| RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly. | RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.
Khanduja JS, Joh RI, Perez MM, Paulo JA, Palmieri CM, Zhang J, Gulka AOD, Haas W, Gygi SP, Motamedi M., | 07/3/2024 |
| Spreading and epigenetic inheritance of heterochromatin require a critical density of histone H3 lysine 9 tri-methylation. | Spreading and epigenetic inheritance of heterochromatin require a critical density of histone H3 lysine 9 tri-methylation.
Cutter DiPiazza AR, Taneja N, Dhakshnamoorthy J, Wheeler D, Holla S, Grewal SIS., Free PMC Article | 12/11/2021 |
| Mechanistic Insights into the Allosteric Regulation of the Clr4 Protein Lysine Methyltransferase by Autoinhibition and Automethylation. | Mechanistic Insights into the Allosteric Regulation of the Clr4 Protein Lysine Methyltransferase by Autoinhibition and Automethylation.
Khella MS, Bröhm A, Weirich S, Jeltsch A., Free PMC Article | 05/8/2021 |
| N-Terminus Does Not Govern Protein Turnover of Schizosaccharomyces pombe CENP-A. | N-Terminus Does Not Govern Protein Turnover of Schizosaccharomyces pombe CENP-A.
Tan HL, Zeng YB, Chen ES., Free PMC Article | 02/27/2021 |
| Clr4 chromodomain binds the H3K9me3 tail. Both the chromodomain and the disordered region connecting the chromodomain and the SET domain bind the nucleosome core. Interaction of the disordered region with the nucleosome core is independent of H3K9me and contributes to H3K9me in vitro and in vivo. Those interactions with the nucleosome core contribute to de novo H3K9me deposition and to heterochromatin establishment. | Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity.
Akoury E, Ma G, Demolin S, Brönner C, Zocco M, Cirilo A, Ivic N, Halic M., Free PMC Article | 01/11/2020 |
| revelation of an autoinhibited conformation in the conserved H3K9 methyltransferase Clr4 (also known as Suv39h) of the fission yeast Schizosaccharomyces pombe that has a critical role in preventing aberrant heterochromatin formation | Automethylation-induced conformational switch in Clr4 (Suv39h) maintains epigenetic stability.
Iglesias N, Currie MA, Jih G, Paulo JA, Siuti N, Kalocsay M, Gygi SP, Moazed D., Free PMC Article | 02/23/2019 |
| Survival in quiescence requires the euchromatic deployment of Clr4 by Ago1-associated small RNAs. | Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI, Khanduja JS, Calvo IA, Mistry M, Palmieri CM, Savol AJ, Ho Sui SJ, Sadreyev RI, Aryee MJ, Motamedi M., Free PMC Article | 09/9/2017 |
| These results demonstrate how a direct "read-write" mechanism involving Clr4 propagates histone modifications and allows histones to act as carriers of epigenetic information. | Epigenetics. Epigenetic inheritance uncoupled from sequence-specific recruitment.
Ragunathan K, Jih G, Moazed D., Free PMC Article | 05/2/2015 |
| Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly. | Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.
Alper BJ, Job G, Yadav RK, Shanker S, Lowe BR, Partridge JF., Free PMC Article | 11/30/2013 |
| Division of Labor between the Chromodomains of HP1/Swi6 and Suv39/Clr4 Is Required for Productive Heterochromatin Assembly. | Division of labor between the chromodomains of HP1 and Suv39 methylase enables coordination of heterochromatin spread.
Al-Sady B, Madhani HD, Narlikar GJ., Free PMC Article | 10/19/2013 |
| Data show that although Ers1 failed to bind Clr4, a direct interaction with Swi6 was detected, and centromeric localization of Swi6 was enhanced by Clr4 overexpression. | Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.
Hayashi A, Ishida M, Kawaguchi R, Urano T, Murakami Y, Nakayama J., Free PMC Article | 07/14/2012 |
| self-association of Swi6/HP1 helps in binding to and recruitment of Clr4 and thereby in establishment and maintenance of heterochromatin by a concerted rather than a sequential mechanism. | Role of Swi6/HP1 self-association-mediated recruitment of Clr4/Suv39 in establishment and maintenance of heterochromatin in fission yeast.
Haldar S, Saini A, Nanda JS, Saini S, Singh J., Free PMC Article | 05/21/2011 |
| findings link Clr4 to RNA quality control machinery and suggest a pathway for processing potentially deleterious RNAs through the coordinated actions of RNAi and other RNA processing activities | Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.
Zhang K, Fischer T, Porter RL, Dhakshnamoorthy J, Zofall M, Zhou M, Veenstra T, Grewal SI., Free PMC Article | 04/9/2011 |
| Data show that overexpression of clr4(+) in clr4Deltaago1Delta cells results in some de novo H3K9me2 accumulation at centromeres. | Continuous requirement for the Clr4 complex but not RNAi for centromeric heterochromatin assembly in fission yeast harboring a disrupted RITS complex.
Shanker S, Job G, George OL, Creamer KM, Shaban A, Partridge JF., Free PMC Article | 03/12/2011 |
| Data show that the stretched morphology of lagging kinetochores in pcs1Delta and clr4Delta mutant cells is due to merotelic attachment. | Laser microsurgery provides evidence for merotelic kinetochore attachments in fission yeast cells lacking Pcs1 or Clr4.
Rumpf C, Cipak L, Schleiffer A, Pidoux A, Mechtler K, Tolić-Nørrelykke IM, Gregan J., Free PMC Article | 03/5/2011 |
| The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation. | The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.
Gerace EL, Halic M, Moazed D., Free PMC Article | 09/13/2010 |
| The components of the Clr4 histone K3-K9 methyltransferase-Rik1-Cullin4 complex act concertedly at an early step in heterochromatin formation. | A cullin E3 ubiquitin ligase complex associates with Rik1 and the Clr4 histone H3-K9 methyltransferase and is required for RNAi-mediated heterochromatin formation.
Hong EJ, Villén J, Gerace EL, Gygi SP, Moazed D. | 01/21/2010 |
| APC and heterochromatin proteins Swi6 and Clr4 play a mutually cooperative role in heterochromatin assembly, thereby ensuring chromosomal integrity, inheritance, and segregation during mitosis and meiosis. | Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast.
Dubey RN, Nakwal N, Bisht KK, Saini A, Haldar S, Singh J., Free PMC Article | 01/21/2010 |
| analyses delineate sequential steps for the assembly of heterochromatic domains and suggest that the ability of Clr4 to both 'write' and 'read' H3K9me facilitates heterochromatin maintenance through successive cell divisions | Roles of the Clr4 methyltransferase complex in nucleation, spreading and maintenance of heterochromatin.
Zhang K, Mosch K, Fischle W, Grewal SI. | 01/21/2010 |
| In a strain lacking the histone methyltransferase Clr4, crucial for the formation of heterochromatin, the mating-type region had a random localization in the nucleus. | The Clr4 methyltransferase determines the subnuclear localization of the mating-type region in fission yeast.
Alfredsson-Timmins J, Henningson F, Bjerling P. | 01/21/2010 |
| analysis of transcriptional targets of the histone H3 Lys9-specific methyltransferase Clr4 by mrna differential display | Silencing in yeast: identification of clr4 targets.
Ivanov SV, Ivanova AV. | 01/21/2010 |
| Clr4 abolished the inhibitory effect of histone h3 on the RanGAP activity required for the heterochromatin assembly. | Nuclear RanGAP is required for the heterochromatin assembly and is reciprocally regulated by histone H3 and Clr4 histone methyltransferase in Schizosaccharomyces pombe.
Nishijima H, Nakayama J, Yoshioka T, Kusano A, Nishitani H, Shibahara K, Nishimoto T., Free PMC Article | 01/21/2010 |