Oaz1 ornithine decarboxylase antizyme 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Oaz1provided by RGD
Official Full Name: ornithine decarboxylase antizyme 1provided by RGD
Primary source: RGD:3219
See related: EnsemblRapid:ENSRNOG00000019459 AllianceGenome:RGD:3219
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Oaz; ODCAC; ODC-Az
Summary: The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014]
Expression: Biased expression in Muscle (RPKM 2001.1), Heart (RPKM 1859.0) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q11

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (9534530..9536990, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (8883855..8886315, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (11752127..11754587, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

antizyme expression requires programmed,+1 ribosomal frameshifting
Title: Autoregulatory frameshifting in decoding mammalian ornithine decarboxylase antizyme.
Corticosterone treatment induces antizyme 1 protein synthesis in early regenerating rat liver. However, it has little effect on antizyme 1 gene transcription.
Title: The influence of corticosterone on antizyme gene expression in early regenerating rat liver.
overexpression of ornithine decarboxylase antizyme 1 is necessary for conversion of pancreatic tumor cells into glucagon-producing differentiated cells.
Title: Antizyme is necessary for conversion of pancreatic tumor cells into glucagon-producing differentiated cells.
ability of antizyme 1 to shuttle between the nucleus and the cytoplasm suggests that it has a novel function in the nucleus
Title: Identification of nuclear export signals in antizyme-1.
Degradation of antizyme requires a functional ubiquitin system.
Title: Ornithine decarboxylase-antizyme is rapidly degraded through a mechanism that requires functional ubiquitin-dependent proteolytic activity.
NMR study of the solution structure of a stable, folded domain of antizyme isoform-1 consisting of amino acid residues 87-227; it contains eight beta strands & two alpha helices with the strands forming a mixed parallel & antiparallel beta sheet
Title: Solution structure of a conserved domain of antizyme: a protein regulator of polyamines.
The antizyme 1-mediated pathway for cyclin D1 degradation is independent of the previously characterized phosphorylation- and ubiquitination-dependent pathway
Title: Antizyme targets cyclin D1 for degradation. A novel mechanism for cell growth repression.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Oaz1 (e-PCR), detects polymorphism
- UniSTS:531478

RH142210 (e-PCR)
- UniSTS:231460

RH127959 (e-PCR)
- UniSTS:211269

Oaz1 (e-PCR), detects polymorphism
- UniSTS:144075

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables enzyme binding	IDA Inferred from Direct Assay more info	PubMed
enables ornithine decarboxylase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables ornithine decarboxylase inhibitor activity	IDA Inferred from Direct Assay more info	PubMed
enables ornithine decarboxylase inhibitor activity	IEA Inferred from Electronic Annotation more info
enables ornithine decarboxylase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to leptomycin B	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to oxygen-glucose deprivation	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to polyamine macromolecule	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of ornithine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of polyamine transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of polyamine transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in polyamine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of intracellular protein transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intracellular protein transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of proteasomal protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to ischemia	IEP Inferred from Expression Pattern more info	PubMed
involved_in viral translational frameshifting	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ornithine decarboxylase antizyme 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001297557.1 → NP_001284486.1 ornithine decarboxylase antizyme 1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternative in-frame translation initiation codons. The longer isoform (1, also known as 29 kDa form or AZ-1) is derived from an upstream AUG (at nt 80-82), whereas the shorter isoform (2, also known as 24.5 kDa form or AZ-2) is derived from a downstream AUG (at nt 179-181). This RefSeq represents the shorter isoform, which is found in the cytosol (PMID:16120325).

Source sequence(s)

FQ216817

Conserved Domains (1) summary

pfam02100
Location:106 → 185

ODC_AZ; Ornithine decarboxylase antizyme
NM_001301048.1 → NP_001287977.1 ornithine decarboxylase antizyme 1 isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate, in-frame acceptor splice site at the second exon compared to variant 1. The resulting isoform (3) lacks 2 internal amino acids compared to isoform 1.

Source sequence(s)

FQ216817

Conserved Domains (1) summary

pfam02100
Location:137 → 216

ODC_AZ; Ornithine decarboxylase antizyme
NM_139081.2 → NP_620781.1 ornithine decarboxylase antizyme 1 isoform 1

See identical proteins and their annotated locations for NP_620781.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternative in-frame translation initiation codons. The longer isoform (1, also known as 29 kDa form or AZ-1) is derived from an upstream AUG (at nt 80-82), whereas the shorter isoform (2, also known as 24.5 kDa form or AZ-2) is derived from a downstream AUG (at nt 179-181). This RefSeq represents the longer isoform, which is localized to the mitochondria (PMID:16120325).

Source sequence(s)

FQ216817

UniProtKB/Swiss-Prot

P54370

Related

ENSRNOP00000090905.2, ENSRNOT00000110971.2

Conserved Domains (1) summary

pfam02100
Location:139 → 218

ODC_AZ; Ornithine decarboxylase antizyme