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    exbB Ton complex subunit ExbB [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945420, updated on 17-Dec-2024

    Summary

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    Official Symbol
    exbB
    Official Full Name
    Ton complex subunit ExbB
    Primary source
    ECOCYC:EG10271
    Locus tag
    b3006
    See related
    ASAP:ABE-0009869
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2999
    Summary
    ExbB and ExbD proteins function as part of the TonB-dependent energy transduction system for the import of iron-siderophore complexes and vitamin B12 across the outer membrane. [More information is available at EcoCyc: EG10271].
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    Genomic context

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    Sequence:
    NC_000913.3 (3151250..3151984, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene NADP(+)-dependent aldehyde reductase Neighboring gene Ton complex subunit ExbD Neighboring gene cystathionine beta-lyase/L-cysteine desulfhydrase/alanine racemase Neighboring gene DedA family protein YghB

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Excretion of enterochelin by exbA and exbB mutants of Escherichia coli. Guterman SK, et al. J Bacteriol, 1973 Jun. PMID 4576403, Free PMC Article
    2. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. Kampfenkel K, et al. J Biol Chem, 1993 Mar 15. PMID 8449962
    3. ExbB cytoplasmic loop deletions cause immediate, proton motive force-independent growth arrest. Bulathsinghala CM, et al. J Bacteriol, 2013 Oct. PMID 23913327, Free PMC Article
    4. Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway. Baker KR, et al. J Bacteriol, 2013 Jun. PMID 23603742, Free PMC Article
    5. Hexameric and pentameric complexes of the ExbBD energizer in the Ton system. Maki-Yonekura S, et al. Elife, 2018 Apr 17. PMID 29661272, Free PMC Article

    See all (61) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Single-particle cryo-electron microscopy shows that the ExbB pentamer encloses a dimer of ExbD in its transmembrane pore, and not a monomer as previously reported. Ton subcomplex consists of a pentamer of ExbB enclosing a dimer of ExbD TM domains. The essential Asp25 residues on ExbD are in close proximity to the conserved Thr 148 and 181 on ExbB.
      Title: Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry.
    2. Channel current measurement and 2D crystallography support the existence and transition of the two oligomeric states in membranes. The hexameric complex consists of six ExbB subunits and three ExbD transmembrane helices enclosed within the central channel.
      Title: Hexameric and pentameric complexes of the ExbBD energizer in the Ton system.
    3. ExbB-ExbD-TonB form complex in molar ratios of 4:1:1.
      Title: Membrane Protein Complex ExbB4-ExbD1-TonB1 from Escherichia coli Demonstrates Conformational Plasticity.
    4. A 10-residue-deletion scanning mutagenesis indicated that the cytoplasmic loop of ExbB (residues 40 to 129) was essential for TonB system activity.
      Title: ExbB cytoplasmic loop deletions cause immediate, proton motive force-independent growth arrest.
    5. Consideration of combined data excludes ExbB transmembrane domain residues from direct participation in a proton pathway.
      Title: Mutations in Escherichia coli ExbB transmembrane domains identify scaffolding and signal transduction functions and exclude participation in a proton pathway.
    6. ExbB(6) and ExbB(6)ExbD(1) are subcomplexes on which the final complex including TonB is assembled
      Title: Oligomeric structure of ExbB and ExbB-ExbD isolated from Escherichia coli as revealed by LILBID mass spectrometry.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables energy transducer activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in bacteriocin transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cobalamin transport NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within ferrichrome import into cell EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in intracellular iron ion homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in intracellular monoatomic cation homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in protein import IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within protein stabilization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell outer membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in plasma membrane RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    part_of plasma membrane protein complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of transmembrane transporter complex NAS
    Non-traceable Author Statement
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Ton complex subunit ExbB

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3151250..3151984 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417479.1 Ton complex subunit ExbB [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417479.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3I2K8, A0A454A7W5, A0A828U0E6, A0AAN3SGF6, A0AAV3H5U1
      Conserved Domains (1) summary
      PRK10414
      Location:1 → 244
      PRK10414; biopolymer transporter ExbB
    Nucleotide Protein
    Heading Accession and Version

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