RCF2 Rcf2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RCF2
Official Full Name: Rcf2p
Primary source: SGD:S000005301
Locus tag: YNR018W
See related: AllianceGenome:SGD:S000005301; FungiDB:YNR018W; VEuPathDB:YNR018W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AIM38
Summary: Involved in mitochondrial cytochrome c oxidase assembly. Acts upstream of or within positive regulation of protein-containing complex assembly. Located in mitochondrial inner membrane. Part of respiratory chain complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RCF2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (664270..664944)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rcf proteins and their differential specificity for respiratory chain complexes: A unique role for Rcf2 on oxygen sensitive supercomplexes?
Title: Rcf proteins and their differential specificity for respiratory chain complexes: A unique role for Rcf2 on oxygen sensitive supercomplexes?
NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.
Title: NMR Structure and Dynamics Studies of Yeast Respiratory Supercomplex Factor 2.
Using yeast as a model organism, we have shown previously that its two HIGD1 proteins, Rcf1 and Rcf2, are required for the generation and maintenance of a normal membrane potential (DeltaPsi) across the inner mitochondrial membrane (IMM). We postulated that the lower DeltaPsi observed in the absence of the HIGD1 proteins may be due to decreased proton pumping by complex IV (CIV) or enhanced leak of protons across the IMM
Title: Hypoxia-inducible gene domain 1 proteins in yeast mitochondria protect against proton leak through complex IV.
the absence of Rcf1 and Rcf2 proteins on the CIV complex are distinct in terms of CIV assembly/accumulation and additive in terms of the ability of CIV to generate PMF. Thus, the combined absence of Rcf1 and Rcf2 alters both CIV physiology and assembly.
Title: The yeast mitochondrial proteins Rcf1 and Rcf2 support the enzymology of the cytochrome c oxidase complex and generation of the proton motive force.
The secondary structure of Rcf2 contains seven helices, of which five are putative transmembrane (TM) helices, including, unexpectedly, a region formed by a charged 20-residue helix at the C terminus. These studies demonstrated that Rcf2 forms a dimer, and the charged TM helix is involved in this dimer formation.
Title: NMR Study of Rcf2 Reveals an Unusual Dimeric Topology in Detergent Micelles.
Data suggest the regulatory mechanism of the respiratory supercomplex factors 1 and 2 (Rcf1 and Rcf2) in the context of supramolecular interactions between cyt. bc1 and CytcO.
Title: Regulatory role of the respiratory supercomplex factors in Saccharomyces cerevisiae.
The study reports on the maturation of Rcf2 into an N- and C-terminal peptide. It shows that the previously uncharacterized Rcf3 (YBR255c-A) is a homolog of the N-terminal Rcf2 peptide, whereas Rcf1 is homologous to the C-terminal portion. Both Rcf3 and the C-terminal fragment of Rcf2 associate with monomeric cytochrome c oxidase and respiratory chain supercomplexes.
Title: Overlapping Role of Respiratory Supercomplex Factor Rcf2 and Its N-terminal Homolog Rcf3 in Saccharomyces cerevisiae.
Rcf1 displays overlapping function with another Hig1-related protein, Rcf2 (formerly Aim38), and their joint presence is required for optimal COX enzyme activity and the correct assembly of the cytochrome bc(1)-COX supercomplex
Title: Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in mitochondrial cytochrome c oxidase assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial cytochrome c oxidase assembly	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within positive regulation of protein-containing complex assembly	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
part_of respiratory chain complex	IBA Inferred from Biological aspect of Ancestor more info
part_of respiratory chain complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rcf2p

NP_014415.1

Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; antagonistic with Min8p to control ETC assembly member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast