Indy I'm not dead yet [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Indyprovided by FlyBase
Official Full Name: I'm not dead yetprovided by FlyBase
Primary source: FLYBASE:FBgn0036816
Locus tag: Dmel_CG3979
See related: AllianceGenome:FB:FBgn0036816
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-EST:fe3A7; anon-WO0172774.70; BEST:LP01220; CG3979; Dmel\CG3979; drIndy; indy; INDY
Summary: Enables citrate transmembrane transporter activity; pyruvate transmembrane transporter activity; and succinate transmembrane transporter activity. Involved in carboxylic acid transport; determination of adult lifespan; and regulation of sequestering of triglyceride. Located in plasma membrane. Is expressed in several structures, including adult antennal lobe; adult brain; adult brain cell body rind; adult brain surface glial cell; and embryonic/larval midgut primordium. Used to study Kohlschutter-Tonz syndrome; alcohol-related neurodevelopmental disorder; and epilepsy. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy 25. Orthologous to several human genes including SLC13A2 (solute carrier family 13 member 2); SLC13A3 (solute carrier family 13 member 3); and SLC13A5 (solute carrier family 13 member 5). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 75E1-75E2; 3-45 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (18828632..18846267, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (18821732..18839367, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our data suggest INDY may be a physiological regulator that modulates intermediary metabolism in response to changes in nutrient availability and organismal needs by modulating dPGC-1.
Title: Increased mitochondrial biogenesis preserves intestinal stem cell homeostasis and contributes to longevity in Indy mutant flies.
Evolution reaffirms that the mechanism of heterozygote advantage has acted upon the Indy gene to assure increased reproductive fitness and, coincidentally, longer life span through regulatory transposon mutagenesis.
Title: Indy gene variation in natural populations confers fitness advantage and life span extension through transposon insertion.
Study concludes that Indy and calorie restriction (CR) interact to affect longevity and that a decrease in Indy may induce a CR-like status that confers life span extension.
Title: Long-lived Indy and calorie restriction interact to extend life span.
Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage
Title: Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage.
Does not influence lifespan in Drosophila after correction for genetic and cytoplasmic background effects.
Title: No influence of Indy on lifespan in Drosophila after correction for genetic and cytoplasmic background effects.
the transporter encoded by the life-extending gene Indy is a disulphonic stilbene-sensitive, sodium-independent anion exchanger that is capable of exchanging pairs of dicarboxylates and citrate across the plasma membrane
Title: The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates.
Indy mutation causes a decrease in the slope of the mortality curve consistent with a slowing in the rate of aging without a concomitant reduction in resting metabolic rate, flight velocity, or age-specific fecundity.
Title: Conditional tradeoffs between aging and organismal performance of Indy long-lived mutant flies.
drIndy is a cation-independent electroneutral transporter for a variety of tricarboxylic acid-cycle intermediates, with preference for citrate compared with succinate.
Title: Functional identity of Drosophila melanogaster Indy as a cation-independent, electroneutral transporter for tricarboxylic acid-cycle intermediates.
INDY functions as a novel sodium-independent mechanism for transporting Krebs and citric acid cycle intermediates through the epithelium of the gut and across the plasma membranes of organs involved in intermediary metabolism and storage
Title: Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm2065 (e-PCR)
- UniSTS:43260

Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: c18834940-18833837 mobile_element_type = transposon:1360{}1044

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables citrate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables citrate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables pyruvate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables succinate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables succinate transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in citrate transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in citrate transport	IDA Inferred from Direct Assay more info	PubMed
involved_in determination of adult lifespan	IDA Inferred from Direct Assay more info	PubMed
NOT involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pyruvate transport	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of sequestering of triglyceride	IDA Inferred from Direct Assay more info	PubMed
involved_in succinate transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in succinate transport	IDA Inferred from Direct Assay more info	PubMed
involved_in transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: I'm not dead yet

Names: CG3979-PA; CG3979-PB; CG3979-PC; CG3979-PD; I am not dead yet; I'm Not dead Yet; Indy-PA; Indy-PB; Indy-PC; Indy-PD; anon-fast-evolving-3A7

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

18828632..18846267 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_168778.2 → NP_730363.2 I'm not dead yet, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730363.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4V236, Q7KUS6, Q9NHY9, Q9VVT2

UniProtKB/TrEMBL

D0IQJ2

Related

FBpp0074848

Conserved Domains (2) summary

cd01115
Location:68 → 545

SLC13_permease; Permease SLC13 (solute carrier 13). The sodium/dicarboxylate cotransporter NaDC-1 has been shown to translocate Krebs cycle intermediates such as succinate, citrate, and alpha-ketoglutarate across plasma membranes rabbit, human, and rat kidney. It is ...

TIGR00785
Location:70 → 545

dass; anion transporter
NM_168779.2 → NP_730364.1 I'm not dead yet, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730364.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JI19

Related

FBpp0074849

Conserved Domains (2) summary

cd01115
Location:50 → 527

SLC13_permease; Permease SLC13 (solute carrier 13). The sodium/dicarboxylate cotransporter NaDC-1 has been shown to translocate Krebs cycle intermediates such as succinate, citrate, and alpha-ketoglutarate across plasma membranes rabbit, human, and rat kidney. It is ...

TIGR00785
Location:52 → 527

dass; anion transporter
NM_079426.4 → NP_524150.1 I'm not dead yet, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524150.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JI19

Related

FBpp0074850

Conserved Domains (2) summary

cd01115
Location:50 → 527

SLC13_permease; Permease SLC13 (solute carrier 13). The sodium/dicarboxylate cotransporter NaDC-1 has been shown to translocate Krebs cycle intermediates such as succinate, citrate, and alpha-ketoglutarate across plasma membranes rabbit, human, and rat kidney. It is ...

TIGR00785
Location:52 → 527

dass; anion transporter
NM_001169994.2 → NP_001163465.1 I'm not dead yet, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163465.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JI19

Conserved Domains (2) summary

cd01115
Location:50 → 527

SLC13_permease; Permease SLC13 (solute carrier 13). The sodium/dicarboxylate cotransporter NaDC-1 has been shown to translocate Krebs cycle intermediates such as succinate, citrate, and alpha-ketoglutarate across plasma membranes rabbit, human, and rat kidney. It is ...

TIGR00785
Location:52 → 527

dass; anion transporter