Sun5 Sad1 and UNC84 domain containing 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sun5provided by MGI
Official Full Name: Sad1 and UNC84 domain containing 5provided by MGI
Primary source: MGI:MGI:1923657
See related: Ensembl:ENSMUSG00000027480 AllianceGenome:MGI:1923657
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SRG4; Spag4l; SPAG4L-2; 1700021O15Rik
Summary: Predicted to enable protein-membrane adaptor activity. Involved in spermatid development. Acts upstream of or within protein-containing complex assembly; regulation of protein localization; and spermatogenesis. Located in sperm head-tail coupling apparatus. Human ortholog(s) of this gene implicated in spermatogenic failure 16. Orthologous to human SUN5 (Sad1 and UNC84 domain containing 5). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward testis adult (RPKM 58.2) See more
Orthologs: human all
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Genomic context

Location:: 2 H1; 2 75.99 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (153698108..153713281, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (153856188..153871365, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.
Title: The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.
These studies reveal the essential role of SUN5 in anchoring sperm head to the tail and provide a promising way to treat this kind of acephalic spermatozoa-associated male infertility.
Title: Essential role for SUN5 in anchoring sperm head to the tail.
In Dpy19l2 knock-out round spermatids, the detachment of the acrosome leads to Sun5 and lamin B1 relocalization, confirming that Sun5 protein is excluded from the nuclear envelope facing the acrosome.
Title: Dynamics of Sun5 localization during spermatogenesis in wild type and Dpy19l2 knock-out mice indicates that Sun5 is not involved in acrosome attachment to the nuclear envelope.
SPAG4L may play an important role in the meiotic stage of spermatogenesis
Title: SPAG4L, a novel nuclear envelope protein involved in the meiotic stage of spermatogenesis.
SPAG4L-2 and SPAG4L are transmembrane proteins that localize to the nuclear envelope.
Title: SPAG4L/SPAG4L-2 are testis-specific SUN domain proteins restricted to the apical nuclear envelope of round spermatids facing the acrosome.
SRG4 was cloned into pUCm-T vectors and sequenced, subcloned into a prokaryotic expression vector and expression of induced by IPTG.
Title: [Prokaryotic expression and purification of SRG4, a novel mouse spermatogenesis gene].
Results indicated that the expression of SRG4 was regulated by development, and SRG4 was mainly expressed in the cytoplasm of spermatocytes and round spermatids.
Title: [Expression of a novel spermatogenesis gene SRG4 in postpartum normal and cryptorchid mouse testis].

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:490701

NoName (e-PCR)
- UniSTS:488943

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-membrane adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
part_of meiotic nuclear membrane microtubule tethering complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear inner membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear inner membrane	ISO Inferred from Sequence Orthology more info
is_active_in sperm head-tail coupling apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in sperm head-tail coupling apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in sperm head-tail coupling apparatus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: SUN domain-containing protein 5

Names: sperm-associated antigen 4-like protein; spermatogenesis-related gene 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001305048.2 → NP_001291977.1 SUN domain-containing protein 5 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AL833803

UniProtKB/Swiss-Prot

D2DR64, Q5DT38, Q9DA32

Conserved Domains (1) summary

pfam07738
Location:229 → 361

Sad1_UNC; Sad1 / UNC-like C-terminal
NM_029599.3 → NP_083875.1 SUN domain-containing protein 5 isoform 2

See identical proteins and their annotated locations for NP_083875.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.

Source sequence(s)

AL833803

Consensus CDS

CCDS38288.1

UniProtKB/Swiss-Prot

D2DR64, Q5DT38, Q9DA32

UniProtKB/TrEMBL

A0A0R4J084

Related

ENSMUSP00000028982.5, ENSMUST00000028982.5

Conserved Domains (1) summary

pfam07738
Location:204 → 336

Sad1_UNC; Sad1 / UNC-like C-terminal

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

153698108..153713281 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252189.2 → XP_030108049.1 SUN domain-containing protein 5 isoform X3

UniProtKB/Swiss-Prot

D2DR64, Q5DT38, Q9DA32

Conserved Domains (1) summary

pfam07738
Location:170 → 302

Sad1_UNC; Sad1 / UNC-like C-terminal
XM_006500372.2 → XP_006500435.2 SUN domain-containing protein 5 isoform X1

UniProtKB/Swiss-Prot

D2DR64, Q5DT38, Q9DA32

Conserved Domains (1) summary

pfam07738
Location:246 → 378

Sad1_UNC; Sad1 / UNC-like C-terminal
XM_006500374.1 → XP_006500437.1 SUN domain-containing protein 5 isoform X2

UniProtKB/Swiss-Prot

D2DR64, Q5DT38, Q9DA32

Conserved Domains (1) summary

pfam07738
Location:213 → 345

Sad1_UNC; Sad1 / UNC-like C-terminal
XM_006500375.2 → XP_006500438.1 SUN domain-containing protein 5 isoform X4

Conserved Domains (1) summary

pfam07738
Location:115 → 247

Sad1_UNC; Sad1 / UNC-like C-terminal
XM_011239838.1 → XP_011238140.1 SUN domain-containing protein 5 isoform X5

Conserved Domains (1) summary

cl00240
Location:154 → 214

RRF; Ribosome recycling factor (RRF). Ribosome recycling factor dissociates the posttermination complex, composed of the ribosome, deacylated tRNA, and mRNA, after termination of translation. Thus ribosomes are "recycled" and ready for another round of ...