains depends on the Cue1p CUE domain.
      Title: Ubiquitin binding by a CUE domain regulates ubiquitin chain formation by ERAD E3 ligases.
    4. Data show that a discrete C-terminal Ubc7p binding region of Cue1p is required for ER-associated degradation and for Ubc7p-dependent ubiquitylation by Hrd1p in vitro.
      Title: A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation.
    5. reveal a previously unobserved stimulation of Ubc7p E2 activity by Cue1p
      Title: Cue1p is an activator of Ubc7p E2 activity in vitro and in vivo.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ubiquitin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-protein transferase activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ERAD pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in establishment of protein localization to endoplasmic reticulum membrane IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Doa10p ubiquitin ligase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of Hrd1p ubiquitin ligase ERAD-L complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum HDA PubMed 
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion HDA PubMed 

    General protein information

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    Preferred Names
    Cue1p
    NP_013991.1
    • Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001145.3 Reference assembly

      Range
      795805..796416
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182771.1 → NP_013991.1  TPA: Cue1p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013991.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W090, P38428, Q03507
      UniProtKB/TrEMBL
      A6ZMX9, B3LMF1, C7GR89, C8ZFD0
      Conserved Domains (2) summary
      cd14424
      Location:67 → 103
      CUE_Cue1p_like; CUE domain found in yeast ubiquitin-binding protein CUE1 (Cue1p), CUE4 (Cue4p) and similar proteins
      pfam18499
      Location:151 → 202
      Cue1_U7BR; Ubc7p-binding region of Cue1
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38428.2

    Gene LinkOut

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