| Cdk1 phosphorylation of formin Cdc12 antagonizes its interaction with Cdc15 and thereby opposes Cdc12's contractile ring localization. These results are consistent with a general role for Cdk1 in inhibiting cytokinesis until chromosome segregation is complete. | Cdk1-dependent phosphoinhibition of a formin-F-BAR interaction opposes cytokinetic contractile ring formation.
Willet AH, Bohnert KA, Gould KL., Free PMC Article | 02/9/2019 |
| Cdc12 mechanoregulation facilitates actomyosin ring assembly. | Mechanoregulated inhibition of formin facilitates contractile actomyosin ring assembly.
Zimmermann D, Homa KE, Hocky GM, Pollard LW, De La Cruz EM, Voth GA, Trybus KM, Kovar DR., Free PMC Article | 02/10/2018 |
| Data indicate that the essential role of profilin is to specifically facilitate formin Cdc12-mediated actin assembly for cytokinesis. | Fission yeast profilin is tailored to facilitate actin assembly by the cytokinesis formin Cdc12.
Bestul AJ, Christensen JR, Grzegorzewska AP, Burke TA, Sees JA, Carroll RT, Sirotkin V, Keenan RJ, Kovar DR., Free PMC Article | 10/3/2015 |
| this paper defines a short motif within the N terminus of Cdc12 that binds directly to the F-BAR domain of the scaffolding protein Cdc15. | The F-BAR Cdc15 promotes contractile ring formation through the direct recruitment of the formin Cdc12.
Willet AH, McDonald NA, Bohnert KA, Baird MA, Allen JR, Davidson MW, Gould KL., Free PMC Article | 05/2/2015 |
| Cdc12 and for3 cooperate in cytokinesis and de novo actin assembly at the division site is predominant for contractile ring formation. | The formins Cdc12 and For3 cooperate during contractile ring assembly in cytokinesis.
Coffman VC, Sees JA, Kovar DR, Wu JQ., Free PMC Article | 12/7/2013 |
| Cdc12 is an efficient nucleator (one filament per approximately 3 Cdc12 dimers) that processively elongates profilin-actin at a moderate rate of 10 subunits s-1 muM-1, but lacks filament-bundling activity. | The functionally distinct fission yeast formins have specific actin-assembly properties.
Scott BJ, Neidt EM, Kovar DR., Free PMC Article | 02/18/2012 |
| cdc12p contributes at least two separable activities: actin assembly and one or more additional functions in cytokinesis initiation | Regulation of cytokinesis by the formin cdc12p.
Yonetani A, Chang F., Free PMC Article | 07/5/2010 |
| Live microscopy of cells expressing Cdc12p fluorescent fusion proteins shows for the first time that Cdc12p localizes to a broad band of 30-50 dynamic nodes, where actin filaments are nucleated in random directions. | Roles of formin nodes and myosin motor activity in Mid1p-dependent contractile-ring assembly during fission yeast cytokinesis.
Coffman VC, Nile AH, Lee IJ, Liu H, Wu JQ., Free PMC Article | 03/15/2010 |
| Cdc12p can recruit SpTm to actin filaments and SpTm has diverse effects on Cdc12p-mediated actin assembly. | Role of tropomyosin in formin-mediated contractile ring assembly in fission yeast.
Skau CT, Neidt EM, Kovar DR., Free PMC Article | 01/21/2010 |
| most likely hypotheses to explain the incompatibility of human profilin-I with Cdc12p are differences in interactions with the proline-rich sequences in the FH1 domain of Cdc12p and wider "wings" that interact with actin | Incompatibility with formin Cdc12p prevents human profilin from substituting for fission yeast profilin: insights from crystal structures of fission yeast profilin.
Ezezika OC, Younger NS, Lu J, Kaiser DA, Corbin ZA, Nolen BJ, Kovar DR, Pollard TD., Free PMC Article | 01/21/2010 |
| A mutational analysis of cdc12p was used to identify regions of cdc12p responsible for ring assembly and localization. | Regulation and targeting of the fission yeast formin cdc12p in cytokinesis.
Yonetani A, Lustig RJ, Moseley JB, Takeda T, Goode BL, Chang F., Free PMC Article | 01/21/2010 |
| Both Cdc12p and CYK-1 bind to pre-assembled actin filaments with low nanomolar affinity, but CYK-1 dissociates 2 orders of magnitude more quickly. | The cytokinesis formins from the nematode worm and fission yeast differentially mediate actin filament assembly.
Neidt EM, Skau CT, Kovar DR., Free PMC Article | 01/21/2010 |