rnb RNase II [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: rnb
Official Full Name: RNase II
Primary source: ECOCYC:EG11620
Locus tag: b1286
See related: ASAP:ABE-0004320
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1281
Summary: Ribonuclease II (RNase II) is an exonuclease that cleaves RNA from the 3' end to produce ribonucleoside 5'-monophosphates. [More information is available at EcoCyc: EG11620].
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Genomic context

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Sequence:: NC_000913.3 (1346978..1348912, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Reversible acetylation on Lys501 regulates the activity of RNase II.
Title: Reversible acetylation on Lys501 regulates the activity of RNase II.
We determined that the deletion of RNase II significantly affected 187 transcripts, while deletion of RNase R affects 202 transcripts and deletion of PNPase affected 226 transcripts
Title: Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli.
Data show that RNase II is associated with all of the known degradosome proteins.
Title: The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome.
Data indicate that membrane association and self-interactions are both required for the RNase II cellular organization.
Title: Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II.
The authors propose that RNase II degrades mRNA to the downstream border of paused ribosomes, facilitating cleavage of the A-site codon by an unknown RNase.
Title: RNase II is important for A-site mRNA cleavage during ribosome pausing.
The crystal structure of E. coli RNase II reveals an architecture reminiscent of the RNA exosome.
Title: Structural basis for processivity and single-strand specificity of RNase II.
A functionl characterization of three different domains assigned to the Escherichia coli RNase II - two RNA-binding domains at each end of the protein (CSD and S1), and a central RNB catalytic domain - was performed
Title: Characterization of the functional domains of Escherichia coli RNase II.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 3'-5' exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables exonuclease activity	IEA Inferred from Electronic Annotation more info
enables exoribonuclease II activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA catabolic process	IEA Inferred from Electronic Annotation more info
involved_in mRNA catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in mRNA catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within tRNA decay	EXP Inferred from Experiment more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: RNase II

NP_415802.1

EC 3.1.13.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1346978..1348912 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415802.1 RNase II [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415802.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5D8MRN2

Conserved Domains (1) summary

PRK05054
Location:1 → 643

PRK05054; exoribonuclease II; Provisional