Prkag3 protein kinase, AMP-activated, gamma 3 non-catalytic subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkag3provided by MGI
Official Full Name: protein kinase, AMP-activated, gamma 3 non-catalytic subunitprovided by MGI
Primary source: MGI:MGI:1891343
See related: Ensembl:ENSMUSG00000006542 AllianceGenome:MGI:1891343
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Amkg3; Ampkg3; AMPKg3L; AMPKg3S
Summary: Contributes to AMP-activated protein kinase activity. Acts upstream of or within negative regulation of glycogen biosynthetic process; regulation of glycolytic process; and response to muscle activity involved in regulation of muscle adaptation. Predicted to be located in cytosol and nucleoplasm. Predicted to be part of nucleotide-activated protein kinase complex. Predicted to be active in cytoplasm and nucleus. Is expressed in several structures, including heart and pericardium and nerve. Orthologous to human PRKAG3 (protein kinase AMP-activated non-catalytic subunit gamma 3). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in mammary gland adult (RPKM 4.6), lung adult (RPKM 3.6) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 C4; 1 38.54 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (74778062..74788623, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (74738922..74749471, complement)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The human AMPKgamma3 R225W mutation negatively impacts site-1 nucleotide binding and does not enhance basal AMPKgamma3-associated activity nor glycogen production in human or mouse skeletal muscle.
Title: The human AMPKγ3 R225W mutation negatively impacts site-1 nucleotide binding and does not enhance basal AMPKγ3-associated activity nor glycogen production in human or mouse skeletal muscle.
AMPKgamma3 Controls Muscle Glucose Uptake in Recovery From Exercise to Recapture Energy Stores.
Title: AMPKγ3 Controls Muscle Glucose Uptake in Recovery From Exercise to Recapture Energy Stores.
Compound- and fiber type-selective requirement of AMPKgamma3 for insulin-independent glucose uptake in skeletal muscle.
Title: Compound- and fiber type-selective requirement of AMPKγ3 for insulin-independent glucose uptake in skeletal muscle.
Data suggest that the AMPK-TBC1D4 signaling axis is likely mediating the improved muscle insulin sensitivity after contraction/exercise and illuminates an important and physiologically relevant role of AMPK in skeletal muscle.
Title: Enhanced Muscle Insulin Sensitivity After Contraction/Exercise Is Mediated by AMPK.
The AMPKgamma3 isoform is dispensable for functional overload-induced muscle growth.
Title: AMPKγ3 is dispensable for skeletal muscle hypertrophy induced by functional overload.
gamma3-AMPK down-regulation is a rapid physiological muscle response observed in mouse after running exercise or fasting, two situations leading to PPARbeta activation.
Title: PPARbeta activation induces rapid changes of both AMPK subunit expression and AMPK activation in mouse skeletal muscle.
AMPK acts as the primordial trigger for fasting- and exercise-induced adaptations in skeletal muscle and that activation of SIRT1 and its downstream signaling pathways are improperly triggered in AMPK-deficient states.
Title: Interdependence of AMPK and SIRT1 for metabolic adaptation to fasting and exercise in skeletal muscle.
subunit R225Q mutation effect on growth performance with normal or high-energy diet
Title: Mouse AMP-activated protein kinase gamma3 subunit R225Q mutation affecting mouse growth performance when fed a high-energy diet.
Basal and A-769662-mediated IL-6 release provide evidence for a role of AMPK in the regulation of IL-6 release from oxidative skeletal muscle.
Title: Role of adenosine 5'-monophosphate-activated protein kinase in interleukin-6 release from isolated mouse skeletal muscle.
AMPK gamma3 subunit is associated with mitochondrial biogenesis in glycolytic skeletal muscle, concomitant with increased expression of the co-activator PGC-1alpha and several transcription factors that regulate mitochondrial proteins
Title: Gain-of-function R225Q mutation in AMP-activated protein kinase gamma3 subunit increases mitochondrial biogenesis in glycolytic skeletal muscle.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)
Targeted (4) 1 citation

General gene information

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Markers

NoName (e-PCR)
- UniSTS:492310

G67375 (e-PCR)
- UniSTS:186393

NoName (e-PCR)
- UniSTS:548505

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables AMP binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to AMP-activated protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to AMP-activated protein kinase activity	ISO Inferred from Sequence Orthology more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein kinase regulator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables protein kinase regulator activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in cellular response to glucose starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to nutrient levels	ISO Inferred from Sequence Orthology more info
involved_in fatty acid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of glycogen biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of carbon utilization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of glycolytic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to muscle activity involved in regulation of muscle adaptation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in nucleoplasm	TAS Traceable Author Statement more info
part_of nucleotide-activated protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of nucleotide-activated protein kinase complex	ISO Inferred from Sequence Orthology more info
part_of nucleotide-activated protein kinase complex	ISS Inferred from Sequence or Structural Similarity more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: 5'-AMP-activated protein kinase subunit gamma-3

Names: AMP-activated protein kinase gamma subunit; AMPK gamma3; AMPK subunit gamma-3; protein kinase, AMP-activated, gamma 3 non-catatlytic subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_153744.4 → NP_714966.1 5'-AMP-activated protein kinase subunit gamma-3

See identical proteins and their annotated locations for NP_714966.1

Status: VALIDATED

Source sequence(s)

AC152409

Consensus CDS

CCDS15055.1

UniProtKB/Swiss-Prot

Q0VG42, Q80WK8, Q8BGM7, Q8CJ41

Related

ENSMUSP00000125344.2, ENSMUST00000160732.8

Conserved Domains (2) summary

cd04618
Location:192 → 329

CBS_euAMPK_gamma-like_repeat1; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains found in AMP-activated protein kinase gamma-like proteins, repeat 1

cd04641
Location:355 → 478

CBS_euAMPK_gamma-like_repeat2; CBS pair domain found in 5'-AMP (adenosine monophosphate)-activated protein kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

74778062..74788623 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006495988.5 → XP_006496051.1 5'-AMP-activated protein kinase subunit gamma-3 isoform X1

See identical proteins and their annotated locations for XP_006496051.1

UniProtKB/Swiss-Prot

Q0VG42, Q80WK8, Q8BGM7, Q8CJ41

Conserved Domains (2) summary

cd04618
Location:192 → 329

CBS_euAMPK_gamma-like_repeat1; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains found in AMP-activated protein kinase gamma-like proteins, repeat 1

cd04641
Location:355 → 478

CBS_euAMPK_gamma-like_repeat2; CBS pair domain found in 5'-AMP (adenosine monophosphate)-activated protein kinase
XM_006495989.5 → XP_006496052.1 5'-AMP-activated protein kinase subunit gamma-3 isoform X1

See identical proteins and their annotated locations for XP_006496052.1

UniProtKB/Swiss-Prot

Q0VG42, Q80WK8, Q8BGM7, Q8CJ41

Conserved Domains (2) summary

cd04618
Location:192 → 329

CBS_euAMPK_gamma-like_repeat1; Two tandem repeats of the cystathionine beta-synthase (CBS pair) domains found in AMP-activated protein kinase gamma-like proteins, repeat 1

cd04641
Location:355 → 478

CBS_euAMPK_gamma-like_repeat2; CBS pair domain found in 5'-AMP (adenosine monophosphate)-activated protein kinase

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_153745.3: Suppressed sequence

Description

NM_153745.3: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.