| Conformational Selection Mechanism Provides Structural Insights into the Optimization of APC-Asef Inhibitors. | Conformational Selection Mechanism Provides Structural Insights into the Optimization of APC-Asef Inhibitors.
He X, Huang N, Qiu Y, Zhang J, Liu Y, Yin XL, Lu S., Free PMC Article | 04/13/2021 |
| The armadillo repeats in full-length APC interact with the APC residues 1362 to 1540 (APC-2,3 repeats), and this interaction competes off and inhibits Asef. Deletion of APC-2,3 repeats permits Asef interactions leading to downstream signaling events, including the induction of Golgi fragmentation through the activation of the Asef-ROCK-MLC2. | Truncated Adenomatous Polyposis Coli Mutation Induces Asef-Activated Golgi Fragmentation.
Kim SB, Zhang L, Yoon J, Lee J, Min J, Li W, Grishin NV, Moon YA, Wright WE, Shay JW., Free PMC Article | 06/29/2019 |
| Evaluation of an immunohistochemical staining of 102 resected pancreatic cancer samples demonstrated that high ARHGEF4 expression was correlated with an independent predictor of worse overall survival. ARHGEF4 induces the formation of cell protrusions by increasing phosphorylated ERK1/2 and GSK-3alpha/beta, resulting in an increase in motility and invasiveness in pancreatic ductal adenocarcinoma cells. | ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer.
Taniuchi K, Furihata M, Naganuma S, Saibara T. | 02/2/2019 |
| Our results indicate a possible contribution of CNVs in LEPR, NEGR1, ARHGEF4, and CPXCR1 and the intergenic regions 12q15c, 15q21.1a, and 22q11.21d to the development of obesity, particularly abdominal obesity in Mexican children. | Copy Number Variations in Candidate Genes and Intergenic Regions Affect Body Mass Index and Abdominal Obesity in Mexican Children.
Antúnez-Ortiz DL, Flores-Alfaro E, Burguete-García AI, Bonnefond A, Peralta-Romero J, Froguel P, Espinoza-Rojo M, Cruz M., Free PMC Article | 03/3/2018 |
| Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor | Quantitative genome-wide methylation analysis of high-grade non-muscle invasive bladder cancer.
Kitchen MO, Bryan RT, Emes RD, Glossop JR, Luscombe C, Cheng KK, Zeegers MP, James ND, Devall AJ, Mein CA, Gommersall L, Fryer AA, Farrell WE., Free PMC Article | 05/27/2017 |
| These data demonstrate, that in addition to microtubule-independent Tiam1 activation, HGF engages additional microtubule- and APC-dependent pathway of Asef activation. | Hepatocyte growth factor triggers distinct mechanisms of Asef and Tiam1 activation to induce endothelial barrier enhancement.
Higginbotham K, Tian Y, Gawlak G, Moldobaeva N, Shah A, Birukova AA., Free PMC Article | 05/16/2015 |
| these results demonstrate potent anti-inflammatory effects by HGF and delineate a key role of Asef in the mediation of the HGF barrier protective and anti-inflammatory effects. | Asef mediates HGF protective effects against LPS-induced lung injury and endothelial barrier dysfunction.
Meng F, Meliton A, Moldobaeva N, Mutlu G, Kawasaki Y, Akiyama T, Birukova AA., Free PMC Article | 05/2/2015 |
| Expression of Asef was associated with lower estimated glomerular filtration rate in kidney disease. | Association of Asef and Cdc42 expression to tubular injury in diseased human kidney.
Cheng HT, Juang IP, Chen LC, Lin LY, Chao CH. | 07/19/2014 |
| Expression of ARHGEF4 (Rho guanine nucleotide exchange factor 4) is restricted to the brain and may regulate the actin cytoskeletal network, cell morphology and migration, and neuronal function | Small rare recurrent deletions and reciprocal duplications in 2q21.1, including brain-specific ARHGEF4 and GPR148.
Dharmadhikari AV, Kang SH, Szafranski P, Person RE, Sampath S, Prakash SK, Bader PI, Phillips JA 3rd, Hannig V, Williams M, Vinson SS, Wilfong AA, Reimschisel TE, Craigen WJ, Patel A, Bi W, Lupski JR, Belmont J, Cheung SW, Stankiewicz P., Free PMC Article | 06/29/2013 |
| structures thus elucidate the molecular mechanism of Asef recognition by APC | Structural basis for the recognition of Asef by adenomatous polyposis coli.
Zhang Z, Chen L, Gao L, Lin K, Zhu L, Lu Y, Shi X, Gao Y, Zhou J, Xu P, Zhang J, Wu G., Free PMC Article | 05/26/2012 |
| Forced expression of Asef in vitro induced and maintained proliferation of haemaopoietic progenitor cells and co-operated with TEL-AML1 greatly to enhance proliferation and haemopoietic colony size | The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation.
Lyons R, Williams O, Morrow M, Sebire N, Hubank M, Anderson J. | 02/22/2010 |
| Asef and APC function downstream of HGF and PI3-kinase, and play critical roles in growth factor-mediated regulation of cell morphology and migration | Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase.
Kawasaki Y, Tsuji S, Sagara M, Echizen K, Shibata Y, Akiyama T., Free PMC Article | 01/21/2010 |
| Positive role of Tyr94 phosphorylation in EGF-induced Asef activation following the activation of Rac1. | Phosphorylation and activation of the Rac1 and Cdc42 GEF Asef in A431 cells stimulated by EGF.
Itoh RE, Kiyokawa E, Aoki K, Nishioka T, Akiyama T, Matsuda M. | 01/21/2010 |