chek1.S checkpoint kinase 1 S homeolog [Xenopus laevis (African clawed frog)] - Gene

Summary

Official Symbol: chek1.S
Official Full Name: checkpoint kinase 1 S homeolog
Primary source: Xenbase:XB-GENE-866102
Locus tag: XELAEV_18037351mg
See related: EnsemblRapid:ENSXLAG00005022536 AllianceGenome:Xenbase:XB-GENE-866102
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Xenopus laevis
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus
Also known as: chk1; chek1
Summary: Predicted to enable histone H3T11 kinase activity. Acts upstream of or within protein phosphorylation. Predicted to be located in centrosome; chromatin; and condensed nuclear chromosome. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human CHEK1 (checkpoint kinase 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

Location:: chromosome: 7S

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
101	current	Xenopus_laevis_v10.1 (GCF_017654675.1)	7S	NC_054384.1 (61934586..61959784, complement)
100	previous assembly	Xenopus_laevis_v2 (GCF_001663975.1)	7S	NC_030737.1 (48799755..48826045, complement)

Chromosome 7S - NC_054384.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inhibition of Drf1 is the primary mechanism by which Chk1 blocks cell-cycle progression in the early embryo and is an essential function of Chk1 at the blastula-to-gastrula stage of development.
Title: Chk1 Inhibition of the Replication Factor Drf1 Guarantees Cell-Cycle Elongation at the Xenopus laevis Mid-blastula Transition.
The study analyzes the role of Chk1 in the replication program in sperm nuclei replicating in Xenopus egg extracts by a combination of experimental and modelling approaches.
Title: Tight Chk1 Levels Control Replication Cluster Activation in Xenopus.
ATR-Chk1 DDR pathway appears to be dispensable for the preferential association of REV1 to MMC-damaged chromatin.
Title: REV1 is important for the ATR-Chk1 DNA damage response pathway in Xenopus egg extracts.
Results show that Chk1 negatively regulates the Treslin-mediated loading of Cdc45 onto chromatin and thereby serves to antagonize the initiation of replication.
Title: Interaction of Chk1 with Treslin negatively regulates the initiation of chromosomal DNA replication.
The MRN complex, in particular the nuclease activity of Mre11, plays an important role in the activation of Chk1 in response to stalled replication forks.
Title: The Mre11-Rad50-Nbs1 (MRN) complex has a specific role in the activation of Chk1 in response to stalled replication forks.
DNA polymerase kappa-dependent DNA synthesis at stalled replication forks is important for CHK1 activation.
Title: DNA polymerase κ-dependent DNA synthesis at stalled replication forks is important for CHK1 activation.
APE2 associates with Chk1; a serine residue (S86) in the Chk1-binding motif of APE2 is essential for Chk1 phosphorylation, indicating a Claspin-like but distinct role for APE2 in ATR-Chk1 signaling.
Title: APE2 is required for ATR-Chk1 checkpoint activation in response to oxidative stress.
Data show that the death pathway is independent of ERK but relies on activating Bad phosphorylation through the control of both kinases Cdk1 and JNK.
Title: Unfertilized Xenopus eggs die by Bad-dependent apoptosis under the control of Cdk1 and JNK.
Findings reveal that XH2AX has a specific role in anterior neural formation of Xenopus, which is mediated through phosphorylation of XH2AX at Thr(16) by Chk1.
Title: Histone XH2AX is required for Xenopus anterior neural development: critical role of threonine 16 phosphorylation.
Chk1 is activated at the midblastula transition in Xenopus laevis embryos independently of DNA content and the cyclin E/Cdk2 developmental timer.
Title: Chk1 is activated at the midblastula transition in Xenopus laevis embryos independently of DNA content and the cyclin E/Cdk2 developmental timer.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by Xenbase

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage checkpoint signaling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA damage checkpoint signaling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA damage response	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within chromatin remodeling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within mitotic G2 DNA damage checkpoint signaling	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of gene expression, epigenetic	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of mitotic nuclear division	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within peptidyl-threonine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of double-strand break repair via homologous recombination	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of mitotic centrosome separation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within signal transduction in response to DNA damage	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatin	ISS Inferred from Sequence or Structural Similarity more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in condensed nuclear chromosome	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: serine/threonine-protein kinase Chk1

Names: CHK1 checkpoint homolog; Chk1 checkpoint kinase; checkpoint kinase-1; xChk1

NP_001082039.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001088570.1 → NP_001082039.1 serine/threonine-protein kinase Chk1

See identical proteins and their annotated locations for NP_001082039.1

Status: PROVISIONAL

Source sequence(s)

AF053120

UniProtKB/Swiss-Prot

Q6DE87, Q78CK1, Q78DQ2, Q9YI18

UniProtKB/TrEMBL

A0A974CC65

Related

ENSXLAP00005066481.1, ENSXLAT00005068005.1

Conserved Domains (2) summary

smart00220
Location:9 → 265

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd14069
Location:7 → 265

STKc_Chk1; Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1

RefSeqs of Annotated Genomes: Xenopus laevis Annotation Release 101 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference Xenopus_laevis_v10.1 Primary Assembly

Genomic

NC_054384.1 Reference Xenopus_laevis_v10.1 Primary Assembly

Range

61934586..61959784 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)