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    ATG4 cysteine protease ATG4 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855498, updated on 9-Dec-2024

    Summary

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    Official Symbol
    ATG4
    Official Full Name
    cysteine protease ATG4
    Primary source
    SGD:S000005167
    Locus tag
    YNL223W
    See related
    AllianceGenome:SGD:S000005167; FungiDB:YNL223W; VEuPathDB:YNL223W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    APG4; AUT2
    Summary
    Enables protein-phosphatidylethanolamide deconjugating activity. Involved in several processes, including cytoplasm to vacuole targeting by the Cvt pathway; piecemeal microautophagy of the nucleus; and protein delipidation. Located in cytoplasm and nucleus. Orthologous to several human genes including ATG4D (autophagy related 4D cysteine peptidase). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See ATG4 in Genome Data Viewer
    Location:
    chromosome: XIV
    Exon count:
    1
    Sequence:
    Chromosome: XIV; NC_001146.8 (227370..228854)

    Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene Cnm67p Neighboring gene Sqs1p Neighboring gene RNA polymerase II subunit A C-terminal domain phosphatase Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001146.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The cysteine protease MoAtg4 interacts with MoAtg8 and is required for differentiation and pathogenesis in Magnaporthe oryzae. Liu TB, et al. Autophagy, 2010 Jan. PMID 19923912
    2. New insights into autophagy using a multiple knockout strain. Cao Y, et al. Autophagy, 2008 Nov. PMID 18971623, Free PMC Article
    3. Autophagy is involved in nutritional stress response and differentiation in Trypanosoma cruzi. Alvarez VE, et al. J Biol Chem, 2008 Feb 8. PMID 18039653
    4. Formation process of autophagosome is traced with Apg8/Aut7p in yeast. Kirisako T, et al. J Cell Biol, 1999 Oct 18. PMID 10525546, Free PMC Article
    5. Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion. Nakatogawa H, et al. Cell, 2007 Jul 13. PMID 17632063

    See all (73) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. findings showt Atg4 is recruited onto autophagosomal membranes by direct binding to Atg8 via two evolutionarily conserved Atg8 recognition sites; propose a model where Atg4 activity on autophagosomal membranes depends on cooperative action of at least 2 sites within Atg4, in which one functions as a constitutive Atg8 binding module, while the other has a preference toward phosphatidylethanolamine-bound Atg8
      Title: Conserved Atg8 recognition sites mediate Atg4 association with autophagosomal membranes and Atg8 deconjugation.
    2. Data show that phosphorylation by Atg1 inhibits Atg4 at autophagosome formation sites.
      Title: Atg4 proteolytic activity can be inhibited by Atg1 phosphorylation.
    3. Results provide evidence that Atg4 plays an important role in efficient expansion of autophagic isolation membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae.
      Title: Atg4 plays an important role in efficient expansion of autophagic isolation membranes by cleaving lipidated Atg8 in Saccharomyces cerevisiae.
    4. fine-tuning of Atg4 activity depending on the intracellular redox state may regulate autophagosome formation
      Title: The yeast autophagy protease Atg4 is regulated by thioredoxin.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables cysteine-type endopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables cysteine-type peptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-phosphatidylethanolamide deconjugating activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables protein-phosphatidylethanolamide deconjugating activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein-phosphatidylethanolamide deconjugating activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein-phosphatidylethanolamide deconjugating activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in C-terminal protein lipidation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in aggrephagy IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in autophagosome assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in autophagy IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in autophagy of mitochondrion IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in cytoplasm to vacuole targeting by the Cvt pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in mitophagy IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in nucleophagy IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in piecemeal microautophagy of the nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in piecemeal microautophagy of the nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein delipidation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in phagophore assembly site IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    cysteine protease ATG4
    NP_014176.2
    • Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001146.8 Reference assembly

      Range
      227370..228854
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183061.1 → NP_014176.2  TPA: cysteine protease ATG4 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014176.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      A6ZRL7, D6W0W7, P53867
      UniProtKB/TrEMBL
      B3LP67, C7GPD4, N1NX11
      Conserved Domains (1) summary
      pfam03416
      Location:84 → 371
      Peptidase_C54; Peptidase family C54
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P53867.2

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