pum pumilio [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pumprovided by FlyBase
Official Full Name: pumilioprovided by FlyBase
Primary source: FLYBASE:FBgn0003165
Locus tag: Dmel_CG9755
See related: AllianceGenome:FB:FBgn0003165
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0172774.19; bem; CG9755; CG9763; Dmel\CG9755; EP(3)0883; fs(3)02003; l(3)01688; ova; ovt; pkl; PKL; Pum; PUM
Summary: Enables CCR4-NOT complex binding activity and nucleic acid binding activity. Involved in several processes, including behavioral response to ethanol; head involution; and positive regulation of mRNA catabolic process. Located in neuronal ribonucleoprotein granule and nuclear envelope. Is active in muscle cell postsynaptic specialization; type Ib terminal bouton; and type Is terminal bouton. Is expressed in several structures, including adult brain cell body rind; adult head; border follicle cell; cuboidal/columnar epithelium; and germline cell. Human ortholog(s) of this gene implicated in cerebellar ataxia type 47. Orthologous to several human genes including PUM2 (pumilio RNA binding family member 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Location:: 85C4-85D1; 3-48 cM

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (9066343..9237682, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (4892065..5063404, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of the Drosophila transcriptome by Pumilio and the CCR4-NOT deadenylase complex.
Title: Regulation of the Drosophila transcriptome by Pumilio and the CCR4-NOT deadenylase complex.
A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs.
Title: A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs.
Structure and dynamics of the quaternary hunchback mRNA translation repression complex.
Title: Structure and dynamics of the quaternary hunchback mRNA translation repression complex.
Myocyte enhancer factor-2 and p300 interact to regulate the expression of homeostatic regulator Pumilio in Drosophila.
Title: Myocyte enhancer factor-2 and p300 interact to regulate the expression of homeostatic regulator Pumilio in Drosophila.
Findings identify Bag-of-marbles (Bam) as a new pumilio (Pum) cofactor in repression of Mothers against dpp (mad) 3' UTR in Schneider's 2 cells (S2 cells).
Title: The target specificity of the RNA binding protein Pumilio is determined by distinct co-factors.
This study explores the crystallization and structure determination of a Pum-Nos-RNA complex.
Title: Preparation of cooperative RNA recognition complexes for crystallographic structural studies.
the catalytic activity of Pop2 deadenylase is important for Pumilio RD activity. Further, we show that the Pumilio RDs directly bind to the CNOT complex. We also report that the decapping enzyme, Dcp2, participates in repression by the N-terminus of Pumilio. These results support a model wherein Pumilio utilizes CNOT deadenylase and decapping complexes to accelerate destruction of target mRNAs.
Title: Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs.
Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor proteins has been described.
Title: Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor Proteins.
The specificity of a complex between Drosophila RNA-binding protein Pumilio and its target RNA of Nanos has been described.
Title: Integrated analysis of RNA-binding protein complexes using in vitro selection and high-throughput sequencing and sequence specificity landscapes (SEQRS).
Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity.
Title: Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Markers

Dm0358 (e-PCR)
- UniSTS:82309

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables CCR4-NOT complex binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA 3'-UTR binding	TAS Traceable Author Statement more info	PubMed
enables mRNA regulatory element binding translation repressor activity	IDA Inferred from Direct Assay more info	PubMed
enables mRNA regulatory element binding translation repressor activity	TAS Traceable Author Statement more info	PubMed
enables sequence-specific mRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in anterior/posterior axis specification, embryo	TAS Traceable Author Statement more info	PubMed
involved_in behavioral response to ethanol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	TAS Traceable Author Statement more info	PubMed
involved_in chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell development	TAS Traceable Author Statement more info	PubMed
involved_in germ cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ cell migration	TAS Traceable Author Statement more info	PubMed
involved_in head involution	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term memory	TAS Traceable Author Statement more info	PubMed
involved_in mitotic cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-templated transcription	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of cell cycle	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of epidermal growth factor receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of translation	TAS Traceable Author Statement more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	TAS Traceable Author Statement more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of deadenylation-independent decapping of nuclear-transcribed mRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of translation	TAS Traceable Author Statement more info	PubMed
involved_in post-transcriptional gene silencing	IDA Inferred from Direct Assay more info	PubMed
involved_in post-transcriptional regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of synaptic assembly at neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in muscle cell postsynaptic specialization	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal ribonucleoprotein granule	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in type Ib terminal bouton	IDA Inferred from Direct Assay more info	PubMed
is_active_in type Is terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

Go to the top of the page Help

Preferred Names: pumilio

Names: CG9755-PA; CG9755-PB; CG9755-PC; CG9755-PD; CG9755-PE; CG9755-PF; CG9755-PG; CG9755-PH; PUMILIO; bemused; milord; ovarette; pum-PA; pum-PB; pum-PC; pum-PD; pum-PE; pum-PF; pum-PG; pum-PH; pumillio; pumuckel