| these data define a novel molecular mechanism underlying the assembly of CENP-T onto the centromere by a temporally regulated HJURP-CENP-T interaction. | Holliday junction recognition protein interacts with and specifies the centromeric assembly of CENP-T.
Ding M, Jiang J, Yang F, Zheng F, Fang J, Wang Q, Wang J, Yao W, Liu X, Gao X, Mullen M, He P, Rono C, Ding X, Hong J, Fu C, Liu X, Yao X., Free PMC Article | 04/20/2019 |
| Levels of centromere aberrations increase upon depletion of CENP-A, CENP-C, and CENP-T/W, during replicative senescence, and in cancer cells. | Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, and CENP-T.
Giunta S, Funabiki H., Free PMC Article | 04/14/2018 |
| report a novel disease gene encoding the constitutive inner kinetochore member CENPT, which is involved in kinetochore targeting and assembly, resulting in severe growth failure in two siblings of a consanguineous family | A defect in the inner kinetochore protein CENPT causes a new syndrome of severe growth failure.
Hung CY, Volkmar B, Baker JD, Bauer JW, Gussoni E, Hainzl S, Klausegger A, Lorenzo J, Mihalek I, Rittinger O, Tekin M, Dallman JE, Bodamer OA., Free PMC Article | 01/6/2018 |
| Upon cross-linking, the entire CENPA/CENPB/CENPC/CENPT complex is nuclease-protected over an alpha-satellite dimer that comprises the fundamental unit of centromeric chromatin. We conclude that CENPA/CENPC and CENPT pathways for kinetochore assembly are physically integrated over young alpha-satellite dimers. | CENPT bridges adjacent CENPA nucleosomes on young human α-satellite dimers.
Thakur J, Henikoff S., Free PMC Article | 01/6/2018 |
| Whereas CENP-C recruits a single MIS12:NDC80 complex, the authors show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. | Molecular basis of outer kinetochore assembly on CENP-T.
Huis In 't Veld PJ, Jeganathan S, Petrovic A, Singh P, John J, Krenn V, Weissmann F, Bange T, Musacchio A., Free PMC Article | 10/28/2017 |
| FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres. | The CENP-T/-W complex is a binding partner of the histone chaperone FACT.
Prendergast L, Müller S, Liu Y, Huang H, Dingli F, Loew D, Vassias I, Patel DJ, Sullivan KF, Almouzni G., Free PMC Article | 04/29/2017 |
| We used a synthetic system to dissect how CenH3(CENP-A) contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores | HJURP involvement in de novo CenH3(CENP-A) and CENP-C recruitment.
Tachiwana H, Müller S, Blümer J, Klare K, Musacchio A, Almouzni G. | 01/16/2016 |
| CENP-T directly interacts with Ndc80, which in turn promotes KNL1/Mis12 complex recruitment at kinetochores during chromosome segregation. | Distinct organization and regulation of the outer kinetochore KMN network downstream of CENP-C and CENP-T.
Rago F, Gascoigne KE, Cheeseman IM., Free PMC Article | 01/16/2016 |
| CSN5 regulates the stability of the inner kinetochore components CENP-T and CENP-W, providing the first direct link between CSN5 and the mitotic apparatus, highlighting the role of CSN5 as a multifunctional cell cycle regulator. | CSN5/JAB1 interacts with the centromeric components CENP-T and CENP-W and regulates their proteasome-mediated degradation.
Chun Y, Lee M, Park B, Lee S., Free PMC Article | 11/30/2013 |
| CENP-T provides a structural platform for outer kinetochore assembly. | CENP-T provides a structural platform for outer kinetochore assembly.
Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T., Free PMC Article | 04/6/2013 |
| results reveal the molecular function of CENP-T proteins and demonstrate how the Ndc80 complex is anchored to centromeres in a manner that couples chromosome movement to spindle dynamics | CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Schleiffer A, Maier M, Litos G, Lampert F, Hornung P, Mechtler K, Westermann S. | 08/18/2012 |
| The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations. | Premitotic assembly of human CENPs -T and -W switches centromeric chromatin to a mitotic state.
Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan KF., Free PMC Article | 10/29/2011 |
| CENP-T exchange into centromeres is restricted to the S-phase of the cell cycle as revealed by FRAP, suggesting a coreplicational loading mechanism. | Live-cell imaging reveals sustained centromere binding of CENP-T via CENP-A and CENP-B.
Hellwig D, Münch S, Orthaus S, Hoischen C, Hemmerich P, Diekmann S. | 01/21/2010 |
| Study demonstrates that CENP-W forms a DNA-binding complex together with the CCAN component CENP-T; this complex directly associates with nucleosomal DNA and with canonical histone H3, but not with CENP-A, in centromeric regions. | CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore.
Hori T, Amano M, Suzuki A, Backer CB, Welburn JP, Dong Y, McEwen BF, Shang WH, Suzuki E, Okawa K, Cheeseman IM, Fukagawa T. | 01/21/2010 |