NBP35 Fe-S cluster-binding ATPase [Saccharomyces cerevisiae S288C] - Gene

Summary

Official Symbol: NBP35
Official Full Name: Fe-S cluster-binding ATPase
Primary source: SGD:S000003059
Locus tag: YGL091C
See related: AllianceGenome:SGD:S000003059; FungiDB:YGL091C; VEuPathDB:YGL091C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 4 iron, 4 sulfur cluster binding activity; ATP hydrolysis activity; and iron ion binding activity. Involved in iron-sulfur cluster assembly and tRNA wobble uridine modification. Located in cytoplasm and nucleus. Part of cytosolic [4Fe-4S] assembly scaffold complex. Orthologous to human NUBP1 (NUBP iron-sulfur cluster assembly factor 1, cytosolic). [provided by Alliance of Genome Resources, Dec 2024]
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Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Go to nucleotide: Graphics FASTA GenBank

Data suggest hat mutation of D109 in Fe-S cluster-binding ATPase Nbp35 (NBP35) prohibits a conformational change that is required for FeS cluster acquisition.
Title: Coupling Nucleotide Binding and Hydrolysis to Iron-Sulfur Cluster Acquisition and Transfer Revealed through Genetic Dissection of the Nbp35 ATPase Site.
The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase.
Title: The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase.
a model in which Cfd1-Nbp35 interaction increases the lability of assembled FeS on the Nbp35 scaffold for transfer to target apo-FeS proteins.
Title: Interaction with Cfd1 increases the kinetic lability of FeS on the Nbp35 scaffold.
a model for the mode of Fe-S cluster coordination within the Cfd1-Nbp35 complex, explaining its specific properties as a Fe-S scaffold device.
Title: A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfd1-Nbp35 complex as a scaffold in iron-sulfur protein maturation.
suggest that the Cfd1-Nbp35 complex functions as a novel scaffold for [Fe-S] cluster assembly in the eukaryotic cytosol
Title: The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol.
Nbp35 NTPases function in Fe/S protein maturation
Title: The eukaryotic P loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
contributes_to ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP-dependent FeS chaperone activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables iron-sulfur cluster binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in iron-sulfur cluster assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in iron-sulfur cluster assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in iron-sulfur cluster assembly	IEA Inferred from Electronic Annotation more info
involved_in iron-sulfur cluster assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA wobble uridine modification	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of cytosolic [4Fe-4S] assembly scaffold complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

NP_011424.3

Essential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases