CTF4 chromatin-binding protein CTF4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CTF4
Official Full Name: chromatin-binding protein CTF4
Primary source: SGD:S000006339
Locus tag: YPR135W
See related: AllianceGenome:SGD:S000006339; FungiDB:YPR135W; VEuPathDB:YPR135W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CHL15; POB1
Summary: Enables chromatin binding activity. Involved in DNA metabolic process and sister chromatid cohesion. Located in nuclear replication fork. Part of replication fork protection complex. Human ortholog(s) of this gene implicated in cholangiocarcinoma. Orthologous to human WDHD1 (WD repeat and HMG-box DNA binding protein 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CTF4 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (799234..802017)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.
Title: Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.
Here the authors show that Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, Ctf4 binds only one Pol alpha-primase. Thus, Ctf4 may have evolved as a trimer to organize two helicases and one Pol alpha-primase into a replication factory.
Title: Ctf4 organizes sister replisomes and Pol α into a replication factory.
ur results support a model whereby parental (H3-H4)2 complexes displaced from nucleosomes by DNA unwinding at replication forks are transferred by the CMG-Ctf4-Polalpha complex to lagging-strand DNA for nucleosome assembly at the original location.
Title: The Mcm2-Ctf4-Polα Axis Facilitates Parental Histone H3-H4 Transfer to Lagging Strands.
Ctf4 as a key regulator of DNA double strand end resection at arrested forks. End resection also occurs during physiological rDNA amplification even in the presence of Ctf4. Suppression of end resection is thus important for protecting DSBs at arrested forks from chromosome rearrangements.
Title: Ctf4 Prevents Genome Rearrangements by Suppressing DNA Double-Strand Break Formation and Its End Resection at Arrested Replication Forks.
The Chl1 helicase facilitates replication fork progression under conditions of nucleotide depletion, partly independently of Ctf4 interaction.
Title: Ctf4 Links DNA Replication with Sister Chromatid Cohesion Establishment by Recruiting the Chl1 Helicase to the Replisome.
Ctf4-dependent recruitment of Dna2/Tof2 couples other processes to DNA synthesis, including rDNA copy-number regulation.
Title: Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.
Ctf4 is an essential member of the H3 lysine 56 acetylation pathway.
Title: Replisome function during replicative stress is modulated by histone h3 lysine 56 acetylation through Ctf4.
Data indicate that a defective DNA polymerase alpha (Polalpha)/DNA primase/chromatin-binding protein Ctf4 complex causes unscheduled strand annealing and fork reversal.
Title: Error-free DNA damage tolerance and sister chromatid proximity during DNA replication rely on the Polα/Primase/Ctf4 Complex.
Ctf4 can couple two molecules of Pol alpha to one CMG helicase within the replisome, providing a new model for lagging-strand synthesis in eukaryotes that resembles the emerging model for the simpler replisome of Escherichia coli
Title: A Ctf4 trimer couples the CMG helicase to DNA polymerase α in the eukaryotic replisome.
These data indicate that Ctf4p facilitates Mcm10p to promote the DNA replication.
Title: Ctf4p facilitates Mcm10p to promote DNA replication in budding yeast.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA-templated DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA-templated DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in nuclear replication fork	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: chromatin-binding protein CTF4

NP_015461.1

Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p); recruits Mms22p to replication forks during S phase, and may link DNA synthesis to sister chromatid cohesion

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

799234..802017

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184232.1 → NP_015461.1 TPA: chromatin-binding protein CTF4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015461.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4D2, Q01454

UniProtKB/TrEMBL

A6ZX13, B3LK97, B5VTS1, N1NWI5

Conserved Domains (3) summary

sd00039
Location:140 → 176

7WD40; WD40 repeat [structural motif]

pfam12341
Location:478 → 779

Mcl1_mid; Minichromosome loss protein, Mcl1, middle region

cl29593
Location:233 → 347

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...