AFT2 Aft2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: AFT2
Official Full Name: Aft2p
Primary source: SGD:S000006123
Locus tag: YPL202C
See related: AllianceGenome:SGD:S000006123; FungiDB:YPL202C; VEuPathDB:YPL202C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and iron-sulfur cluster binding activity. Involved in several processes, including cardiolipin metabolic process; cellular response to iron ion starvation; and regulation of phosphate transmembrane transport. Located in mitochondrion. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See AFT2 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (168088..169338, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The conserved CDC motif in the yeast iron regulator Aft2 mediates iron-sulfur cluster exchange and protein-protein interactions with Grx3 and Bol2
Title: The conserved CDC motif in the yeast iron regulator Aft2 mediates iron-sulfur cluster exchange and protein-protein interactions with Grx3 and Bol2.
Comparative affinity assays indicated that the interaction between Fra2 and Aft2 is much stronger than that between Grx3 and Aft2, or Aft2 toward its target DNA. Aft2 activity seems to be regulated by Grx3.
Title: Structural and Biochemical Insights into the Multiple Functions of Yeast Grx3.
the absence of Aft2 leads to enhanced Pho90 function, involving both Spl2-dependent and independent events and resulting in selenite hyperaccumulation and toxicity.
Title: The yeast Aft2 transcription factor determines selenite toxicity by controlling the low affinity phosphate transport system.
Two Aft2 monomers bind a [2Fe-2S] cluster (or Fe2+) through a Cys-Asp-Cys motif, leading to dimerization of Aft2 and decreased DNA-binding affinity.
Title: Molecular mechanism and structure of the Saccharomyces cerevisiae iron regulator Aft2.
Aft2 activates the transcription of genes involved in intracellular iron use in the absence of Aft1.
Title: Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1.
Aft1/Aft2 activation of the iron regulon depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis
Title: Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables iron-sulfur cluster binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cardiolipin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to iron ion starvation	IEA Inferred from Electronic Annotation more info
involved_in cellular response to iron ion starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in intracellular iron ion homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of phosphate transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Aft2p

NP_015122.1

Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication