Rnaseh2a ribonuclease H2, large subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rnaseh2aprovided by MGI
Official Full Name: ribonuclease H2, large subunitprovided by MGI
Primary source: MGI:MGI:1916974
See related: Ensembl:ENSMUSG00000052926 AllianceGenome:MGI:1916974
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: RNHL; RNHIA; RNASEHI; 2400006P09Rik
Summary: Predicted to enable RNA-DNA hybrid ribonuclease activity. Acts upstream of or within mismatch repair. Part of ribonuclease H2 complex. Is expressed in several structures, including embryo ectoderm; gut; hemolymphoid system gland; skin; and testis. Human ortholog(s) of this gene implicated in Aicardi-Goutieres syndrome. Orthologous to human RNASEH2A (ribonuclease H2 subunit A). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in testis adult (RPKM 196.9), liver E14 (RPKM 12.9) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 C3; 8 41.38 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (85683239..85694498, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (84956610..84969598, complement)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells.
Title: RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells.
An RNase H2 mutant lacking ribonucleotide excision repair activity but supporting R-loop removal revealed that ribonucleotides in DNA initiate p53-dependent DNA damage response and early embryonic arrest in mouse.
Title: Two RNase H2 Mutants with Differential rNMP Processing Activity Reveal a Threshold of Ribonucleotide Tolerance for Embryonic Development.
the G37S mutation of RNaseH2 led to increased expression of interferon-stimulated genes dependent on the cGAS-STING signaling pathway.
Title: RNase H2 catalytic core Aicardi-Goutières syndrome-related mutant invokes cGAS-STING innate immune-sensing pathway in mice.
Recent evidence gathered from ribonuclease H2 knock-out mice provides insight into the molecular mechanisms underlying Aicardi-Goutieres syndrome development and a potential role of DNA damage as a trigger of autoimmunity is discussed. [review]
Title: Aicardi-Goutières syndrome: clues from the RNase H2 knock-out mouse.
analysis of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome
Title: Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome.
Data show that the interwoven architecture of RNase H2B and RNase H2C that interface with RNase H2A in a complex ideally suited for nucleic acid binding and hydrolysis coupled to protein-protein interaction motifs.
Title: The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Gene trapped (1)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA-DNA hybrid ribonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA-DNA hybrid ribonuclease activity	ISO Inferred from Sequence Orthology more info
enables RNA-DNA hybrid ribonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA replication, removal of RNA primer	IBA Inferred from Biological aspect of Ancestor more info
involved_in RNA catabolic process	ISO Inferred from Sequence Orthology more info
involved_in RNA catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in mismatch repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within mismatch repair	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mismatch repair	ISO Inferred from Sequence Orthology more info	PubMed
involved_in mismatch repair	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
part_of ribonuclease H2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonuclease H2 complex	IDA Inferred from Direct Assay more info	PubMed
part_of ribonuclease H2 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of ribonuclease H2 complex	ISO Inferred from Sequence Orthology more info
part_of ribonuclease H2 complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: ribonuclease H2 subunit A

Names: RNase H2 subunit A; RNase HI large subunit; ribonuclease HI large subunit; ribonuclease HI subunit A

NP_001351299.1

EC 3.1.26.4

NP_081463.1

EC 3.1.26.4

XP_011246799.1

EC 3.1.26.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001364370.2 → NP_001351299.1 ribonuclease H2 subunit A isoform 2

Status: VALIDATED

Source sequence(s)

AC163703

Consensus CDS

CCDS90429.1

UniProtKB/TrEMBL

F6RP29, Q05C51

Related

ENSMUSP00000066769.9, ENSMUST00000065049.15

Conserved Domains (1) summary

cd07181
Location:31 → 251

RNase_HII_eukaryota_like; Eukaryotic RNase HII
NM_027187.5 → NP_081463.1 ribonuclease H2 subunit A isoform 1

See identical proteins and their annotated locations for NP_081463.1

Status: VALIDATED

Source sequence(s)

AC163703

Consensus CDS

CCDS22486.1

UniProtKB/Swiss-Prot

Q8CHY8, Q9CWY8

UniProtKB/TrEMBL

F6RP29

Related

ENSMUSP00000105360.4, ENSMUST00000109738.10

Conserved Domains (1) summary

cd07181
Location:31 → 251

RNase_HII_eukaryota_like; Eukaryotic RNase HII