| Our findings suggest that CHT protein levels preferentially impact the capacity for ACh release afforded by mobilization of reserve pool vesicles | Differential impact of genetically modulated choline transporter expression on the release of endogenous versus newly synthesized acetylcholine.
Iwamoto H, Calcutt MW, Blakely RD., Free PMC Article | 01/13/2018 |
| These data provide evidence for an epithelium-independent non-vesicular, non-quantal ACh release in the mouse trachea involving low-affinity choline transporters. | Cholinergic activation of the murine trachealis muscle via non-vesicular acetylcholine release involving low-affinity choline transporters.
Nassenstein C, Wiegand S, Lips KS, Li G, Klein J, Kummer W. | 09/17/2016 |
| bacterial artificial chromosome CHT mice thus represent a novel tool to examine both the positive and negative impact of constitutively elevated cholinergic signaling capacity | Transgenic overexpression of the presynaptic choline transporter elevates acetylcholine levels and augments motor endurance.
Holmstrand EC, Lund D, Cherian AK, Wright J, Martin RF, Ennis EA, Stanwood GD, Sarter M, Blakely RD., Free PMC Article | 02/7/2015 |
| These findings reveal compensatory mechanisms that, in the context of moderate cognitive challenges, can overcome the performance deficits expected from the significantly reduced ACh capacity of CHT+/- cholinergic terminals. | Monitoring cholinergic activity during attentional performance in mice heterozygous for the choline transporter: a model of cholinergic capacity limits.
Paolone G, Mallory CS, Cherian AK, Miller TR, Blakely RD, Sarter M., Free PMC Article | 08/23/2014 |
| CHT association with cholesterol-rich rafts is critical for its function | Regulation of the high-affinity choline transporter activity and trafficking by its association with cholesterol-rich lipid rafts.
Cuddy LK, Winick-Ng W, Rylett RJ. | 05/3/2014 |
| This study showed that a constitutive loss of CHT expression in hemizygous mice leads to a significant reduction in basal extracellular dopamine levels in the nucleus accumbens. | Choline transporter hemizygosity results in diminished basal extracellular dopamine levels in nucleus accumbens and blunts dopamine elevations following cocaine or nicotine.
Dong Y, Dani JA, Blakely RD., Free PMC Article | 12/21/2013 |
| This study demonistrated that CHT support for neuromuscular signaling involves contributions to ACh synthesis as well as cholinergic synaptic vesicle availability. | Motor neuron-specific overexpression of the presynaptic choline transporter: impact on motor endurance and evoked muscle activity.
Lund D, Ruggiero AM, Ferguson SM, Wright J, English BA, Reisz PA, Whitaker SM, Peltier AC, Blakely RD., Free PMC Article | 03/12/2011 |
| CHT1 knockout mice have tachycardia and hypertension at rest. | Tachycardia, reduced vagal capacity, and age-dependent ventricular dysfunction arising from diminished expression of the presynaptic choline transporter.
English BA, Appalsamy M, Diedrich A, Ruggiero AM, Lund D, Wright J, Keller NR, Louderback KM, Robertson D, Blakely RD., Free PMC Article | 09/27/2010 |
| Synapses persist for at least 2-3 months without postsynaptic activity; however, the presynaptic terminals lack high-affinity choline transporters (CHTs), and as a result, they are quickly depleted of transmitter. | An activity-dependent retrograde signal induces the expression of the high-affinity choline transporter in cholinergic neurons.
Krishnaswamy A, Cooper E. | 01/21/2010 |
| adaptor proteins have important functions for CHT trafficking in neuronal cells | Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice.
Misawa H, Fujigaya H, Nishimura T, Moriwaki Y, Okuda T, Kawashima K, Nakata K, Ruggiero AM, Blakely RD, Nakatsu F, Ohno H. | 01/21/2010 |
| Choline transporter hemizygosity results in adequate baseline acetylcholine stores, sufficient to sustain many phenotypes, but normal sensitivities to physical and/or pharmacological challenge require full cholinergic signaling capacity. | Deficits in acetylcholine homeostasis, receptors and behaviors in choline transporter heterozygous mice.
Bazalakova MH, Wright J, Schneble EJ, McDonald MP, Heilman CJ, Levey AI, Blakely RD. | 01/21/2010 |
| CHT1 was expressed early in CNS development, predominantly in the regions containing cholinergic neurons. NGF AND BMP4 increased CHT mRNA accumulation in primary septal cultures. | Expression of high affinity choline transporter during mouse development in vivo and its upregulation by NGF and BMP-4 in vitro.
Berse B, Szczecinska W, Lopez-Coviella I, Madziar B, Zemelko V, Kaminski R, Kozar K, Lips KS, Pfeil U, Blusztajn JK. | 01/21/2010 |
| CHT is an essential and regulated presynaptic component of cholinergic signaling | Lethal impairment of cholinergic neurotransmission in hemicholinium-3-sensitive choline transporter knockout mice.
Ferguson SM, Bazalakova M, Savchenko V, Tapia JC, Wright J, Blakely RD., Free PMC Article | 01/21/2010 |
| Real-time RT-PCR revealed significant decreases in CHT1 mRNA in the lung of sensitized and allergen challenged animals. | Down-regulation of the non-neuronal acetylcholine synthesis and release machinery in acute allergic airway inflammation of rat and mouse.
Lips KS, Lührmann A, Tschernig T, Stoeger T, Alessandrini F, Grau V, Haberberger RV, Koepsell H, Pabst R, Kummer W. | 01/21/2010 |
| CHT1 binds to Par-4 and inhibits choline uptake when its incorporation is reduced on the cell surface | Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane.
Xie J, Guo Q. | 01/21/2010 |
| Interestingly, although CHT is expressed predominantly in cholinergic cells, its regulation appears to be distinct from that of the cholinergic locus. | Differential regulation of the high affinity choline transporter and the cholinergic locus by cAMP signaling pathways.
Brock M, Nickel AC, Madziar B, Blusztajn JK, Berse B., Free PMC Article | 01/21/2010 |