U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from Protein

    • Showing Current items.

    IFI16 interferon gamma inducible protein 16 [ Homo sapiens (human) ]

    Gene ID: 3428, updated on 27-Nov-2024

    Summary

    Official Symbol
    IFI16provided by HGNC
    Official Full Name
    interferon gamma inducible protein 16provided by HGNC
    Primary source
    HGNC:HGNC:5395
    See related
    Ensembl:ENSG00000163565 MIM:147586; AllianceGenome:HGNC:5395
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PYHIN2; IFNGIP1
    Summary
    This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
    Expression
    Ubiquitous expression in lymph node (RPKM 45.3), appendix (RPKM 41.3) and 24 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See IFI16 in Genome Data Viewer
    Location:
    1q23.1
    Exon count:
    14
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (158999976..159055151)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (158137044..158192189)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (158969766..159024941)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:158856217-158856716 Neighboring gene pyrin and HIN domain family member 5, pseudogene Neighboring gene H3K27ac hESC enhancer GRCh37_chr1:158879152-158879652 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:158908087-158909286 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:158922455-158923423 Neighboring gene pyrin and HIN domain family member 1 Neighboring gene ReSE screen-validated silencer GRCh37_chr1:158928812-158928990 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1900 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:158975177-158975702 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:158975703-158976226 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1901 Neighboring gene uncharacterized LOC124904432 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1452 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:158984807-158986006 Neighboring gene pyrin domain containing 5 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1902 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1903 Neighboring gene absent in melanoma 2 Neighboring gene RAD1 pseudogene 2 Neighboring gene tRNA-Arg (anticodon TCT) 4-1

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    EBI GWAS Catalog

    Description
    Genome-wide association and population genetic analysis of C-reactive protein in African American and Hispanic American women.
    EBI GWAS Catalog
    GWAS of blood cell traits identifies novel associated loci and epistatic interactions in Caucasian and African-American children.
    EBI GWAS Catalog
    Meta-analysis of genome-wide association studies in celiac disease and rheumatoid arthritis identifies fourteen non-HLA shared loci.
    EBI GWAS Catalog
    Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
    EBI GWAS Catalog

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Nef nef Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Nef may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed
    Pr55(Gag) gag HIV-1 Gag interacts with IfI16 as demonstrated by proximity dependent biotinylation proteomics PubMed
    Tat tat Microarray analysis indicates HIV-1 Tat upregulates the interferon-responsive gene expression of many proteins, including IFI16, in immature dendritic cells, an effect that likely facilitates the expansion of HIV-1 infection PubMed
    Vpr vpr Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Vpr may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed
    Vpu vpu Production of infectious triple deletion vpr/vpu/nef HIV-1 mutant is suppressed, indicating that Vpu may antagonize restriction activity of IFI16 against production of infectious wild-type HIV-1 PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • MGC9466

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA binding HDA PubMed 
    enables double-stranded DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables double-stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables transcription factor binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in activation of cysteine-type endopeptidase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in activation of innate immune response IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in activation of innate immune response IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in autophagy IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cellular response to glucose starvation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in cellular response to interferon-beta IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in cellular response to ionizing radiation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in defense response to virus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in defense response to virus IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in inflammatory response IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in innate immune response IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in intrinsic apoptotic signaling pathway by p53 class mediator IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    acts_upstream_of_or_within intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in monocyte differentiation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in myeloid cell differentiation NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in negative regulation of AIM2 inflammasome complex assembly IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of DNA binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of cysteine-type endopeptidase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of gene expression, epigenetic IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of innate immune response IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of viral genome replication IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of cytokine production TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in positive regulation of interleukin-1 beta production IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of autophagy IEP
    Inferred from Expression Pattern
    more info
    PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
     
    colocalizes_with cytosol IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in membrane HDA PubMed 
    located_in nuclear speck IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in nucleolus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in nucleoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    gamma-interferon-inducible protein 16
    Names
    interferon-gamma induced protein IFI 16
    interferon-inducible myeloid differentiation transcriptional activator

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_029141.2 RefSeqGene

      Range
      14938..60182
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001206567.2NP_001193496.1  gamma-interferon-inducible protein 16 isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) lacks an in-frame exon in the 5' coding region and has an additional in-frame exon in the 3' coding region, compared to variant 2. The additional exon is described as exon 7a in PMID:9718316. The resulting isoform (1) is of the same size but lacks a segment in the N-terminal region and has an additional segment in the C-terminal region, compared to isoform 2. Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.
      Source sequence(s)
      AK296228, BM806453, DA945454, DB273519, M63838
      Consensus CDS
      CCDS58039.1
      Related
      ENSP00000352740.3, ENST00000359709.7
      Conserved Domains (3) summary
      cd08305
      Location:1083
      Pyrin; Pyrin: a protein-protein interaction domain
      pfam02760
      Location:145313
      HIN; HIN-200/IF120x domain
      pfam06583
      Location:343545
      Neogenin_C; Neogenin C-terminus
    2. NM_001364867.2NP_001351796.1  gamma-interferon-inducible protein 16 isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) encodes the longest isoform (3, also known as IFI 16A).
      Source sequence(s)
      AL359753
      Consensus CDS
      CCDS91079.1
      UniProtKB/Swiss-Prot
      A0A346JEQ3, B4DJT8, H3BLV7, Q16666, Q59GX0, Q5T3W7, Q5T3W8, Q5T3X0, Q5T3X1, Q5T3X2, Q8N9E5, Q8NEQ7, Q96AJ5, Q9UH78
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain
    3. NM_001376587.1NP_001363516.1  gamma-interferon-inducible protein 16 isoform 3

      Status: REVIEWED

      Source sequence(s)
      AL359753
      Consensus CDS
      CCDS91079.1
      UniProtKB/Swiss-Prot
      A0A346JEQ3, B4DJT8, H3BLV7, Q16666, Q59GX0, Q5T3W7, Q5T3W8, Q5T3X0, Q5T3X1, Q5T3X2, Q8N9E5, Q8NEQ7, Q96AJ5, Q9UH78
      Related
      ENSP00000295809.7, ENST00000295809.12
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain
    4. NM_001376588.1NP_001363517.1  gamma-interferon-inducible protein 16 isoform 2

      Status: REVIEWED

      Source sequence(s)
      AL359753
      Consensus CDS
      CCDS1180.3
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain
    5. NM_001376589.1NP_001363518.1  gamma-interferon-inducible protein 16 isoform 2

      Status: REVIEWED

      Source sequence(s)
      AL359753
      Consensus CDS
      CCDS1180.3
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain
    6. NM_001376591.1NP_001363520.1  gamma-interferon-inducible protein 16 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AL359753
      Consensus CDS
      CCDS91080.1
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain
    7. NM_001376592.1NP_001363521.1  gamma-interferon-inducible protein 16 isoform 4

      Status: REVIEWED

      Source sequence(s)
      AL359753
      Consensus CDS
      CCDS91080.1
      Related
      ENSP00000404325.2, ENST00000448393.6
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain
    8. NM_005531.3NP_005522.2  gamma-interferon-inducible protein 16 isoform 2

      See identical proteins and their annotated locations for NP_005522.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) consists of 11 exons and encodes isoform 2 (also known as isoform B, PMIDs:9718316 and 12588705). Note the CDS was annotated to initiate translation from an in-frame downstream AUG, as it is flanked by a strong Kozak signal, and is better conserved than the upstream AUG located 4 codons upstream.
      Source sequence(s)
      AL359753, BC017059, DA990524, M63838
      Consensus CDS
      CCDS1180.3
      Related
      ENSP00000357113.4, ENST00000368131.8
      Conserved Domains (3) summary
      PTZ00449
      Location:100215
      PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
      cd08305
      Location:1083
      Pyrin; a protein-protein interaction domain
      pfam02760
      Location:201369
      HIN; HIN-200/IF120x domain

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      158999976..159055151
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047419411.1XP_047275367.1  gamma-interferon-inducible protein 16 isoform X2

    2. XM_047419406.1XP_047275362.1  gamma-interferon-inducible protein 16 isoform X1

    3. XM_047419399.1XP_047275355.1  gamma-interferon-inducible protein 16 isoform X1

    4. XM_047419413.1XP_047275369.1  gamma-interferon-inducible protein 16 isoform X2

    RNA

    1. XR_007059344.1 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      158137044..158192189
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054336307.1XP_054192282.1  gamma-interferon-inducible protein 16 isoform X1

    2. XM_054336310.1XP_054192285.1  gamma-interferon-inducible protein 16 isoform X2

    3. XM_054336308.1XP_054192283.1  gamma-interferon-inducible protein 16 isoform X1

    4. XM_054336306.1XP_054192281.1  gamma-interferon-inducible protein 16 isoform X1

    5. XM_054336309.1XP_054192284.1  gamma-interferon-inducible protein 16 isoform X2

    RNA

    1. XR_008486021.1 RNA Sequence

    2. XR_008486020.1 RNA Sequence