| History of DNA polymerase beta X-ray crystallography. | History of DNA polymerase β X-ray crystallography.
Whitaker AM, Freudenthal BD., Free PMC Article | 03/28/2021 |
| The I260Q variant of DNA polymerase beta is an efficient mutator polymerase with fairly indiscriminate misincorporation activities opposite all template bases. | Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator DNA polymerase β.
Gridley CL, Rangarajan S, Firbank S, Dalal S, Sweasy JB, Jaeger J., Free PMC Article | 08/31/2013 |
| The closed state of E295K has a more distorted active site than the active site in the wild-type Polb. | Unfavorable electrostatic and steric interactions in DNA polymerase β E295K mutant interfere with the enzyme's pathway.
Li Y, Gridley CL, Jaeger J, Sweasy JB, Schlick T., Free PMC Article | 10/20/2012 |
| decline in pol beta activity started between 30 and 45 days postnatal in all the tissues. Post mitotic tissues showed pronounced decline than the proliferating tissues | Age-related decline in DNA polymerase β activity in rat brain and tissues.
Vyjayanti VN, Swain U, Rao KS. | 08/25/2012 |
| at low pH the chemical step is rate limiting for catalysis, but at high pH, a postchemistry conformational step is rate limiting due to a pH-dependent increase in the rate of nucleotidyl transfer | Kinetic mechanism of active site assembly and chemical catalysis of DNA polymerase β.
Balbo PB, Wang EC, Tsai MD. | 01/7/2012 |
| These results point to the hinge region of DNA polymerase beta as being critical in the maintenance of the proper geometry of the dNTP binding pocket. | Hinge residue I174 is critical for proper dNTP selection by DNA polymerase beta.
Yamtich J, Starcevic D, Lauper J, Smith E, Shi I, Rangarajan S, Jaeger J, Sweasy JB., Free PMC Article | 04/12/2010 |
| NMR assignments of the full-length protein (335 residues, 39 kDa) in the presence of a double gap--double hairpin DNA (22 nucleotides, 7 kDa) | NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA.
Mueller GA, DeRose EF, Kirby TW, London RE., Free PMC Article | 01/21/2010 |
| for Pol beta, fidelity is dictated by the differences in free energy required to reach the highest energy transition state of the chemical step. | Contribution of the reverse rate of the conformational step to polymerase beta fidelity.
Bakhtina M, Roettger MP, Tsai MD. | 01/21/2010 |
| Loop II of pol beta functions to maintain accurate DNA synthesis by a direct or indirect influence on the nucleotide binding pocket. | Loop II of DNA polymerase beta is important for discrimination during substrate binding.
Lin GC, Jaeger J, Eckert KA, Sweasy JB., Free PMC Article | 01/21/2010 |
| Changes in the activity and expression of DNA polymerase beta are mediated via cAMP and the A-kinase system, and that phosphorylation of this enzyme is also involved in this expression. | Hormonal regulation of DNA polymerase beta activity and expression in rat adrenal glands and testes.
Kotake M, Nakai A, Nagasaka A, Itoh M, Hidaka H, Yoshida S. | 01/21/2010 |
| The upregulation of base-excision repair activities after ischemic preconditioning was likely because of increased expression of DNA Polymerase beta. | Ischemic preconditioning in the rat brain enhances the repair of endogenous oxidative DNA damage by activating the base-excision repair pathway.
Li W, Luo Y, Zhang F, Signore AP, Gobbel GT, Simon RP, Chen J. | 01/21/2010 |
| data support a role for the flexible loop in pol beta error discrimination | Effect of DNA polymerase beta loop variants on discrimination of O6-methyldeoxyguanosine modification present in the nucleotide versus template substrate.
Hamid S, Eckert KA. | 01/21/2010 |
| DNA polymerase beta discriminates the correct from incorrect substrate during the binding step. | The hydrophobic hinge region of rat DNA polymerase beta is critical for substrate binding pocket geometry.
Starcevic D, Dalal S, Jaeger J, Sweasy JB. | 01/21/2010 |
| Lys, Arg, Asp, Asn, and Glu substitutions for Ile at hinge residue 260 of pol beta yield enzymes with impaired activity or accuracy. | Hinge residue Ile260 of DNA polymerase beta is important for enzyme activity and fidelity.
Starcevic D, Dalal S, Sweasy J. | 01/21/2010 |
| describe a site-directed mutational analysis in which the key amino acids (L11, K35, H51, K60, L77, and T79), which are direct interaction sites in the domain, were substituted with K, A, A, A, K, and A, respectively | Site-directed mutational analysis of structural interactions of low molecule compounds binding to the N-terminal 8 kDa domain of DNA polymerase beta.
Murakami S, Kamisuki S, Takata K, Kasai N, Kimura S, Mizushina Y, Ohta K, Sugawara F, Sakaguchi K. | 01/21/2010 |
| pol beta may have a role in UV lesion bypass and deregulated pol beta may enhance UV-induced genetic instability | A role for DNA polymerase beta in mutagenic UV lesion bypass.
Servant L, Cazaux C, Bieth A, Iwai S, Hanaoka F, Hoffmann JS. | 01/21/2010 |
| pol beta binds to dsDNA oligomers with the site-size of the enzyme-dsDNA complex n = 5 +/- 1 base pairs | Rat polymerase beta binds double-stranded DNA using exclusively the 8-kDa domain. Stoichiometries, intrinsic affinities, and cooperativities.
Jezewska MJ, Galletto R, Bujalowski W. | 01/21/2010 |
| single stranded DNA interactions and kinetics | Kinetic mechanisms of rat polymerase beta-ssDNA interactions. Quantitative fluorescence stopped-flow analysis of the formation of the (Pol beta)(16) and (Pol beta)(5) ssDNA binding mode.
Jezewska MJ, Rajendran S, Galletto R, Bujalowski W. | 01/21/2010 |
| the 8-kDa domain had two inhibitor binding areas; Three amino acid residues (Lys60, Leu77, and Thr79) of the 8-kDa domain bound to LCA and compound 2 and four amino acid residues (Leu11, Lys35, His51, and Thr79) of the 8-kDa domain bound to compound 9. | Structural relationship of lithocholic acid derivatives binding to the N-terminal 8-kDa domain of DNA polymerase beta.
Mizushina Y, Kasai N, Miura K, Hanashima S, Takemura M, Yoshida H, Sugawara F, Sakaguchi K. | 01/21/2010 |
| data show that changes in liver expression of DNA polymerase beta, Ref-1, proliferating cell nuclear antigen, and Bax are associated with peroxisome proliferator-induced carcinogenesis in rats but not in hamsters. | Hepatic expression of polymerase beta, Ref-1, PCNA, and Bax in WY 14,643-exposed rats and hamsters.
Holmes EW, Bingham CM, Cunningham ML. | 01/21/2010 |
| the subdomain-closing conformational change occurs before binding of the catalytic Mg(II) while the rate-limiting step occurs after binding of the catalytic Mg(II) | Use of viscogens, dNTPalphaS, and rhodium(III) as probes in stopped-flow experiments to obtain new evidence for the mechanism of catalysis by DNA polymerase beta.
Bakhtina M, Lee S, Wang Y, Dunlap C, Lamarche B, Tsai MD. | 01/21/2010 |
| NMR data indicate that the nucleotide-DNA interaction appears to be essential for conformational activation | Dynamic characterization of a DNA repair enzyme: NMR studies of [methyl-13C]methionine-labeled DNA polymerase beta.
Bose-Basu B, DeRose EF, Kirby TW, Mueller GA, Beard WA, Wilson SH, London RE. | 01/21/2010 |