| Co-invalidation of Prnp and Sprn in FVB/N mice affects reproductive performances and highlight complex biological relationship between PrP and Shadoo. | Co-invalidation of Prnp and Sprn in FVB/N mice affects reproductive performances and highlight complex biological relationship between PrP and Shadoo.
Castille J, Passet B, Makhzami S, Vilotte M, Moazami-Goudarzi K, Truchet S, Daniel-Carlier N, Gaillard AL, Andréoletti O, Vaiman D, Beauvallet C, Vaiman A, Floriot S, Calvel P, Mouillet-Richard S, Duchesne A, Béringue V, Vilotte JL. | 06/12/2021 |
| The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation. | The Prion-like protein Shadoo is involved in mouse embryonic and mammary development and differentiation.
Passet B, Castille J, Makhzami S, Truchet S, Vaiman A, Floriot S, Moazami-Goudarzi K, Vilotte M, Gaillard AL, Helary L, Bertaud M, Andréoletti O, Vaiman D, Calvel P, Daniel-Carlier N, Moudjou M, Beauvallet C, Benharouga M, Laloé D, Mouillet-Richard S, Duchesne A, Béringue V, Vilotte JL., Free PMC Article | 12/12/2020 |
| These data are indicative of a physiological pathway to access Sho functions in the nucleus under conditions of impaired proteasomal activity. | Proteasomal Inhibition Redirects the PrP-Like Shadoo Protein to the Nucleus.
Kang SG, Mays CE, Daude N, Yang J, Kar S, Westaway D., Free PMC Article | 03/14/2020 |
| Data suggest that expression of shadoo in neuron or hepatocyte cell lines induces same toxic phenotype of drug hypersensitivity as prion protein/PrP(-central region); this effect is counteracted by co-expression of PrP-wild-type. | Expression of the Prion Protein Family Member Shadoo Causes Drug Hypersensitivity That Is Diminished by the Coexpression of the Wild Type Prion Protein.
Nyeste A, Bencsura P, Vida I, Hegyi Z, Homolya L, Fodor E, Welker E., Free PMC Article | 08/6/2016 |
| No significant differences in survival, the incubation period of prion disease or other disease features were observed between Sho mutant and WT mice | Unchanged survival rates of Shadoo knockout mice after infection with mouse-adapted scrapie.
Li S, Ju C, Han C, Li Z, Liu W, Ye X, Xu J, Xulong L, Wang X, Chen Z, Meng K, Wan J., Free PMC Article | 09/26/2015 |
| These observations suggest that Shadoo can affect the prion replication process by (i) acting as a holdase and (ii) interfering with the dominant-negative inhibitor effect of the C1 fragment. | Interaction between Shadoo and PrP Affects the PrP-Folding Pathway.
Ciric D, Richard CA, Moudjou M, Chapuis J, Sibille P, Daude N, Westaway D, Adrover M, Béringue V, Martin D, Rezaei H., Free PMC Article | 08/1/2015 |
| Data suggest that glycosylation status of the prion protein and yet-to-be-dentified proteases modulate internal C1 and C2 endoproteolysis of Shadoo (shadow of prion protein) and Doppel (doppelganger prion) in mouse neurons. | Endoproteolytic processing of the mammalian prion glycoprotein family.
Mays CE, Coomaraswamy J, Watts JC, Yang J, Ko KW, Strome B, Mercer RC, Wohlgemuth SL, Schmitt-Ulms G, Westaway D. | 04/12/2014 |
| binding of Sho to anionic liposomes has disruptive effect on integrity of lipid bilayer leading to formation of supramolecular lipid-protein complexes. prion diseases might depend on the oligomerization state of Sho and on these lipoprotein assembles. | Shadoo binds lipid membranes and undergoes aggregation and fibrillization.
Li Q, Chevalier C, Henry C, Richard CA, Moudjou M, Vidic J. | 11/16/2013 |
| Sho is not pi, or, given the accumulating data for many activities for PrP (C) , the pi hypothesis invoking a discrete signaling pathway to maintain neuronal viability is no longer tenable. | Shadoo/PrP (Sprn(0/0) /Prnp(0/0) ) double knockout mice: more than zeroes.
Daude N, Westaway D., Free PMC Article | 08/31/2013 |
| Here we report that Shadoo (Sho)-a member of the prion protein superfamily-is also found in the nucleus of several neural and non-neural cell lines as visualized by using an YFP-Sho construct | The highly conserved, N-terminal (RXXX)8 motif of mouse Shadoo mediates nuclear accumulation.
Tóth E, Kulcsár PI, Fodor E, Ayaydin F, Kalmár L, Borsy AÉ, László L, Welker E. | 06/22/2013 |
| the Sho region N-terminal to the hydrophobic domain includes tandem positively charged "RGG boxes", predicted to bind RNA. Here, we demonstrate that Sho binds DNA and RNA in vitro via this arginine-rich region. | RGG repeats of PrP-like Shadoo protein bind nucleic acids.
Lau A, Mays CE, Genovesi S, Westaway D. | 02/23/2013 |
| using various Prnp transgenic mouse lines and lentiviral vectors expressing shRNAs that target the Shadoo-encoding mRNA, we further demonstrate the specific requirement of at least one of these two PrP-related proteins at early developmental stages | Prion protein and Shadoo are involved in overlapping embryonic pathways and trophoblastic development.
Passet B, Young R, Makhzami S, Vilotte M, Jaffrezic F, Halliez S, Bouet S, Marthey S, Khalifé M, Kanellopoulos-Langevin C, Béringue V, Le Provost F, Laude H, Vilotte JL., Free PMC Article | 01/26/2013 |
| Knockout of the prion protein (PrP)-like Sprn gene does not produce embryonic lethality in combination with PrP(C)-deficiency. | Knockout of the prion protein (PrP)-like Sprn gene does not produce embryonic lethality in combination with PrP(C)-deficiency.
Daude N, Wohlgemuth S, Brown R, Pitstick R, Gapeshina H, Yang J, Carlson GA, Westaway D., Free PMC Article | 09/1/2012 |
| the developmental expression of Shadoo between 10.5 and 14.5 dpc was described. | Expression of the prion-like protein Shadoo in the developing mouse embryo.
Young R, Bouet S, Polyte J, Le Guillou S, Passet B, Vilotte M, Castille J, Beringue V, Le Provost F, Laude H, Vilotte JL. | 01/28/2012 |
| The results of this study concluded that Shadoo has little or no influence on the outcome of transmissible spongiform encephalopathy (TSE) disease in transgenic mice. | Overexpression of Shadoo protein in transgenic mice does not impact the pathogenesis of scrapie.
Wang H, Wan J, Wang W, Wang D, Li S, Liao P, Hao Z, Wu S, Xu J, Li N, Ouyang H, Gao H. | 08/27/2011 |
| Sho may play a role in the physiological function of PrP(C) and prion pathogenesis. | Mapping the interaction site of prion protein and Sho.
Jiayu W, Zhu H, Ming X, Xiong W, Songbo W, Bocui S, Wensen L, Jiping L, Keying M, Zhongyi L, Hongwei G. | 10/4/2010 |
| Shadoo-encoding gene knockdown in PrP-knockout mouse embryos results in a lethal phenotype, occurring between E8 and E11, not observed on the wild-type genetic background. | The prion or the related Shadoo protein is required for early mouse embryogenesis.
Young R, Passet B, Vilotte M, Cribiu EP, Béringue V, Le Provost F, Laude H, Vilotte JL. | 01/21/2010 |
| Sprn does not play a major role in prion disease incubation time in mice. | Shadoo (Sprn) and prion disease incubation time in mice.
Lloyd SE, Grizenkova J, Pota H, Collinge J., Free PMC Article | 01/21/2010 |
| Sho is a candidate for pi, and since it engenders a PrP(C)-like neuroprotective activity, compromised neuroprotective activity resulting from reduced levels exacerbate damage in prion infections. | The CNS glycoprotein Shadoo has PrP(C)-like protective properties and displays reduced levels in prion infections.
Watts JC, Drisaldi B, Ng V, Yang J, Strome B, Horne P, Sy MS, Yoong L, Young R, Mastrangelo P, Bergeron C, Fraser PE, Carlson GA, Mount HT, Schmitt-Ulms G, Westaway D., Free PMC Article | 01/21/2010 |