Prkci protein kinase C, iota [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Prkciprovided by RGD
Official Full Name: protein kinase C, iotaprovided by RGD
Primary source: RGD:620961
See related: EnsemblRapid:ENSRNOG00000009652 AllianceGenome:RGD:620961
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pkcl
Summary: Predicted to enable diacylglycerol-dependent serine/threonine kinase activity and phospholipid binding activity. Involved in several processes, including Golgi vesicle budding; cellular response to insulin stimulus; and response to interleukin-1. Located in bicellular tight junction and cell leading edge. Is active in Schaffer collateral - CA1 synapse and glutamatergic synapse. Orthologous to human PRKCI (protein kinase C iota). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Lung (RPKM 259.4), Kidney (RPKM 161.9) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 2q24

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	2	NC_086020.1 (114250398..114310784, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	2	NC_051337.1 (112321919..112382305, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	2	NC_005101.4 (115941998..116002550, complement)

Chromosome 2 - NC_086020.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PKMzeta, but not PKCiota, knock-down disrupted previously established long-time memory. PKCiota and PKMz have distinct functions in long-term potentiation and memory, with PKMzeta playing a specific role in memory maintenance.
Title: Distinct Roles of PKCι/λ and PKMζ in the Initiation and Maintenance of Hippocampal Long-Term Potentiation and Memory.
Polarity signaling via CDC42/atypical protein kinase C can affect the dynamic turnover of the intermediate filament network to promote the polarization of the network itself.
Title: Regulation of microtubule-associated motors drives intermediate filament network polarization.
Acute inhibition of CNS aPKC activity increases food intake and glucose intolerance. Chronic inactivation of CNS aPKC activity increases body weight and food intake during high fat diet feeding.
Title: Deletion of Protein Kinase C λ in POMC Neurons Predisposes to Diet-Induced Obesity.
FGF21 inhibits hepatic glucose production by the PI3K-dependent activation of protein kinase c iota.
Title: FGF21 suppresses hepatic glucose production through the activation of atypical protein kinase Cι/λ.
PKClambda is critical in AMPA receptor phosphorylation and synaptic incorporation during long-term potentiation.
Title: PKCλ is critical in AMPA receptor phosphorylation and synaptic incorporation during LTP.
inactivity-induced increases in phrenic inspiratory output require local PKCzeta/iota activity to stabilize into a long-lasting phrenic motor facilitation
Title: Spinal atypical protein kinase C activity is necessary to stabilize inactivity-induced phrenic motor facilitation.
Protein kinase C-lambda gene knockdown modulates hypothalamic glutathione peroxidase, melanocortin receptor and neuropeptide Y gene expression in amphetamine-treated rats.
Title: Knocking down the transcript of protein kinase C-lambda modulates hypothalamic glutathione peroxidase, melanocortin receptor and neuropeptide Y gene expression in amphetamine-treated rats.
Results show that although hypothalamic aPKC signaling is not required for food intake inhibition by insulin or leptin, it plays a key role in inflammatory anorexia and fever induced by LPS.
Title: Atypical protein kinase C activity in the hypothalamus is required for lipopolysaccharide-mediated sickness responses.
Data show that the migration associated control of JNK by aPKCs determines JNK phosphorylation of the plasma membrane substrate paxillin.
Title: An aPKC-exocyst complex controls paxillin phosphorylation and migration through localised JNK1 activation.
Report involvement of phosphatidylinositol-3 kinase/AKT/PKCzeta/lambda pathway in the effect of palmitate on glucose-induced insulin secretion.
Title: Involvement of phosphatidylinositol-3 kinase/AKT/PKCzeta/lambda pathway in the effect of palmitate on glucose-induced insulin secretion.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI007955 (e-PCR)
- UniSTS:261720

RH143812 (e-PCR)
- UniSTS:233058

AI406627 (e-PCR)
- UniSTS:250036

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Golgi vesicle budding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-cell junction organization	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to insulin stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within establishment of apical/basal cell polarity	ISO Inferred from Sequence Orthology more info
involved_in establishment of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of epithelial cell apical/basal polarity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within eye photoreceptor cell development	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glial cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of D-glucose import	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of glial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glial cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron projection development	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	EXP Inferred from Experiment more info	PubMed
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of postsynaptic membrane neurotransmitter receptor levels	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to interleukin-1	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to peptide hormone	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
part_of PAR polarity complex	ISO Inferred from Sequence Orthology more info
is_active_in Schaffer collateral - CA1 synapse	EXP Inferred from Experiment more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in Schmidt-Lanterman incisure	ISO Inferred from Sequence Orthology more info
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in brush border	ISO Inferred from Sequence Orthology more info
located_in cell leading edge	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in endosome	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	EXP Inferred from Experiment more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in intercellular bridge	IEA Inferred from Electronic Annotation more info
located_in intercellular bridge	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: protein kinase C iota type

Names: aPKC-lambda/iota; atypical protein kinase C-lambda/iota; nPKC-iota; protein kinase C, lambda

NP_114448.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_032059.1 → NP_114448.1 protein kinase C iota type

See identical proteins and their annotated locations for NP_114448.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000002

UniProtKB/Swiss-Prot

B1PZT8, F1M7Y5

Related

ENSRNOP00000012924.4, ENSRNOT00000012924.7

Conserved Domains (3) summary

cd06404
Location:26 → 108

PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...

cd05618
Location:233 → 596

STKc_aPKC_iota; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota

cd20794
Location:139 → 193

C1_aPKC; protein kinase C conserved region 1 (C1 domain) found in the atypical protein kinase C (aPKC) family