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    hyaA hydrogenase 1 small subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945579, updated on 17-Dec-2024

    Summary

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    Official Symbol
    hyaA
    Official Full Name
    hydrogenase 1 small subunit
    Primary source
    ECOCYC:EG10468
    Locus tag
    b0972
    See related
    ASAP:ABE-0003286
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0963
    Summary
    Verified Tat substrate: [More information is available at EcoGene: EG10468]. HyaA is the small subunit of hydrogenase 1. [More information is available at EcoCyc: EG10468].
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    Genomic context

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    Sequence:
    NC_000913.3 (1032139..1033257)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene modulator of FtsH protease Neighboring gene tRNA-Ser Neighboring gene hydrogenase 1 large subunit Neighboring gene hydrogenase 1 cytochrome b subunit Neighboring gene putative hydrogenase 1 maturation protease HyaD Neighboring gene putative HyaA chaperone Neighboring gene protein HyaF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. X-ray structural, functional and computational studies of the O(2)-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster. Volbeda A , et al. Chem Commun (Camb), 2018 Jun 26. PMID 29888350
    2. Assembly of membrane-bound respiratory complexes by the Tat protein-transport system. Sargent F, et al. Arch Microbiol, 2002 Aug. PMID 12115052
    3. Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12. Pinske C, et al. Microbiology (Reading), 2012 Mar. PMID 22241049
    4. A signal peptide that directs non-Sec transport in bacteria also directs efficient and exclusive transport on the thylakoid Delta pH pathway. Mori H, et al. J Biol Chem, 1998 May 8. PMID 9565548
    5. Importance of the Active Site "Canopy" Residues in an O(2)-Tolerant [NiFe]-Hydrogenase. Brooke EJ, et al. Biochemistry, 2017 Jan 10. PMID 28001048

    See all (52) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The crystal structure of the Escherichia coli O2-sensitive C19G [NiFe]-hydrogenase-1 variant shows that the mutation results in a novel FeS cluster, proximal to the Ni-Fe active site.
      Title: X-ray structural, functional and computational studies of the O(2)-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster.
    2. Studies indicate that Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, and the arginine/lysine substitution lowers the H2 oxidation rate by nearly 2 orders of magnitude.
      Title: Importance of the Active Site "Canopy" Residues in an O(2)-Tolerant [NiFe]-Hydrogenase.
    3. Crystal structure of dimeric membrane-bound O(2)-tolerant hydrogenase 1 from Escherichia coli in a 2:1 complex with its physiological partner, cytochrome b.
      Title: Crystal structure of the O(2)-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b.
    4. a combination of ArcA and AppY antirepression of IscR function, lower levels of IscR, and weak repression by [2Fe-2S]-IscR leads to increased hyaA promoter expression under anaerobic conditions
      Title: ArcA and AppY antagonize IscR repression of hydrogenase-1 expression under anaerobic conditions, revealing a novel mode of O2 regulation of gene expression in Escherichia coli.
    5. These results suggest that the ability to oxidize hydrogen by hydrogenase 1 shows a strong correlation with the rate of carbon flow through glycolysis and provides a direct link between hydrogen, carbon and energy metabolism.
      Title: Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12.
    6. Studies indicate that Hyd-1 and Hyd-2 are two H(2) uptake enzymes, while Hyd-1 is encoded by the first two genes of the hyaABCDEF operon, hyaA and hyaB, Hyd-2 is encoded by genes hybO and hybC of the hybOABCDEFG operon.
      Title: How Escherichia coli is equipped to oxidize hydrogen under different redox conditions.
    7. N-terminus verified by Edman degradation on mature peptide
      Title: Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables electron transfer activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables electron transfer activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ferredoxin hydrogenase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydrogenase (acceptor) activity IC
    Inferred by Curator
    more info
    		 PubMed 
    enables hydrogenase (acceptor) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables iron-sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within anaerobic electron transport chain IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in anaerobic electron transport chain IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in anaerobic respiration IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in anaerobic respiration IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within anaerobic respiration IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in cellular response to starvation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in fermentation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within fermentation IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within hydrogen metabolic process IC
    Inferred by Curator
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of [Ni-Fe] hydrogenase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of [Ni-Fe] hydrogenase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of [Ni-Fe] hydrogenase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of [Ni-Fe] hydrogenase complex RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    part_of ferredoxin hydrogenase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane ISM
    Inferred from Sequence Model
    more info
    		 PubMed 
    located_in periplasmic side of plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    hydrogenase 1 small subunit
    NP_415491.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1032139..1033257
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415491.1 hydrogenase 1 small subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415491.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A6M9WX87
      Conserved Domains (1) summary
      TIGR00391
      Location:2 → 360
      hydA; hydrogenase (NiFe) small subunit (hydA)
    Nucleotide Protein
    Heading Accession and Version

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