| Est1 binds two adjacent, separate patches of Cdc13 with distinct pockets. The N-terminal interface of Cdc13-Est1 backstops TLC1-Ku-Sir4 to recruit telomerase. The C-terminal interface of Cdc13-Est1 is crucial for telomere maintenance in vivo. | Structural Insights into Yeast Telomerase Recruitment to Telomeres.
Chen H, Xue J, Churikov D, Hass EP, Shi S, Lemon LD, Luciano P, Bertuch AA, Zappulla DC, Géli V, Wu J, Lei M., Free PMC Article | 01/5/2019 |
| Recruitment of Est1 to telomeres via a Cdc13-Est1 fusion is sufficient to bypass the role of Ku in promoting telomere elongation. | The principal role of Ku in telomere length maintenance is promotion of Est1 association with telomeres.
Williams JM, Ouenzar F, Lemon LD, Chartrand P, Bertuch AA., Free PMC Article | 08/1/2015 |
| Autonomous localization of Est1p to the nucleus via the classical nuclear import pathway contributes to normal telomere length maintenance. | Normal telomere length maintenance in Saccharomyces cerevisiae requires nuclear import of the ever shorter telomeres 1 (Est1) protein via the importin alpha pathway.
Hawkins C, Friedman KL., Free PMC Article | 04/4/2015 |
| Esl1 has no telomere-related functions, but contributes to the regulation of adaptive gene expression responses. | Yeast hEST1A/B (SMG5/6)-like proteins contribute to environment-sensing adaptive gene expression responses.
Lai X, Beilharz T, Au WC, Hammet A, Preiss T, Basrai MA, Heierhorst J., Free PMC Article | 06/28/2014 |
| It is possible that the intramolecular G-quadruplex promoting activity of Est1 is biologically relevant in telomere replication in vivo. | Characterization of the intramolecular G-quadruplex promoting activity of Est1.
Li QJ, Tong XJ, Duan YM, Zhou JQ. | 05/4/2013 |
| There is a biochemical interaction between Est1 and the telomere binding protein Cdc13. Association of the Est1 protein with the telomerase catalytic core is not a regulatory event that contributes to telomere homeostasis. | A yeast telomerase complex containing the Est1 recruitment protein is assembled early in the cell cycle.
Tucey TM, Lundblad V., Free PMC Article | 04/27/2013 |
| the telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro | The telomeric Cdc13 protein interacts directly with the telomerase subunit Est1 to bring it to telomeric DNA ends in vitro.
Wu Y, Zakian VA., Free PMC Article | 03/10/2012 |
| The tricotetrapeptide repeat of Est1 is required for Est2-mediated telomere length maintenance. | The TPR-containing domain within Est1 homologs exhibits species-specific roles in telomerase interaction and telomere length homeostasis.
Sealey DC, Kostic AD, LeBel C, Pryde F, Harrington L., Free PMC Article | 03/3/2012 |
| These results demonstrate that one major functional role that Est1 brings to the telomerase complex is the capping or protection of telomeres. | Est1 protects telomeres and inhibits subtelomeric y'-element recombination.
Tong XJ, Li QJ, Duan YM, Liu NN, Zhang ML, Zhou JQ., Free PMC Article | 04/23/2011 |
| The authors show that the yeast telomerase subunit Est1p, known to be involved in telomerase recruitment to telomeres, can convert single-stranded telomeric G-rich DNA into a G-quadruplex structure in vitro in a Mg(2+)-dependent manner. | Yeast telomerase subunit Est1p has guanine quadruplex-promoting activity that is required for telomere elongation.
Zhang ML, Tong XJ, Fu XH, Zhou BO, Wang J, Liao XH, Li QJ, Shen N, Ding J, Zhou JQ. | 03/1/2010 |
| Maintenance of functional levels of Est2p late in the cell cycle requires that Est1p. | Two pathways recruit telomerase to Saccharomyces cerevisiae telomeres.
Chan A, Boulé JB, Zakian VA., Free PMC Article | 01/21/2010 |
| dimerization of TLC1 is a functionally conserved feature of the RNA templates utilized by reverse transcriptases of both viral and cellular origins | Functional characterization of yeast telomerase RNA dimerization.
Gipson CL, Xin ZT, Danzy SC, Parslow TG, Ly H. | 01/21/2010 |
| telomerase assembly during G1 phase is regulated by Est1p stability, but assembly is insufficient to activate telomerase at telomeres | Proteasome-dependent degradation of Est1p regulates the cell cycle-restricted assembly of telomerase in Saccharomyces cerevisiae.
Osterhage JL, Talley JM, Friedman KL. | 01/21/2010 |