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    prfC peptide chain release factor RF3 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948897, updated on 3-Dec-2024

    Summary

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    Official Symbol
    prfC
    Official Full Name
    peptide chain release factor RF3
    Primary source
    ECOCYC:EG12114
    Locus tag
    b4375
    See related
    ASAP:ABE-0014346
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK4366; miaD; tos
    Summary
    RF3 exits in two conformational states, state 1 which is a pre-translocation complex with a P-site tRNA and a pre-bound RF3 (inactive) while state 2 is a post-translocation conformation with RF3 tightly bound to the GTPase centre and the tRNA at the exit site. [More information is available at EcoGene: EG12114]. Release factor 3 (RF3) is a ribosome-dependent GTPase that stimulates the release of RF1 and RF2 from the ribosome after peptide chain termination. [More information is available at EcoCyc: EG12114].
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    Genomic context

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    Sequence:
    NC_000913.3 (4609414..4611003)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA polymerase III subunit psi Neighboring gene protein N-acetyltransferase RimI Neighboring gene pyrimidine 5'-nucleotidase YjjG Neighboring gene periplasmic chaperone OsmY Neighboring gene DUF1328 domain-containing protein YtjA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A primary role for release factor 3 in quality control during translation elongation in Escherichia coli. Zaher HS, et al. Cell, 2011 Oct 14. PMID 22000017, Free PMC Article
    2. Release factor RF-3 GTPase activity acts in disassembly of the ribosome termination complex. Grentzmann G, et al. RNA, 1998 Aug. PMID 9701288, Free PMC Article
    3. Osmo-expression and fast two-step purification of Escherichia coli translation termination factor RF-3. Mortensen KK, et al. Eur J Biochem, 1995 Dec 15. PMID 8575429
    4. Localization and characterization of the gene encoding release factor RF3 in Escherichia coli. Grentzmann G, et al. Proc Natl Acad Sci U S A, 1994 Jun 21. PMID 8016077, Free PMC Article
    5. Identification of the prfC gene, which encodes peptide-chain-release factor 3 of Escherichia coli. Mikuni O, et al. Proc Natl Acad Sci U S A, 1994 Jun 21. PMID 8016068, Free PMC Article

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Ribosome termination complexes that carry two factors, RF1-RF3 or RF2-RF3, are dynamic and fluctuate between non-rotated and rotated states, whereas each factor alone has its distinct signature on ribosome dynamics and conformation.
      Title: Dynamics of ribosomes and release factors during translation termination in E. coli.
    2. Data show that peptide chain release factor RF-3 (RF3) hydrolyzes GTP and dissociates from the ribosome before peptide chain release factor RF-1 (RF1)dissociation.
      Title: Mechanism of Translation Termination: RF1 Dissociation Follows Dissociation of RF3 from the Ribosome.
    3. Results indicate that peptide chain release factor RF3 loss is well tolerated in bacteria with fully active class I release factors.
      Title: Interactions of release factor RF3 with the translation machinery.
    4. Together, these data define a more precise role for RF3 in translation termination and provide insights into the function of this family of translational GTPases
      Title: RF3:GTP promotes rapid dissociation of the class 1 termination factor.
    5. Ordering of critical elements of switch loop I and the P loop, which help to form the GTPase catalytic site, are caused by interactions between the G domain of RF3.
      Title: Crystal structure of release factor RF3 trapped in the GTP state on a rotated conformation of the ribosome.
    6. Data suggest how RF3, by stabilizing the hybrid state of the ribosome, facilitates the dissociation of class I release factors.
      Title: Crystal structure of the hybrid state of ribosome in complex with the guanosine triphosphatase release factor 3.
    7. Study examines the role of RF3 in the bacterial cell and show that the deletion of this gene sensitizes cells to other perturbations that reduce the overall fidelity of protein synthesis.
      Title: A primary role for release factor 3 in quality control during translation elongation in Escherichia coli.
    8. Data show a novel class of RF3 mutations specifically defective in the tRNA drop-off reaction, and these mutations suggest differential molecular pathways closely related to the guanine nucleotide modes of RF3.
      Title: A novel class of bacterial translation factor RF3 mutations suggests specific structural domains for premature peptidyl-tRNA drop-off.
    9. N-terminus verified by Edman degradation on mature peptide
      Title: Osmo-expression and fast two-step purification of Escherichia coli translation termination factor RF-3.
    10. The data show that RF3*GTP binding induces large conformational changes in the ribosome, which break the interactions of the class I RF with both the decoding center and the GTPase-associated center leading to the release of the class I RF.
      Title: RF3 induces ribosomal conformational changes responsible for dissociation of class I release factors.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables GDP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanosine tetraphosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables translation release factor activity, codon nonspecific IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables translation release factor activity, codon nonspecific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation release factor activity, codon specific IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in maintenance of translational fidelity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in regulation of translational termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational termination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within translational termination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translational termination IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    peptide chain release factor RF3

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4609414..4611003
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418792.1 peptide chain release factor RF3 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418792.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MEK9, A0A8S7CUK5
      Conserved Domains (1) summary
      PRK00741
      Location:4 → 529
      prfC; peptide chain release factor 3; Provisional
    Nucleotide Protein
    Heading Accession and Version

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