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    MUS81 Mus81p [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851994, updated on 9-Dec-2024

    Summary

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    Official Symbol
    MUS81
    Official Full Name
    Mus81p
    Primary source
    SGD:S000002794
    Locus tag
    YDR386W
    See related
    AllianceGenome:SGD:S000002794; FungiDB:YDR386W; VEuPathDB:YDR386W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    SLX3
    Summary
    Enables enzyme inhibitor activity. Contributes to crossover junction DNA endonuclease activity. Involved in DNA topological change; double-strand break repair via break-induced replication; and resolution of meiotic recombination intermediates. Located in nucleus. Part of Holliday junction resolvase complex. Orthologous to human MUS81 (MUS81 structure-specific endonuclease subunit). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See MUS81 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (1246084..1247982)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene putative ammonium permease ATO3 Neighboring gene elongation factor 2 Neighboring gene Cin10p Neighboring gene amphiphysin

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Cleavage of model replication forks by fission yeast Mus81-Eme1 and budding yeast Mus81-Mms4. Whitby MC, et al. J Biol Chem, 2003 Feb 28. PMID 12473680
    2. MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Interthal H, et al. Mol Gen Genet, 2000 Jun. PMID 10905349
    3. The possible function of Flp1 in homologous recombination repair in Saccharomyces cerevisiae. Phung HTT, et al. AIMS Genet, 2018. PMID 31435519, Free PMC Article
    4. The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage. Mukherjee S, et al. Nucleic Acids Res, 2014 Jun. PMID 24744239, Free PMC Article
    5. Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. Fu Y, et al. DNA Repair (Amst), 2003 Dec 9. PMID 14642571

    See all (170) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors.
      Title: Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursors.
    2. MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.
      Title: MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.
    3. The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
      Title: The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
    4. Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
      Title: Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
    5. Study presents evidence that yeast Mus81-Mms4 endonuclease contributes to cell viability in the absence of functional telomerase. This involvement is not through recombination-mediated telomere lengthening as proposed in humans, but rather through an indirect role involving replication stress. These and other results suggest that Mus81 localization to the telomere may be a promising indicator for telomere dysfunction.
      Title: Saccharomyces cerevisiae Mus81-Mms4 prevents accelerated senescence in telomerase-deficient cells.
    6. These results strongly support the notion that Rad52 and Mus81-Mms4 collaborate and work jointly in processing of homologous recombination intermediates.
      Title: Saccharomyces cerevisiae Mus81-Mms4 and Rad52 can cooperate in the resolution of recombination intermediates.
    7. Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
      Title: Role of PCNA and RFC in promoting Mus81-complex activity.
    8. The data suggest that subnuclear relocalization is relevant for the function of Mus81-Mms4 complex in response to DNA damage and, probably, of the endonucleases that colocalize with it.
      Title: Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage.
    9. The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation.
      Title: Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis.
    10. The data point to the possibility that the involvement of Esc2 in the metabolism of sister chromatid junctions is, in part, via modulation of the activity of the Mus81 complex.
      Title: Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains.
    Submit: New GeneRIF Correction See all GeneRIFs (31)

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables 3'-flap endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to DNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    contributes_to crossover junction DNA endonuclease activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    contributes_to crossover junction DNA endonuclease activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables crossover junction DNA endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables endonuclease activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme inhibitor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA damage response IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA topological change IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in double-strand break repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in double-strand break repair via break-induced replication IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in double-strand break repair via break-induced replication IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in double-strand break repair via break-induced replication IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiosis I cell cycle process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in meiotic cell cycle IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in mitotic intra-S DNA damage checkpoint signaling IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in reciprocal meiotic recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in resolution of meiotic recombination intermediates IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in resolution of meiotic recombination intermediates IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of Holliday junction resolvase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of Holliday junction resolvase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    Mus81p
    NP_010674.3
    • Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA and DNA-protein cross-links; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates function of Mms4p-Mus81p

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      1246084..1247982
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180694.3 → NP_010674.3  TPA: Mus81p [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010674.3

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VT20, Q04149
      UniProtKB/TrEMBL
      A6ZYY7, B3LFR4, C7GSD6, C8Z5V0, N1P8S9
      Conserved Domains (2) summary
      COG1948
      Location:314 → 601
      MUS81; ERCC4-type nuclease [Replication, recombination and repair]
      cd21036
      Location:132 → 224
      WH_MUS81; winged helix domain found in crossover junction endonuclease MUS81 and similar proteins
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q04149.1

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