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    Masp2 MBL associated serine protease 2 [ Rattus norvegicus (Norway rat) ]

    Gene ID: 64459, updated on 27-Nov-2024

    Summary

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    Official Symbol
    Masp2provided by RGD
    Official Full Name
    MBL associated serine protease 2provided by RGD
    Primary source
    RGD:620214
    See related
    EnsemblRapid:ENSRNOG00000011258 AllianceGenome:RGD:620214
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Rattus norvegicus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
    Also known as
    MAp19; MASP-2
    Summary
    Enables serine-type endopeptidase activity. Predicted to be involved in complement activation, lectin pathway. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Orthologous to human MASP2 (MBL associated serine protease 2). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Biased expression in Liver (RPKM 632.8), Kidney (RPKM 47.4) and 4 other tissues See more
    Orthologs
    human mouse all
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    Genomic context

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    See Masp2 in Genome Data Viewer
    Location:
    5q36
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCr8 (GCF_036323735.1) 5 NC_086023.1 (164319017..164332686)
    RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 5 NC_051340.1 (159035892..159049561)
    106 previous assembly Rnor_6.0 (GCF_000001895.5) 5 NC_005104.4 (165415105..165429857)

    Chromosome 5 - NC_086023.1Genomic Context describing neighboring genes Neighboring gene exosome component 10 Neighboring gene spermidine synthase Neighboring gene TAR DNA binding protein Neighboring gene small nucleolar RNA, H/ACA box 21 Neighboring gene similar to human chromosome 1 open reading frame 127 Neighboring gene ribosomal protein L14, pseudogene 2

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
    • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
    • BioProject: PRJNA238328
    • Publication: PMID 24510058
    • Analysis date: Mon Jun 6 17:44:12 2016

    Bibliography

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    Related articles in PubMed

    1. Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2. Feinberg H, et al. EMBO J, 2003 May 15. PMID 12743029, Free PMC Article
    2. Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation. Nan R, et al. Structure, 2017 Feb 7. PMID 28111019, Free PMC Article
    3. Two constituents of the initiation complex of the mannan-binding lectin activation pathway of complement are encoded by a single structural gene. Stover CM, et al. J Immunol, 1999 Mar 15. PMID 10092804
    4. The rat and mouse homologues of MASP-2 and MAp19, components of the lectin activation pathway of complement. Stover CM, et al. J Immunol, 1999 Dec 15. PMID 10586086
    5. Decoupling of carbohydrate binding and MASP-2 autoactivation in variant mannose-binding lectins associated with immunodeficiency. Wallis R, et al. J Immunol, 2005 Nov 15. PMID 16272342

    See all (23) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Flexibility in MASP-1 and MASP-2 provides insight on lectin pathway activation.
      Title: Flexibility in Mannan-Binding Lectin-Associated Serine Proteases-1 and -2 Provides Insight on Lectin Pathway Activation.
    2. MASP-1 probably enhances complement activation triggered by MBP.MASP-2 complexes, but it cannot initiate activation itself
      Title: Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases.
    3. crystal structure of the MASP-2 CUB1-EGF-CUB2 dimer reveals an elongated structure with a prominent concave surface that is proposed to be the mannose-binding protein-binding site
      Title: Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2.
    4. A lysine residue (Lys56) within the conserved motif is essential for MASP-2 binding and probably mediates the specificity of the ficolin-MASP interaction.
      Title: Localization and characterization of the mannose-binding lectin (MBL)-associated-serine protease-2 binding site in rat ficolin-A: equivalent binding sites within the collagenous domains of MBLs and ficolins.
    5. Highly conserved intron sequences. Restricted expression contrasts with the ubiquitous expression of neighboring genes.
      Title: Organization of the MASP2 locus and its expression profile in mouse and rat.
    6. Analysis of mutant MBL . MASP-2 complexes assembled from recombinant components shows that reduced complement activation by mutant MBLs is caused by failure to activate MASP-2 efficiently on binding to a mannan-coated surface.
      Title: Decoupling of carbohydrate binding and MASP-2 autoactivation in variant mannose-binding lectins associated with immunodeficiency.
    7. MASP-2, C4, and C2 and their activation fragments have roles in complement activation via the lectin pathway
      Title: Molecular interactions between MASP-2, C4, and C2 and their activation fragments leading to complement activation via the lectin pathway.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    General gene information

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    Markers

    Homology

    Gene Ontology Provided by RGD

    Function Evidence Code Pubs
    enables calcium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables calcium-dependent protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables calcium-dependent protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables complement component C4b binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables complement component C4b binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables identical protein binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables peptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables peptidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables serine-type endopeptidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables serine-type endopeptidase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables serine-type endopeptidase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables serine-type endopeptidase activity ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    involved_in complement activation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in complement activation, classical pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in complement activation, lectin pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in complement activation, lectin pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in complement activation, lectin pathway ISO
    Inferred from Sequence Orthology
    more info
    		  
    involved_in proteolysis IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in extracellular region IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in extracellular space IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    mannan-binding lectin serine protease 2
    Names
    mannan-binding lectin serine peptidase 2
    mannose binding lectin-associated serine protease-2
    mannose-binding protein-associated serine protease 2
    NP_742040.1
    XP_006239462.1
    XP_006239463.1
    XP_063144421.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001429904.1 → NP_001416833.1  mannan-binding lectin serine protease 2 isoform 2 precursor

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005

    2. NM_001429905.1 → NP_001416834.1  mannan-binding lectin serine protease 2 isoform 3 precursor

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005
      UniProtKB/TrEMBL
      A0A0G2K392, A6IU76

    3. NM_172043.2 → NP_742040.1  mannan-binding lectin serine protease 2 isoform 1 precursor

      See identical proteins and their annotated locations for NP_742040.1

      Status: VALIDATED

      Source sequence(s)
      JAXUCZ010000005
      UniProtKB/Swiss-Prot
      Q9JJP3, Q9JJS8, Q9QX83, Q9QX84, Q9QX85, Q9QX86, Q9QX87, Q9QX88, Q9QX89, Q9QX90, Q9QX91, Q9QXD4, Q9WUZ0
      UniProtKB/TrEMBL
      A2VCV7, F7FHD3
      Related
      ENSRNOP00000016317.4, ENSRNOT00000016317.8
      Conserved Domains (5) summary
      smart00020
      Location:443 → 678
      Tryp_SPc; Trypsin-like serine protease
      cd00041
      Location:184 → 295
      CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.
      cd00190
      Location:444 → 681
      Tryp_SPc; Trypsin-like serine protease; Many of these are synthesized as inactive precursor zymogens that are cleaved during limited proteolysis to generate their active forms. Alignment contains also inactive enzymes that have substitutions of the catalytic triad ...
      pfam00084
      Location:300 → 361
      Sushi; Sushi repeat (SCR repeat)
      pfam14670
      Location:152 → 180
      FXa_inhibition; Coagulation Factor Xa inhibitory site

    RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCr8

    Genomic

    1. NC_086023.1 Reference GRCr8

      Range
      164319017..164332686
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_063288351.1 → XP_063144421.1  mannan-binding lectin serine protease 2 isoform X1

    2. XM_006239400.5 → XP_006239462.1  mannan-binding lectin serine protease 2 isoform X2

      Conserved Domains (2) summary
      cd00041
      Location:184 → 295
      CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.
      pfam14670
      Location:152 → 180
      FXa_inhibition; Coagulation Factor Xa inhibitory site

    3. XM_006239401.4 → XP_006239463.1  mannan-binding lectin serine protease 2 isoform X3

      Conserved Domains (2) summary
      cd00041
      Location:19 → 136
      CUB; CUB domain; extracellular domain; present in proteins mostly known to be involved in development; not found in prokaryotes, plants and yeast.
      pfam14670
      Location:152 → 180
      FXa_inhibition; Coagulation Factor Xa inhibitory site
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9JJS8.2 GenPept UniProtKB/Swiss-Prot:Q9JJS8

    Gene LinkOut

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