| Regulation of the Drosophila transcriptome by Pumilio and the CCR4-NOT deadenylase complex. | Regulation of the Drosophila transcriptome by Pumilio and the CCR4-NOT deadenylase complex.
Haugen RJ, Barnier C, Elrod ND, Luo H, Jensen MK, Ji P, Smibert CA, Lipshitz HD, Wagner EJ, Freddolino PL, Goldstrohm AC., | 07/2/2024 |
| A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs. | A conserved domain of Drosophila RNA-binding protein Pumilio interacts with multiple CCR4-NOT deadenylase complex subunits to repress target mRNAs.
Haugen RJ, Arvola RM, Connacher RP, Roden RT, Goldstrohm AC., Free PMC Article | 10/22/2022 |
| Structure and dynamics of the quaternary hunchback mRNA translation repression complex. | Structure and dynamics of the quaternary hunchback mRNA translation repression complex.
Macošek J, Simon B, Linse JB, Jagtap PKA, Winter SL, Foot J, Lapouge K, Perez K, Rettel M, Ivanović MT, Masiewicz P, Murciano B, Savitski MM, Loedige I, Hub JS, Gabel F, Hennig J., Free PMC Article | 11/6/2021 |
| Myocyte enhancer factor-2 and p300 interact to regulate the expression of homeostatic regulator Pumilio in Drosophila. | Myocyte enhancer factor-2 and p300 interact to regulate the expression of homeostatic regulator Pumilio in Drosophila.
Lin WH, Baines RA., Free PMC Article | 08/15/2020 |
| Findings identify Bag-of-marbles (Bam) as a new pumilio (Pum) cofactor in repression of Mothers against dpp (mad) 3' UTR in Schneider's 2 cells (S2 cells). | The target specificity of the RNA binding protein Pumilio is determined by distinct co-factors.
Malik S, Jang W, Park SY, Kim JY, Kwon KS, Kim C., Free PMC Article | 08/12/2020 |
| This study explores the crystallization and structure determination of a Pum-Nos-RNA complex. | Preparation of cooperative RNA recognition complexes for crystallographic structural studies.
Qiu C, Goldstrohm AC, Tanaka Hall TM., Free PMC Article | 05/23/2020 |
| the catalytic activity of Pop2 deadenylase is important for Pumilio RD activity. Further, we show that the Pumilio RDs directly bind to the CNOT complex. We also report that the decapping enzyme, Dcp2, participates in repression by the N-terminus of Pumilio. These results support a model wherein Pumilio utilizes CNOT deadenylase and decapping complexes to accelerate destruction of target mRNAs. | Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs.
Arvola RM, Chang CT, Buytendorp JP, Levdansky Y, Valkov E, Freddolino PL, Goldstrohm AC., Free PMC Article | 03/21/2020 |
| Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor proteins has been described. | Combinatorial control of messenger RNAs by Pumilio, Nanos and Brain Tumor Proteins.
Arvola RM, Weidmann CA, Tanaka Hall TM, Goldstrohm AC., Free PMC Article | 07/21/2018 |
| The specificity of a complex between Drosophila RNA-binding protein Pumilio and its target RNA of Nanos has been described. | Integrated analysis of RNA-binding protein complexes using in vitro selection and high-throughput sequencing and sequence specificity landscapes (SEQRS).
Lou TF, Weidmann CA, Killingsworth J, Tanaka Hall TM, Goldstrohm AC, Campbell ZT., Free PMC Article | 02/24/2018 |
| Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity. | Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio.
Weidmann CA, Qiu C, Arvola RM, Lou TF, Killingsworth J, Campbell ZT, Tanaka Hall TM, Goldstrohm AC., Free PMC Article | 10/21/2017 |
| reductions in the expression of the Pumilio translational repressor complex enhanced phenotypes due to dLsd1 depletion in Drosophila. the PUM complex is a target of LSD1 regulation. | The LSD1 Family of Histone Demethylases and the Pumilio Posttranscriptional Repressor Function in a Complex Regulatory Feedback Loop.
Miles WO, Lepesant JM, Bourdeaux J, Texier M, Kerenyi MA, Nakakido M, Hamamoto R, Orkin SH, Dyson NJ, Di Stefano L., Free PMC Article | 05/7/2016 |
| BRAT is a prominent post-transcriptional regulator in the early embryo through mechanisms that are largely independent of PUM. | Brain tumor is a sequence-specific RNA-binding protein that directs maternal mRNA clearance during the Drosophila maternal-to-zygotic transition.
Laver JD, Li X, Ray D, Cook KB, Hahn NA, Nabeel-Shah S, Kekis M, Luo H, Marsolais AJ, Fung KY, Hughes TR, Westwood JT, Sidhu SS, Morris Q, Lipshitz HD, Smibert CA., Free PMC Article | 02/27/2016 |
| Pumilio has a novel mRNA decay function during neural development. Decreased expression stabilized predicted neural mRNA targets. A Pumilio recognition element is needed to trigger reporter-transcript decay in the nervous system. | Dynamic regulation of mRNA decay during neural development.
Burow DA, Umeh-Garcia MC, True MB, Bakhaj CD, Ardell DH, Cleary MD., Free PMC Article | 01/23/2016 |
| Nos and Pum are required to maintain the dendritic complexity of da neurons during larval growth by promoting the outgrowth of new dendritic branches and the stabilization of existing dendritic branches | Combinatorial use of translational co-factors for cell type-specific regulation during neuronal morphogenesis in Drosophila.
Olesnicky EC, Bhogal B, Gavis ER., Free PMC Article | 06/29/2013 |
| Evidence suggests that Pum functions as a negative regulator of EGFR signaling by directly targeting components of the pathway in Drosophila. | Negative regulation of EGFR/MAPK pathway by Pumilio in Drosophila melanogaster.
Kim SY, Kim JY, Malik S, Son W, Kwon KS, Kim C., Free PMC Article | 09/1/2012 |
| Pumilio complexes repress dE2F1 levels | Pumilio facilitates miRNA regulation of the E2F3 oncogene.
Miles WO, Tschöp K, Herr A, Ji JY, Dyson NJ., Free PMC Article | 04/7/2012 |
| Cofactors Brain Tumor and eIF4E Homologous Protein are not obligatory for Pumilio and Nanos activity. | Drosophila Pumilio protein contains multiple autonomous repression domains that regulate mRNAs independently of Nanos and brain tumor.
Weidmann CA, Goldstrohm AC., Free PMC Article | 02/25/2012 |
| Data show that the extended 3' UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3' lengthening phenomenon. | Neural-specific elongation of 3' UTRs during Drosophila development.
Hilgers V, Perry MW, Hendrix D, Stark A, Levine M, Haley B., Free PMC Article | 12/17/2011 |
| The studies suggest that, in cystoblasts, Bam and Bgcn may directly inhibit Pum/Nos activity to promote differentiation of germ line stem cells. | Direct inhibition of Pumilo activity by Bam and Bgcn in Drosophila germ line stem cell differentiation.
Kim JY, Lee YC, Kim C., Free PMC Article | 05/31/2010 |
| Regulatory interplay between Nanos and Pum could cause a small change in Pum activity to be amplified into a large shift in the balance between GluRIIA and GluRIIB synapses. | The translational repressors Nanos and Pumilio have divergent effects on presynaptic terminal growth and postsynaptic glutamate receptor subunit composition.
Menon KP, Andrews S, Murthy M, Gavis ER, Zinn K., Free PMC Article | 01/21/2010 |
| a translaitonal repressor that regulates neuronal excitability | The Drosophila melanogaster translational repressor pumilio regulates neuronal excitability.
Schweers BA, Walters KJ, Stern M., Free PMC Article | 01/21/2010 |
| affects anterior determination in drosophila embryo; temporally regulates bicoid (bcd) mRNA translation via evolutionarily conserved Nanos response elements (NRE) in its 3'UTR | An anterior function for the Drosophila posterior determinant Pumilio.
Gamberi C, Peterson DS, He L, Gottlieb E. | 01/21/2010 |
| role in long-term memory | The staufen/pumilio pathway is involved in Drosophila long-term memory.
Dubnau J, Chiang AS, Grady L, Barditch J, Gossweiler S, McNeil J, Smith P, Buldoc F, Scott R, Certa U, Broger C, Tully T. | 01/21/2010 |
| Pumilio represses translation of differentiation-promoting mRNAs in germline stem cells. | Gene circuitry controlling a stem cell niche.
Chen D, McKearin D. | 01/21/2010 |
| A characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO was identified. | Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.
Gerber AP, Luschnig S, Krasnow MA, Brown PO, Herschlag D., Free PMC Article | 01/21/2010 |